组织type Plasminogen活化剂Activity Assay试剂盒(Colorimetric,人) (ab108905)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Plasma, Serum
- Sensitivity: 0.13 IU/ml
概述
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产品名称
组织type Plasminogen活化剂Activity Assay试剂盒(Colorimetric,人)
参阅全部 Tissue Plasminogen Activator 试剂盒 -
检测方法
Colorimetric -
样品类型
Serum, Plasma -
检测类型
Enzyme activity (quantitative) -
灵敏度
0.13 IU/ml -
范围
0.156 IU/ml - 40 IU/ml -
检测时间
1h 00m -
种属反应性
与反应: Human -
产品概述
Tissue type Plasminogen Activator Activity Assay Kit (Colorimetric, Human) ab108905 is used to measure Human Tissue type Plasminogen Activator activity in cell culture supernatants and plasma.
The tPA assay protocol measures the ability of Tissue type Plasminogen Activator to activate the plasminogen to plasmin in coupled or indirect assays that contain Tissue type Plasminogen Activator, plasminogen, and a plasmin-specific synthetic substrate. The amount of plasmin produced is quantitated using a highly specific plasmin substrate releasing a yellow para-nitroaniline (pNA) chromophore. The change in absorbance of the pNA in the reaction solution at 405 nm is directly proportional to the Tissue type Plasminogen Activator enzymatic activity.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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说明
Previously called Tissue type Plasminogen Activator Human Chromogenic Activity Assay Kit.
Tissue type Plasminogen Activator (tPA) is a 68 kDa serine protease that converts the zymogen plasminogen into the active serine protease plasmin which digests fibrin and induces the dissolution of fibrin clots. Tissue type Plasminogen Activator is synthesized by endothelial cells in normal blood vessels and displays relatively high affinity for fibrin, suggesting that it functions predominately in physiological thrombolysis in vivo.
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平台
Microplate reader
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 100 tests 1X Diluent 1 x 30ml Human Plasminogen 3 vials Microplate 1 96 well polystyrene microplate (12 strips of 8 wells) 1 unit Plasmin Substrate 2 units Sealing Tapes 3 units Tissue type Plasminogen Activator Standard 1 unit -
研究领域
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功能
Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. Play a direct role in facilitating neuronal migration. -
组织特异性
Synthesized in numerous tissues (including tumors) and secreted into most extracellular body fluids, such as plasma, uterine fluid, saliva, gingival crevicular fluid, tears, seminal fluid, and milk. -
疾病相关
Note=Increased activity of TPA results in increased fibrinolysis of fibrin blood clots that is associated with excessive bleeding. Defective release of TPA results in hypofibrinolysis that can lead to thrombosis or embolism. -
序列相似性
Belongs to the peptidase S1 family.
Contains 1 EGF-like domain.
Contains 1 fibronectin type-I domain.
Contains 2 kringle domains.
Contains 1 peptidase S1 domain. -
结构域
Both FN1 and one of the kringle domains are required for binding to fibrin.
Both FN1 and EGF-like domains are important for binding to LRP1.
The FN1 domain mediates binding to annexin A2.
The second kringle domain is implicated in binding to cytokeratin-8 and to the endothelial cell surface binding site. -
翻译后修饰
The single chain, almost fully active enzyme, can be further processed into a two-chain fully active form by a cleavage after Arg-310 catalyzed by plasmin, tissue kallikrein or factor Xa.
Differential cell-specific N-linked glycosylation gives rise to two glycoforms, type I (glycosylated at Asn-219) and type II (not glycosylated at Asn-219). The single chain type I glycoform is less readily converted into the two-chain form by plasmin, and the two-chain type I glycoform has a lower activity than the two-chain type II glycoform in the presence of fibrin.
N-glycosylation of Asn-152; the bound oligomannosidic glycan is involved in the interaction with the mannose receptor.
Characterization of O-linked glycan was studied in Bowes melanoma cell line. -
细胞定位
Secreted > extracellular space. - Information by UniProt
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别名
- Alteplase
- DKFZp686I03148
- Plasminogen activator tissue
see all -
数据库链接
- Entrez Gene: 5327 Human
- Omim: 173370 Human
- SwissProt: P00750 Human
- Unigene: 491582 Human
图片
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Functional Studies- Tissue Type Plasminogen Activator Human ELISA Kit (ab108905)
tPA measured in biological fluids showing activity (IU) per mL of tested sample
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Functional Studies- Tissue Type Plasminogen Activator Human ELISA Kit (ab108905)Time course of tPA measured in biological fluids showing optical densities
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Functional Studies- Tissue Type Plasminogen Activator Human ELISA Kit (ab108905)Standard curve (colorimetric) : mean of duplicates (+/-SD) with background readings subtracted
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Functional Studies- Tissue Type Plasminogen Activator Human ELISA Kit (ab108905)Change of absorbance per min. of various concentrations of tPA standard
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Functional Studies- Tissue Type Plasminogen Activator Human ELISA Kit (ab108905)O.D's of various dilutions of human plasma after 20hrs using ab108905
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Functional Studies - Tissue type Plasminogen Activator Human ELISA Kit (ab108905)Tissue type Plasminogen Activator Chromogenic Activity Standard curve
数据表及文件
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SDS download
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Datasheet download
文献 (12)
ab108905 被引用在 12 文献中.
- Lee RD et al. Repurposing the KCa3.1 Blocker Senicapoc for Ischemic Stroke. Transl Stroke Res 15:518-532 (2024). PubMed: 37088858
- Lee RD et al. Immunocytoprotection after reperfusion with Kv1.3 inhibitors has an extended treatment window for ischemic stroke. Front Pharmacol 14:1190476 (2023). PubMed: 37180699
- András IE et al. Extracellular Vesicle-Serpine-1 Affects Neural Progenitor Cell Mitochondrial Networks and Synaptic Density: Modulation by Amyloid Beta and HIV-1. Mol Neurobiol 60:6441-6465 (2023). PubMed: 37458985
- Zhang Z et al. Plasma tissue-type plasminogen activator is associated with lipoprotein(a) and clinical outcomes in hospitalized patients with COVID-19. Res Pract Thromb Haemost 7:102164 (2023). PubMed: 37680312
- Al-Qahtani SM et al. The association between Annexin A2 and epithelial cell adhesion molecule in breast cancer cells. Cancer Rep (Hoboken) 5:e1498 (2022). PubMed: 34240826