Diclofenac sodium salt，cyclo-oxygenase抑制剂(ab120621)
Key features and details
- Potent cyclo-oxygenase inhibitor
- CAS Number: 15307-79-6
- Soluble in water to 50 mM (at room temperature) and in water to 100 mM (with warming)
- Form / State: Solid
- Source: Synthetic
概述
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产品名称
Diclofenac sodium salt，cyclo-oxygenase抑制剂 -
描述
Potent cyclo-oxygenase抑制剂 -
生物学描述
Potent non-selective COX inhibitor (IC50 values are 75 and 29 nM at COX-1 and COX-2, respectively). Potent inhibitor of phospholipase A2 in vitro and in vivo. Activates the nitric oxide-cyclic GMP-potassium channel pathway. Shows anti-inflammatory, antipyretic and analgesic effects.
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CAS编号
15307-79-6 -
化学结构
性能
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化学名称
2-[(2,6-Dichlorophenyl)amino]benzeneacetic acid sodium salt -
分子量
318.13 -
分子式
C14H10Cl2NNaO2 -
存放说明
Store at Room Temperature. Store under desiccating conditions. The product can be stored for up to 12 months. -
溶解度概述
Soluble in water to 50 mM (at room temperature) and in water to 100 mM (with warming) -
处理
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Toxic, refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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来源
Synthetic
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研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab120621于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| 说明 |
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Functional Studies
Use at an assay dependent concentration. |
图片
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Chemical Structure - Diclofenac sodium salt, cyclo-oxygenase inhibitor (ab120621)2D chemical structure image of ab120621, Diclofenac sodium salt, cyclo-oxygenase inhibitor
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Immunocytochemistry/ Immunofluorescence - Diclofenac sodium salt, cyclo-oxygenase inhibitor (ab120621)ab96732 staining Prostaglandin dehydrogenase 1 in MCF7 cells treated with diclofenac sodium salt (ab120621), by ICC/IF. Increase in Prostaglandin dehydrogenase 1 expression correlates with increased concentration of diclofenac sodium salt as described in literature.
The cells were incubated at 37°C for 6h in media containing different concentrations of ab120621 (diclofenac sodium salt) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab96732 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
文献 (1)
ab120621 被引用在 1 文献中.
- Pritchard R et al. Celecoxib inhibits mitochondrial O2 consumption, promoting ROS dependent death of murine and human metastatic cancer cells via the apoptotic signalling pathway. Biochem Pharmacol 154:318-334 (2018). PubMed: 29800556