Zinc Quantification试剂盒(Fluorometric) (ab176725)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Cell culture extracts, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 13 ng/ml
概述
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产品名称
Zinc Quantification试剂盒(Fluorometric)
参阅全部 Zinc 试剂盒 -
检测方法
Fluorescent -
样品类型
Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Tissue Extracts -
检测类型
Quantitative -
灵敏度
>= 13 ng/ml -
种属反应性
与反应: Mammals, Other species -
产品概述
Abcam's Zinc Quantification Kit (Fluorometric) (ab176725) provides a robust method for detecting zinc concentration in biological samples using our proprietary Zn Detector, in which Zinc binds to the probe with enhanced fluorescence at Ex/Em= 485/525 nm. The Zinc probe exhibits a large increase in fluorescence in response to Zn2+ (greater than 200~300 folds). It has high Zn2+ -specificity with little responses to other metals, including Ca2+, Mg2+, Mn2+, and Cu2+. The assay can be used with biological samples such as serum, plasma, and urine with detection sensitivity at 0.2 µM (13 ng/mL).
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说明
Zinc is an essential trace mineral element that plays an important role in a number of biological processes. It is an essential factor required for many enzymes, protein structures, and control of genetic expression. Zinc status also affects basic processes of cell division, growth, differentiation, development, and aging. Clinical signs of zinc deficiency include acrodermatitis, low immunity, diarrhea, poor healing, stunting, hypogonadism, fetal growth failure, teratology and abortion. Simple, direct and automation-ready procedures for measuring zinc ion are highly desirable in research and drug discovery.
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平台
Microplate reader
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 200 tests Assay Buffer 1 x 15ml Zn Detector 1 vial ZnCl2 Standard 1 vial -
研究领域
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相关性
Zinc, a metallic chemical element, symbol Zn and atomic number 30 is chemically similar to Magnesium due to its similar size and sole oxidation state of +2. Zinc is an essential mineral of great biological significance since many enzymes require it as an essential cofactor. Examples of zinc’s biological roles include signal transduction, gene expression, regulation of apoptosis, synaptic plasticity and prostate gland function. -
别名
- Zn
- Zn++
- Zn2+
图片
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ZnCl2 dose response curve
ZnCl2 dose response was measured on a 96-well black plate with Zinc Quantification Kit (Fluorometric) (ab176725). As low as ~ 0.2 µM Zn2+ can be detected with 5 minutes incubation time (n=3).
数据表及文件
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SDS download
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Datasheet download
文献 (11)
ab176725 被引用在 11 文献中.
- Lapizco-Encinas BH & Zhang YV Microfluidic systems in clinical diagnosis. Electrophoresis 44:217-245 (2023). PubMed: 35977346
- Iring-Varga B et al. The antidepressant effect of short- and long-term zinc exposition is partly mediated by P2X7 receptors in male mice. Front Pharmacol 14:1241406 (2023). PubMed: 37908978
- Aigner GP et al. Wound healing and Cadmium detoxification in the earthworm Lumbricus terrestris - a potential case for coelomocytes? Front Immunol 14:1272191 (2023). PubMed: 38116011
- Olivares-González L et al. NUTRARET: Effect of 2-Year Nutraceutical Supplementation on Redox Status and Visual Function of Patients With Retinitis Pigmentosa: A Randomized, Double-Blind, Placebo-Controlled Trial. Front Nutr 9:847910 (2022). PubMed: 35387197
- Braakhuis A et al. A Modern Flexitarian Dietary Intervention Incorporating Web-Based Nutrition Education in Healthy Young Adults: Protocol for a Randomized Controlled Trial. JMIR Res Protoc 10:e30909 (2021). PubMed: 34931994