AB108906

组织Factor Activity Assay试剂盒(Human，Colorimetric)

Name: Tissue Factor Activity检测试剂盒 (Human, Colorimetric) (ab108906)| Abcam中文官网
Brand: Abcam Limited
SKU: AB108906
Price: 9074 CNY
Availability: InStock

Tissue Factor Activity Assay Kit (Human, Colorimetric)

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(20 Publications)

Tissue Factor Activity Assay Kit (Human, Colorimetric) ab108906 is used to determine Human Tissue Factor activity in plasma, serum, urine, tissue and cell lysate samples.

查看别名

CD142, Tissue factor, TF, Coagulation factor III, Thromboplastin, F3

4 Images

Functional Studies - Tissue Factor Activity Assay Kit (Human, Colorimetric) (AB108906)

FuncS

Lab

Functional Studies - Tissue Factor Activity Assay Kit (Human, Colorimetric) (AB108906)

Functional studies - Tissue Factor Human Chromogenic Activity Assay Kit.

Standard curve showing concentration (pM) on X-axis and increase of O.D. per min on Y-axis (time frame : 20 min.)

FuncS

Lab

Functional Studies - Tissue Factor Activity Assay Kit (Human, Colorimetric) (AB108906)

Functional studies - Tissue Factor Human Chromogenic Activity Assay Kit.

Tissue Factor measured in various biologicals showing average concentrations

FuncS

Lab

Functional Studies - Tissue Factor Activity Assay Kit (Human, Colorimetric) (AB108906)

Functional studies - Tissue Factor Human Chromogenic Activity Assay Kit.

Colorimetric standard curve : mean of duplicates (+/- SD)

FuncS

Lab

Functional Studies - Tissue Factor Activity Assay Kit (Human, Colorimetric) (AB108906)

Functional studies - Tissue Factor Human Chromogenic Activity Assay Kit.

Time course showing O.D.'s of one standard point and one sample (undiluted vs.diluted)

关键信息

检测方法

Colorimetric

样品类型

Urine, Plasma, Tissue, Serum, Cell Lysate

反应种属

Human

检测类型

Enzyme activity (quantitative)

灵敏度

= 3.5 pmol/L

范围

7.81 - 250 pmol/L

检测时间

检测平台

Microplate reader

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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产品详情

Tissue Factor Activity Assay Kit (Human, Colorimetric) ab108906 is used to determine Human Tissue Factor activity in plasma, serum, urine, tissue and cell lysate samples.

The tissue factor activity assay protocol measures the ability of lipoprotein TF/FVIIa to activate factor X (FX) to factor Xa. The amidolytic activity of the TF/FVIIa complex is quantitated by the amount of FXa produced using a highly specific FXa substrate releasing a yellow para-nitroaniline (pNA) chromophore. The change in absorbance of the pNA at 405 nm is directly proportional to the TF enzymatic activity.

Tissue factor activity assay protocol summary:
- add reaction mix to wells
- add standards and samples
- incubate for 30 min
- add FXa substrate
- analyze with a microplate reader every 5 min for 25 min

Other Notes
Previously called Tissue Factor Human Chromogenic Activity Assay Kit.

The transmembrane protein Tissue factor (TF) is the physiologic trigger of coagulation in normal hemostasis. Tissue factor binds and allosterically activates factor VII (FVII). The TF-FVIIa complex cleaves factor IX and X, leading to thrombin generation. Tissue factor markedly enhances the ability of FVIIa to cleave both macromolecule and small peptidyl substrates.

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规格

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性能和储存信息

运输条件

Blue Ice

储存信息

Please refer to protocols

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Tissue Factor also known as coagulation factor III or CD142 is an important player in the blood coagulation process. This protein with a molecular mass of approximately 47 kDa is a transmembrane glycoprotein. It is expressed widely in various tissues including the lungs heart and brain and plays an important role in initiating the coagulation cascade when exposed to blood. In endothelial cells tissue factor is generally not present unless these cells are in a state of disturbance supporting its role as a primary defense mechanism against vascular injury.

Biological function summary

Tissue factor acts as a cofactor for factor VIIa forming a complex that rapidly catalyzes the activation of factor X to factor Xa. This catalytic action ultimately leads to fibrin formation which is essential for clotting. Tissue Factor (TF) is part of the extrinsic pathway of the coagulation cascade and is fundamental in the generation of a hemostatic response to tissue injury. By regulating thrombin generation through its interaction with other coagulation factors tissue factor plays an important role in maintaining hemostatic balance.

Pathways

Tissue factor is integral in the extrinsic pathway of coagulation which works closely with the intrinsic pathway to ensure effective hemostasis. It complexes with factor VIIa and triggers downstream effects involving proteins like factor X and thrombin. Tissue factor forms a bridge between cellular injury responses and the blood coagulation system linking these two significant physiological processes. The interaction with these proteins highlights its central role in hemostatic pathways and highlights its significance in the coagulation network.

