CGP 57380，MNK抑制剂(ab120365)
Key features and details
- MNK inhibitor
- CAS Number: 522629-08-9
- Soluble in DMSO to 100 mM
- Form / State: Solid
- Source: Synthetic
概述
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产品名称
CGP 57380，MNK抑制剂 -
描述
MNK抑制剂 -
生物学描述
Low-molecular-weight kinase inhibitor of MAP-kinase interacting kinase-1 (MNK1) and MNK2 (IC50 values are 0.87 and 1.6 μM, respectively). Inhibits a range of other kinases (IC50 values in μM shown in brackets) including: CKI (0.51) Aurora B (2.5) DYRK3 (3.2) SGK1( 2.7) BRSK2 (1.1) and Lck (2.5).
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CAS编号
522629-08-9 -
化学结构
性能
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化学名称
N3-(4-Fluorophenyl)-1H-pyrazolo-[3,4-d]pyrimidine-3,4-diamine -
分子量
244.23 -
分子式
C11H9FN6 -
PubChem识别号
11644425 -
存放说明
Store at +4°C. The product can be stored for up to 12 months. -
溶解度概述
Soluble in DMSO to 100 mM -
处理
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Refer to SDS for further information
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
Fc1ccc(cc1)Nc3nnc2ncnc(N)c23 -
来源
Synthetic
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研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab120365于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| 说明 |
|---|
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Functional Studies
Use at an assay dependent concentration. |
图片
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Chemical Structure - CGP 57380, MNK inhibitor (ab120365)2D chemical structure image of ab120365, CGP 57380, MNK inhibitor
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Immunocytochemistry/ Immunofluorescence - CGP 57380, MNK inhibitor (ab120365)ab76256 staining eIF4E (phospho S209) in serum starved HEK193 cells treated with CGP 57380 (ab120365), by ICC/IF. Decrease in eIF4E (phospho S209) expression correlates with increased concentration of CGP 57380, as described in literature.
The cells were incubated at 37°C for 1h in media containing different concentrations of ab120365 (CGP 57380) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab76256 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
文献 (2)
ab120365 被引用在 2 文献中.
- Moore CE et al. MNK1 and MNK2 mediate adverse effects of high-fat feeding in distinct ways. Sci Rep 6:23476 (2016). PubMed: 27087055
- Leong SY et al. The role of Misshapen NCK-related kinase (MINK), a novel Ste20 family kinase, in the IRES-mediated protein translation of human enterovirus 71. PLoS Pathog 11:e1004686 (2015). PubMed: 25747578