CNQX，AMPA / kainate拮抗剂(ab120017)
Key features and details
- AMPA / kainate antagonist
- CAS Number: 115066-14-3
- Soluble in DMSO to 100 mM
- Form / State: Solid
- Source: Synthetic
概述
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产品名称
CNQX，AMPA / kainate拮抗剂 -
描述
AMPA / kainate拮抗剂 -
生物学描述
Potent, competitive AMPA / kainate receptor antagonist. Also antagonist at NMDA receptor glycine site.
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CAS编号
115066-14-3 -
化学结构
性能
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化学名称
6-Cyano-7-nitroquinoxaline-2,3-dione -
分子量
232.16 -
分子式
C9H4N4O4 -
PubChem识别号
3721046 -
存放说明
Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
溶解度概述
Soluble in DMSO to 100 mM -
处理
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
[O-][N+](=O)c1cc2NC(=O)C(=O)Nc2cc1C#N -
来源
Synthetic
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研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab120017于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
Functional Studies
Use at an assay dependent concentration. |
图片
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Chemical Structure - CNQX, AMPA / kainate antagonist (ab120017)2D chemical structure image of ab120017, CNQX, AMPA / kainate antagonist
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Immunocytochemistry/ Immunofluorescence - CNQX, AMPA / kainate antagonist (ab120017)ab96379 staining MEK1 (phospho S298) in SK-N-SH cells treated with CNQX (ab120017), by ICC/IF. Decrease in MEK1 (phospho S298) expression correlates with increased concentration of CNQX, as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120017 (CNQX) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab96379 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. -
Cellular activation - CNQX, AMPA / kainate antagonist (ab120017)Image from Ildiko P, et al. Plos One, 8(3), e57694. Fig 4a,; doi: 110.1371/journal.pone.0057694Left and Middle: Representative IOS amplitude map and field response curve under control condition and under application of 20 µM CNQX, respectively. The colorbar indicates the maximum change of the transmittance compared to the resting light intensity. A Right: Spatial visualization of the percentage of control changes of IOS signal caused by CNQX application.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
文献 (72)
ab120017 被引用在 72 文献中.
- Fan S et al. Isoliquiritigenin modulates the activity of LTS and non-LTS cells in the ventrolateral preoptic area via GABAA receptors. Heliyon 9:e20620 (2023). PubMed: 37876454
- Mouradian GC et al. Patch-to-Seq and Transcriptomic Analyses Yield Molecular Markers of Functionally Distinct Brainstem Serotonin Neurons. Front Synaptic Neurosci 14:910820 (2022). PubMed: 35844900
- Kleidonas D & Vlachos A Scavenging Tumor Necrosis Factor a Does Not Affect Inhibition of Dentate Granule Cells Following In Vitro Entorhinal Cortex Lesion. Cells 10:N/A (2021). PubMed: 34831454
- Sanders SS et al. The palmitoyl acyltransferase ZDHHC14 controls Kv1-family potassium channel clustering at the axon initial segment. Elife 9:N/A (2020). PubMed: 33185190
- Mangieri LR et al. A neural basis for antagonistic control of feeding and compulsive behaviors. Nat Commun 9:52 (2018). PubMed: 29302029