7-Chlorokynurenic acid sodium salt，NMDA receptor glycine site拮抗剂(ab120255)
Key features and details
- NMDA receptor glycine site antagonist; water soluble
- CAS Number: 1263094-00-3
- Purity: > 99%
Soluble in water to 100 mM
- Form / State: Solid
- Source: Synthetic
概述
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产品名称
7-Chlorokynurenic acid sodium salt，NMDA receptor glycine site拮抗剂 -
描述
NMDA receptor glycine site拮抗剂; water soluble -
生物学描述
Potent NMDA receptor glycine site antagonist. Water soluble form.
Also available in simple stock solutions (ab146692) - add 1 ml of water to get an exact, ready-to-use concentration. -
纯度
> 99% -
CAS编号
1263094-00-3 -
化学结构
性能
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化学名称
7-Chloro-4-hydroxyquinoline-2-carboxylic acid sodium salt
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分子量
245.60 -
PubChem识别号
52974249 -
存放说明
Store at Room Temperature. Store under desiccating conditions. The product can be stored for up to 12 months. -
溶解度概述
Soluble in water to 100 mM
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处理
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
O=C(O[Na])c1cc(O)c2ccc(Cl)cc2n1 -
来源
Synthetic
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研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab120255于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
Functional Studies
Use at an assay dependent concentration. |
图片
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Chemical Structure - 7-Chlorokynurenic acid sodium salt, NMDA receptor glycine site antagonist (ab120255)2D chemical structure image of ab120255, 7-Chlorokynurenic acid sodium salt, NMDA receptor glycine site antagonist
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Functional Studies - 7-Chlorokynurenic acid sodium salt, NMDA receptor glycine site antagonist (ab120255)ab12416 staining cGMP in SKNSH cells treated with 7-Chlorokynurenic acid sodium salt (ab120255), by ICC/IF. Decrease in cGMP expression correlates with increased concentration of 7-Chlorokynurenic acid sodium salt, as described in literature.
The cells were incubated at 37°C for 30 minutes in media containing different concentrations of ab120255 (7-Chlorokynurenic acid sodium salt) in DMSO. Some samples where then further incubated with 1 mM glutamate (ab120049) for 15 minutes and all samples were fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab12416 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
文献 (3)
ab120255 被引用在 3 文献中.
- Biane C et al. Developmental emergence of two-stage nonlinear synaptic integration in cerebellar interneurons. Elife 10:N/A (2021). PubMed: 34730085
- Raghunatha P et al. Microglial NMDA receptors drive pro-inflammatory responses via PARP-1/TRMP2 signaling. Glia 68:1421-1434 (2020). PubMed: 32036619
- Hansen KB et al. Implementation of a fluorescence-based screening assay identifies histamine H3 receptor antagonists clobenpropit and iodophenpropit as subunit-selective N-methyl-D-aspartate receptor antagonists. J Pharmacol Exp Ther 333:650-62 (2010). PubMed: 20197375
