Calmidazolium chloride，Calmodulin抑制剂(ab120658)
Key features and details
- Calmodulin inhibitor
- CAS Number: 57265-65-3
- Purity: > 98%
- Soluble in ethanol to 100 mM and in DMSO to 100 mM
- Form / State: Solid
- Source: Synthetic
概述
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产品名称
Calmidazolium chloride，Calmodulin抑制剂 -
描述
Calmodulin抑制剂 -
别名
- R 24571
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生物学描述
Calmodulin inhibitor (IC50 = 10 nM). Elevates intracellular calcium, independent of calmodulin inhibition. Additionally inhibits adenylyl cylase activity.
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纯度
> 98% -
CAS编号
57265-65-3 -
化学结构
性能
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化学名称
3-[Bis(4-chlorophenyl)methyl]-1-[2-(2,4-dichlorophenyl)-2-[(2,4-dichlorophenyl)methoxy)ethyl]-1H-imidazolium chloride -
分子量
687.70 -
分子式
C31H23Cl7N2O -
PubChem识别号
644274 -
存放说明
Store at -20°C. The product can be stored for up to 12 months. -
溶解度概述
Soluble in ethanol to 100 mM and in DMSO to 100 mM -
处理
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Refer to SDS for further information
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
C1=CC(=CC=C1C(C2=CC=C(C=C2)Cl)N3C=C[N+](=C3)CC(C4=C(C=C(C=C4)Cl)Cl)OCC5=C(C=C(C=C5)Cl)Cl)Cl.[Cl-] -
来源
Synthetic
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研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab120658于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
Functional Studies
Use at an assay dependent concentration. |
图片
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Chemical Structure - Calmidazolium chloride, Calmodulin inhibitor (ab120658)2D chemical structure image of ab120658, Calmidazolium chloride, Calmodulin inhibitor
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Immunocytochemistry/ Immunofluorescence - Calmidazolium chloride, Calmodulin inhibitor (ab120658)ab48394 staining LC3B (LC3-II) in HeLa cells treated with calmidazolium chloride (ab120658), by ICC/IF. Increase of LC3B (LC3-II) expression correlates with increased concentration of calmidazolium chloride, as described in literature.
The cells were incubated at 37°C for 6h in media containing different concentrations of ab120658 (calmidazolium chloride) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab48394 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
文献 (0)
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