Total Collagen Assay试剂盒(Perchlorate-Free) (ab222942)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 3 hr
- Sample type: Serum, Tissue Homogenate, Urine
概述
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产品名称
Total Collagen Assay试剂盒(Perchlorate-Free)
参阅全部 Collagen 试剂盒 -
检测方法
Colorimetric -
样品类型
Urine, Serum, Tissue Homogenate -
检测类型
Quantitative -
范围
0.5 µg - 10 µg -
检测时间
3h 00m -
种属反应性
与反应: Mammals, Other species -
产品概述
Total Collagen Assay Kit (Perchlorate-Free) (ab222942) provides a quick and convenient method to detect collagen in tissue lysates as well as biological fluids such as urine or serum.
The collagen assay protocol is based on the Alkaline hydrolysis of samples to yield free Hydroxyproline. The released hydroxyproline is oxidized to form a reaction intermediate, which further in reaction forms brightly-colored chromophore that can be easily detected at OD 560 nm.
The assay employs a proprietary developer solution to accurately measure collagen in hydrolysates without the use of hazardous perchloric acid, removing the need for any special handling and waste-disposal protocols. The assay can detect as low as of 0.5 µg collagen/well.
Collagen assay protocol summary:
- hydrolyze samples and standard with concentrated NaOH for 1 hr at 120°C
- cool on ice and neutralize with concentrated HCL
- centrifuge at 10,000g for 5 min to pellet debris
- add samples and standards to wells
- evaporate wells to dryness at 65°C
- add oxidation mix and incubate for 20 min
- add developer and incubate for 5 min
- add DMAB concentrate and incubate for 45 min at 65°C
- analyze absorbance at 560 nm with a microplate reader -
说明
This product is manufactured by BioVision, an Abcam company and was previously called K406 Total Collagen Assay Kit (Perchlorate-Free). K406-100 is the same size as the 100 test size of ab222942.
Collagen is the most abundant insoluble protein found in the extracellular matrix and connective tissues. It can be found in skin, tendons, bone, cartilage, muscle, vitreous humor and ligaments, among other tissues. There are more than sixteen - well characterized types of collagens, but types I, II and III collagen comprise more than 80% content in mammals.
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平台
Microplate reader
性能
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存放说明
Please refer to protocols. -
组件 100 tests Chloramine T Concentrate 1 x 600µl Collagen I Standard (3 mg/ml) 1 x 200µl Developer Solution I 1 x 5ml DMAB Concentrate 1 x 5ml Microplate Sealing Film 1 unit Oxidation Buffer 1 x 10ml
图片
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Total Collagen Assay Kit (Perchlorate-Free) (ab222942)
Estimation of total collagen content in rat tissues. Rat leg muscle, spleen, heart and lung samples were prepared followind the sample preparation procedure described in the protocol (homegenized in ddH2O, hydrolyzed with 10 N NaOH for 1 hour at 120°C and neutralized with 10 N HCl). For each sample, 10 μL of the final neutralized hydrolysate was assayed. Total Collagen levels (calculated as μg collagen/mg wet tissue) for the samples were: 13.80 +- 0.73 ug/mg for muscle, 3.77 +- 0.54 ug/mg for spleen, 4.55 +- 0.81 ug/mg for heart and 12.51 +- 1.27 ug/mg for lung. Data are mean ± SEM of 3-4 replicates.
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Total Collagen Assay Kit (Perchlorate-Free) (ab222942)Typical hydrolyzed Collagen I standard calibration curve.
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Total Collagen Assay Kit (Perchlorate-Free) (ab222942)Correlation between collagen standard curve absorbance values obtained using a classical perchlorate-based assay kit and perchlorate-free Total Collagen Assay Kit (Colorimetric) (ab222942).
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab222942 被引用在 6 文献中.
- Lee JK et al. Effect of Muscle Cell Preservation on Viability and Differentiation of Hamstring Tendon Graft In Vitro. Cells 10:N/A (2021). PubMed: 33801626
- Tian Z et al. Promoting roles of KLF5 in myocardial infarction in mice involving microRNA-27a suppression and the following GFPT2/TGF-ß/Smad2/3 axis activation. Cell Cycle 20:874-893 (2021). PubMed: 33910455
- Klak M et al. Impact of Porcine Pancreas Decellularization Conditions on the Quality of Obtained dECM. Int J Mol Sci 22:N/A (2021). PubMed: 34209772
- Meador WD et al. The tricuspid valve also maladapts as shown in sheep with biventricular heart failure. Elife 9:N/A (2020). PubMed: 33320094
- Chang L et al. Magnoflorine Ameliorates Inflammation and Fibrosis in Rats With Diabetic Nephropathy by Mediating the Stability of Lysine-Specific Demethylase 3A. Front Physiol 11:580406 (2020). PubMed: 33414721
- Shati AA & El-Kott AF Acylated ghrelin prevents doxorubicin-induced cardiac intrinsic cell death and fibrosis in rats by restoring IL-6/JAK2/STAT3 signaling pathway and inhibition of STAT1. Naunyn Schmiedebergs Arch Pharmacol 392:1151-1168 (2019). PubMed: 31093684