Urokinase type Plasminogen活化剂人Chromogenic Activity Assay试剂盒(ab108915)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Cell culture supernatant, Cell Lysate, Plasma, Serum, Tissue
- Sensitivity: 0.0051 U/ml
概述
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产品名称
Urokinase type Plasminogen活化剂人Chromogenic Activity Assay试剂盒
参阅全部 Urokinase 试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Serum, Plasma, Tissue, Cell Lysate -
检测类型
Enzyme activity (quantitative) -
灵敏度
0.0051 U/ml -
种属反应性
与反应: Human -
产品概述
Urokinase type plasminogen activator (uPA) is a highly restricted serine protease that converts the zymogen plasminogen to active plasmin, a broad-spectrum serine proteinase capable of degrading most of the major protein components of the extracellular matrix. Binding of uPA to its receptor and subsequent uPA mediated pericellular proteolysis are involved in many process including cell migration and tissue remodelling in angiogenesis, atherogenesis, tumor cell metastasis, and ovulation. High level of uPA is a poor prognostic marker for aggressive breast cancer, aggressive prostate cancer, bladder cancer and gastric cancer.
ab108915 uPA Human Chromogenic Activity Assay Kit is developed to determine Human uPA activity in plasma and cell culture supernatants. The assay measures the ability of uPA to activate the plasminogen to plasmin in coupled or indirect assays that contain uPA, plasminogen, and a plasmin-specific synthetic substrate. The amount of plasmin produced is quantitated using a highly specific plasmin substrate releasing a yellow para-nitroaniline (pNA) chromophore. The change in absorbance of the pNA in the reaction solution at 405 nm is directly proportional to the uPA enzymatic activity.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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平台
Microplate reader
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 100 tests Assay Diluent 1 x 30ml Human Plasminogen 1 x 2 vials Human uPA Standard 1 vial Microplate 1 unit Plasmin Substrate 1 x 2 vials Sealing Tapes 3 units -
研究领域
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功能
Specifically cleave the zymogen plasminogen to form the active enzyme plasmin. -
组织特异性
Expressed in the prostate gland and prostate cancers. -
序列相似性
Belongs to the peptidase S1 family.
Contains 1 EGF-like domain.
Contains 1 kringle domain.
Contains 1 peptidase S1 domain. -
翻译后修饰
Phosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding. -
细胞定位
Secreted. - Information by UniProt
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别名
- ATF
- ATF uPA
- BDPLT5
see all -
数据库链接
- Entrez Gene: 5328 Human
- Omim: 191840 Human
- SwissProt: P00749 Human
- Unigene: 77274 Human
图片
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Indirect ELISA - Urokinase type plasminogen activator Human Chromogenic Activity Assay Kit (ab108915)
uPA measured in biologicals showing quantity (IU) per mL of sample tested.
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Indirect ELISA - Urokinase type plasminogen activator Human chromogenic activity assay kit example standard curve (ab108915)uPA Chromogenic Activity Standard Curve (For High uPA Activity Samples)
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Indirect ELISA - Urokinase type plasminogen activator Human chromogenic activity assay kit example standard curve (ab108915)uPA Chromogenic Activity Standard Curve (For Low uPA Activity Samples)
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab108915 被引用在 2 文献中.
- Hohensinner PJ et al. PAI-1 (Plasminogen Activator Inhibitor-1) Expression Renders Alternatively Activated Human Macrophages Proteolytically Quiescent. Arterioscler Thromb Vasc Biol N/A:N/A (2017). PubMed: 28818858
- Gupta V et al. Glaucoma is associated with plasmin proteolytic activation mediated through oxidative inactivation of neuroserpin. Sci Rep 7:8412 (2017). PubMed: 28827627