TMRE-Mitochondrial膜Potential Assay试剂盒

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Functional Studies - TMRE-Mitochondrial Membrane Potential Assay Kit (AB113852)

Analysis of TMRE staining using a fluorescent plate reader and a microplate.

Chart showing mean fluorescent intensity +/- standard deviation from quadruplicate measurements of 400 nM TMRE stained Jurkat cells in a 96-well microplate +/- treatment with FCCP.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - TMRE-Mitochondrial Membrane Potential Assay Kit (AB113852)

A : HeLa cells (adherent) were cultured on coverslips and stained with ab113852 (200nM TMRE) for 20 minutes in media, washed briefly with PBS and immediately imaged. B : Jurkat cells (suspension) were stained and washed as above and then transferred to a slide and immobilized under a coverslip for imaging.

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PubMed

Functional Studies - TMRE-Mitochondrial Membrane Potential Assay Kit (AB113852)

Mitochondrial membrane potential was measured using ab113852.

P19 neurons (750 cells/mm²) were exposed to MDMA on days 7–9 in serum-free medium for 10 min up to 48 hours. The positive control FCCP (carbonyl cyanide-p-trifluoromethoxyphenylhydrazone), an uncoupler of mitochondrial oxidative phosphorylation, was applied at the concentration of 5 μM for 10 min. The cells were incubated with 500 μM TMRE for 30–45 min at 37°C, 5% CO₂, followed by washing once with 100 μl of HBSS containing 0.2% bovine serum albumin. A volume of 200 μL of HBSS containing 0.2% bovine serum albumin was added to each well, and the fluorescence was measured with excitation/emission : 544/590 nm.

Popova D et al., PloS One, 11(11). Fig 5. doi: 10.1371/journal.pone.0166750 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

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Flow Cytometry - TMRE-Mitochondrial Membrane Potential Assay Kit (AB113852)

Flow cytometry histogram of Jurkat cells stained with ab113852 (100nM TMRE) with (blue) or without (red) treatment with 100µM FCCP.

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PubMed

Functional Studies - TMRE-Mitochondrial Membrane Potential Assay Kit (AB113852)

TMRE assay to assess mitochondrial function after C. rodentium infection.

Maiti et al (2015) used TMRE assay kit ab113852 to assess mitochondrial membrane potential in murine distal colon after C. rodentium infection.

Image courtesy of Maiti A K et al. Sci Rep. 2015; 5: 1543. doi: 10.1038/srep15434. Reproduced under the Creative Commons License http://creativecommons.org/licenses/by/4.0/

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PubMed

Functional Studies - TMRE-Mitochondrial Membrane Potential Assay Kit (AB113852)

Mitochondrial membrane potential assayed using TMRE in the study of VIP.

Maiti AK et al (2018) used TMRE assay kit ab113852 to measure mitochondrial membrane potential in an in vitro mouse intestinal model treated with cytokines in the presence and absence of VIP (vasoactive intestinal peptide). VIP was induced by C. rodentium infection and cytokines.

Image courtesy of Maiti A K et al. PLoS One. 2018; 13(9): e0204567. doi: 10.1371/journal.pone.0204567