Triglyceride Assay试剂盒(Fluorometric，Reducing Samples) (ab178780)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Adherent cells, Hep Plasma, Saliva, Serum, Suspension cells, Tissue
- Sensitivity: 0.4 µM
概述
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产品名称
Triglyceride Assay试剂盒(Fluorometric，Reducing Samples)
参阅全部 Triglyceride 试剂盒 -
检测方法
Fluorescent -
样品类型
Saliva, Serum, Tissue, Adherent cells, Suspension cells, Hep Plasma -
检测类型
Quantitative -
灵敏度
< 0.4 µM -
检测时间
1h 00m -
种属反应性
与反应: Mammals, Other species -
产品概述
Triglyceride Assay Kit (ab178780) is a sensitive, easy assay to measure triglyceride concentration in mammalian samples. In this triglyceride assay protocol, triglycerides are hydrolyzed to glycerol and fatty acid. The glycerol reacts with Triglyceride Enzyme Mix to form an intermediate product which in turn reacts with the probe and developer to generate fluorescence (Ed/Em 535/587 nm). The generated fluorescence is directly proportional to the amount of triglycerides.
Triglyceride Assay Kit (Fluorometric, Reducing Samples) ab178780 is suitable for measuring triglyceride levels in samples which contain reducing substances that may interfere with oxidase-based assays such as Triglyceride Assay Kit (ab65336).
Triglyceride assay protocol summary:
- add samples and standards to wells
- add lipase and incubate for 20 min
- add reaction mix and incubate for 30 min
- analyze with a microplate reader.
Please note: the general range is 0-10 nmol (colorimetric) and 0-1 nmol (fluorometric).
Target background
Triglycerides are the main constituent of vegetable oil, animal fat, LDL and VLDL, and play an important role as transporters of fatty acids as well as serving as an energy source. Triglycerides are broken down into fatty acids and glycerol, after which both can serve as substrates for energy producing and metabolic pathways. High blood levels of triglycerides are implicated in atherosclerosis, heart disease and stroke as well as in pancreatitis.
Review our Metabolism Assay Guide to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also about how to assay metabolic function in live cells using your plate reader.
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说明
This product is manufactured by BioVision, an Abcam company and was previously called K614 PicoProbe™ Triglyceride (TG) Fluorometric Assay Kit. K614-100 is the same size as the 100 test size of ab178780.
How other researchers have used Triglyceride Assay Kit ab178780
The Triglyceride assay kit has been used in publications in a variety of sample types, including:
- Human: Hepatocyte cell/cell line extract1,2
- Mouse: Plasma3, Skeletal muscle extract4, Serum5
References: 1 - Liu Q et al. 2021, 2 - Hu LK et al. 2021, 3 - Panajatovic MV et al, 4 - Panajatovic MV et al, 5 - Maier JI et al. 2021
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the SDS download section.
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平台
Microplate reader
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 100 tests Assay Buffer V 1 x 25ml Cholesterol Esterase 1 vial PicoProbe I 1 x 0.4ml Substrate Mix I 1 vial Triglyceride Enzyme Mix 1 vial Triglyceride Standard 1 x 0.3ml -
研究领域
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别名
- TG
- Triacylglyceride
- triglycerides
图片
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Triglyceride Standard Curve
Example of triglyceride standard curve following assay protocol. Please note that this is an example curve and should not be used for experimental purposes.
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Measurement of triglyceride levels in samplesMesurament of TG levels in rat liver (~15 µg), human serum (1 µL) and saliva (10 µL). Assays were performed according to kit protocol.
数据表及文件
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SDS download
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Datasheet download
文献 (16)
ab178780 被引用在 16 文献中.
- Bordat C et al. Validation of Knock-Out Caco-2 TC7 Cells as Models of Enterocytes of Patients with Familial Genetic Hypobetalipoproteinemias. Nutrients 15:N/A (2023). PubMed: 36771214
- Easton ZJW et al. The impact of hyperglycemia upon BeWo trophoblast cell metabolic function: A multi-OMICS and functional metabolic analysis. PLoS One 18:e0283118 (2023). PubMed: 36930661
- Patitucci C et al. Mtfp1 ablation enhances mitochondrial respiration and protects against hepatic steatosis. Nat Commun 14:8474 (2023). PubMed: 38123539
- Getiye Y et al. Dysregulated lipolysis and lipophagy in lipid droplets of macrophages from high fat diet-fed obese mice. J Cell Mol Med 26:4825-4836 (2022). PubMed: 35962606
- Guibourdenche M et al. Perinatal exposure to chlorpyrifos and/or a high-fat diet is associated with liver damage in male rat offspring. Cells Dev 166:203678 (2021). PubMed: 33994353