D4476，CK1抑制剂(ab120220)
Key features and details
- CK1 inhibitor
- CAS Number: 301836-43-1
- Soluble in DMSO to 100 mM
- Form / State: Solid
- Source: Synthetic
概述
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产品名称
D4476，CK1抑制剂 -
描述
CK1抑制剂 -
生物学描述
Selective cell-permeable inhibitor of casein kinase 1 (CK1) . (IC50 values are 0.3 (CK1), 0.5 (ALK5), 9.1 (PKd1) and 5.8 μM (p38αMAPK)). Displays selectivity over many other protein kinases.
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CAS编号
301836-43-1 -
化学结构
性能
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化学名称
4-[4-(2,3-Dihydro-1,4-benzodioxin-6-yl)-5-(2-pyridinyl)-1H-imidazol-2-yl]benzamide -
分子量
398.41 -
分子式
C23H18N4O3 -
PubChem识别号
6419753 -
存放说明
Store at +4°C. The product can be stored for up to 12 months. -
溶解度概述
Soluble in DMSO to 100 mM -
处理
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
NC(=O)c1ccc(cc1)c3nc(c2ccccn2)c(n3)c4ccc5OCCOc5c4 -
来源
Synthetic
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研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab120220于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| 说明 |
|---|
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Functional Studies
Use at an assay dependent concentration. |
图片
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Chemical Structure - D4476, CK1 inhibitor (ab120220)2D chemical structure image of ab120220, D4476, CK1 inhibitor
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Functional Studies - D4476, CK1 inhibitor (ab120220)ab38511 staining βcatenin (phospho Thr42 + Ser45) in SK-N-SH cells treated with D4476 (ab120220), by ICC/IF. Decrease in expression of βcatenin (phospho Thr42 + Ser45) correlates with increased concentration of D4476, as described in literature.
The cells were incubated at 37°C for 2h in media containing different concentrations of ab120220 (D4476) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab38511 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
文献 (6)
ab120220 被引用在 6 文献中.
- Behrouj H & Mokarram P BAMLET (Bovine α-lactalbumin made lethal to tumor cells) inhibits autophagy flux and induces apoptosis via down-regulation of protein kinase CK1α and attenuation of the AKT/p-ß-catenin (S552) pathway in RAS-mutated human colorectal HCT 116 cells. Iran J Basic Med Sci 26:1212-1219 (2023). PubMed: 37736507
- Feng D et al. Analysis of the Ubiquitination and Phosphorylation of Vangl Proteins. Bio Protoc 12:N/A (2022). PubMed: 36353719
- Feng D et al. Regulation of Wnt/PCP signaling through p97/VCP-KBTBD7-mediated Vangl ubiquitination and endoplasmic reticulum-associated degradation. Sci Adv 7:N/A (2021). PubMed: 33990333
- Siri M et al. Casein Kinase-1-Alpha Inhibitor (D4476) Sensitizes Microsatellite Instable Colorectal Cancer Cells to 5-Fluorouracil via Authophagy Flux Inhibition. Arch Immunol Ther Exp (Warsz) 69:26 (2021). PubMed: 34536148
- Kuga T et al. Casein kinase 1 is recruited to nuclear speckles by FAM83H and SON. Sci Rep 6:34472 (2016). PubMed: 27681590
- Chia R et al. Phosphorylation of LRRK2 by casein kinase 1a regulates trans-Golgi clustering via differential interaction with ARHGEF7. Nat Commun 5:5827 (2014). PubMed: 25500533