Dopamine hydrochloride，Endogenous neurotransmitter (ab120565)
Key features and details
- Endogenous neurotransmitter
- CAS Number: 62-31-7
- Soluble in water to 100 mM
- Form / State: Solid
- Source: Synthetic
概述
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产品名称
Dopamine hydrochloride，Endogenous neurotransmitter -
描述
Endogenous neurotransmitter -
CAS编号
62-31-7 -
化学结构
性能
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化学名称
3,4-Dihydroxyphenethylamine hydrochloride -
分子量
189.64 -
分子式
C8H11NO2.HCl -
存放说明
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
溶解度概述
Soluble in water to 100 mM -
处理
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Toxic, refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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来源
Synthetic
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研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab120565于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| 说明 |
|---|
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Functional Studies
Use at an assay dependent concentration. |
图片
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Chemical Structure - Dopamine hydrochloride, Endogenous neurotransmitter (ab120565)2D chemical structure image of ab120565, Dopamine hydrochloride, Endogenous neurotransmitter
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Functional Studies - Dopamine hydrochloride, Endogenous neurotransmitter (ab120565)ab32088 staining MEK1 (phospho S218 + S222) in SKNSH cells treated with dopamine hydrochloride (ab120565), by ICC/IF. Increase in MEK1 (phospho S218 + S222) expression correlates with increased concentration of dopamine hydrochloride, as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120565 (dopamine hydrochloride) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab32088 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
文献 (1)
ab120565 被引用在 1 文献中.
- Raffaelli FM et al. Dopamine receptor D1- and D2-agonists do not spark brown adipose tissue thermogenesis in mice. Sci Rep 10:20203 (2020). PubMed: 33214601