DAPT，gamma-Secretase抑制剂(ab120633)
Key features and details
- γ-Secretase inhibitor
- CAS Number: 208255-80-5
- Purity: > 99%
- Soluble in DMSO to 100 mM
- Form / State: Solid
- Source: Synthetic
概述
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产品名称
DAPT，gamma-Secretase抑制剂 -
描述
γ-Secretase抑制剂 -
生物学描述
γ-Secretase inhibitor. Inhibits Notch signalling in vitro. Inhibits total Aβ and Aβ42 production in human primary cultures (IC50 values are 115 and 200 nM, respectively) with no effect on APPα and APPβ levels. Blood-brain barrier permeable. 3D Growth matrix component and component of cerebral organoid differentiation media.
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纯度
> 99% -
CAS编号
208255-80-5 -
化学结构
性能
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化学名称
N-[N-(3,5-Difluorophenacetyl-L-alanyl)]-(S)-phenylglycine t-butyl ester -
分子量
432.47 -
分子式
C23H26F2N2O4 -
PubChem识别号
5311272 -
存放说明
Store at +4°C. The product can be stored for up to 12 months. -
溶解度概述
Soluble in DMSO to 100 mM -
处理
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
C[C@@H](C(=O)N[C@@H](C1=CC=CC=C1)C(=O)OC(C)(C)C)NC(=O)CC2=CC(=CC(=C2)F)F -
来源
Synthetic
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研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab120633于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| 说明 |
|---|
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Functional Studies
Use at an assay dependent concentration. |
图片
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Chemical Structure - DAPT, gamma-Secretase inhibitor (ab120633)2D chemical structure image of ab120633, DAPT, gamma-Secretase inhibitor
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Functional Studies - DAPT, gamma-Secretase inhibitor (ab120633)ab81283 staining AKT1 (phospho S473) in MCF7 cells treated with DAPT (ab120633), by ICC/IF. Decrease in expression of AKT1 (phospho S473) correlates with increased concentration of DAPT, as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120633 (DAPT) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab81283 (1/200 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue. -
Functional Studies - DAPT, gamma-Secretase inhibitor (ab120633)This image is courtesy of an Abreview submitted by a verified customerKc167 cells were treated with varying concentrations of DAPT (ab120633) in DMSO for 16hr at 25°C; DMSO only was used as the negative control. The cells were further incubated for 30 minutes with 4mM EGTA in PBS (in the presence of DAPT), and were then lysed for analysis. To measure Notch activity, Notch targets E(spl)mβ-HLH and E(spl)m3-HLH mRNA levels were assayed. Data shows the fold change of mRNA levels of E(spl)mβ-HLH and E(spl)m3-HLH under different conditions, normalised to DMSO treatment (negative control). Notch activation by EGTA is abrogated by treatment with DAPT.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
文献 (25)
ab120633 被引用在 25 文献中.
- Coppo R et al. Distinct but interchangeable subpopulations of colorectal cancer cells with different growth fates and drug sensitivity. iScience 26:105962 (2023). PubMed: 36718360
- Kim HJ et al. Generation of multilineage liver organoids with luminal vasculature and bile ducts from human pluripotent stem cells via modulation of Notch signaling. Stem Cell Res Ther 14:19 (2023). PubMed: 36737811
- Wang Y et al. Notch4 participates in mesenchymal stem cell-induced differentiation in 3D-printed matrix and is implicated in eccrine sweat gland morphogenesis. Burns Trauma 11:tkad032 (2023). PubMed: 37397510
- Najle SR et al. Stepwise emergence of the neuronal gene expression program in early animal evolution. Cell 186:4676-4693.e29 (2023). PubMed: 37729907
- Ngan SY et al. Stage-Specific Generation of Human Pluripotent Stem Cell Derived Lung Models to Measure CFTR Function. Curr Protoc 2:e341 (2022). PubMed: 35025140