Tissue factor is associated with conditions like thrombosis and cardiovascular diseases where its expression can become dysregulated. Abnormal activation of tissue factor contributes directly to the pathological clot formation seen in thrombosis. It is also implicated in cancer-related coagulopathy where it can facilitate tumor-associated thrombosis by interacting with cancer cell surfaces. Additionally in such diseases the interplay between tissue factor and thrombin becomes important as it affects disease progression and the severity of clot-related complications.

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产品实验方案

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靶点信息

Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF : VIIa] complex activates factors IX or X by specific limited proteolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.

See full target information F3

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文献 (20)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:10754 PubMed39737992

2024

SARS-CoV-2 infection of human pluripotent stem cell-derived vascular cells reveals smooth muscle cells as key mediators of vascular pathology during infection.

Applications

Unspecified application

Species

Unspecified reactive species

Alexsia Richards,Andrew S Khalil,Max Friesen,Troy W Whitfield,Xinlei Gao,Tenzin Lungjangwa,Roger D Kamm,Zhengpeng Wan,Lee Gehrke,David Mooney,Rudolf Jaenisch

International journal of molecular sciences 25: PubMed39201690

2024

ShSPI Inhibits Thrombosis Formation and Ischemic Stroke In Vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Ning Luan,Han Cao,Yunfei Wang,Haihao Zhang,Kangyang Lin,Jingping Hu,Mingqiang Rong,Cunbao Liu

Clinical immunology (Orlando, Fla.) 247:109240 PubMed36693535

2023

Down-regulation of KLF2 in lung fibroblasts is linked with COVID-19 immunofibrosis and restored by combined inhibition of NETs, JAK-1/2 and IL-6 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Akrivi Chrysanthopoulou,Christina Antoniadou,Anastasia-Maria Natsi,Efstratios Gavriilidis,Vasileios Papadopoulos,Evangelia Xingi,Stylianos Didaskalou,Dimitrios Mikroulis,Victoria Tsironidou,Konstantinos Kambas,Maria Koffa,Panagiotis Skendros,Konstantinos Ritis

Scientific reports 12:22191 PubMed36564503

2022

Coagulation potential and the integrated omics of extracellular vesicles from COVID-19 positive patient plasma.

Applications

Unspecified application

Species

Unspecified reactive species

Saini Setua,Kiruphagaran Thangaraju,Monika Dzieciatkowska,Rebecca B Wilkerson,Travis Nemkov,Derek R Lamb,Yutaka Tagaya,Tori Boyer,Tobi Rowden,Allan Doctor,Angelo D'Alessandro,Paul W Buehler

European journal of nutrition 62:1231-1238 PubMed36495341

2022

Dietary fat intake is associated with insulin resistance and an adverse vascular profile in patients with T1D: a pooled analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Noppadol Kietsiriroje,Hanya Shah,Marios Zare,Lauren L O'Mahoney,Daniel J West,Sam M Pearson,Ramzi A Ajjan,Matthew D Campbell

Frontiers in endocrinology 13:1008329 PubMed36213278

2022

Plasma tissue factor coagulation activity in post-acute myocardial infarction patients.

Applications

Unspecified application

Species

Unspecified reactive species

Xiong Chang Lim,Siti Maryam J M Yatim,Suet Yen Chong,Xiaoyuan Wang,Sock Hwee Tan,Xiaoxun Yang,Siew Pang Chan,A Mark Richards,Chris J Charles,Mark Y Chan,Jiong-Wei Wang

Frontiers in immunology 13:999871 PubMed36172348

2022

Manipulation of tissue factor-mediated basal PAR-2 signalling on macrophages determines sensitivity for IFNγ responsiveness and significantly modifies the phenotype of murine DTH.

Applications

Unspecified application

Species

Unspecified reactive species

Hannah Wilkinson,Hugh Leonard,Michael G Robson,Richard Smith,ElLi Tam,John H McVey,Daniel Kirckhofer,Daxin Chen,Anthony Dorling

Science translational medicine 14:eabj7465 PubMed36044595

2022

A targetable pathway in neutrophils mitigates both arterial and venous thrombosis.

Applications

Unspecified application

Species

Unspecified reactive species

Lalitha Nayak,David R Sweet,Asha Thomas,Stephanie D Lapping,Kenneth Kalikasingh,Annmarie Madera,Vinesh Vinayachandran,Roshan Padmanabhan,Neelakantan T Vasudevan,Jay T Myers,Alex Y Huang,Alvin Schmaier,Nigel Mackman,Xudong Liao,Andrei Maiseyeu,Mukesh K Jain

Diabetes & vascular disease research 19:14791641221103217 PubMed35657731

2022

Glucose variability is associated with an adverse vascular profile but only in the presence of insulin resistance in individuals with type 1 diabetes: An observational study.

Applications

Unspecified application

Species

Unspecified reactive species

Noppadol Kietsiriroje,Sam M Pearson,Lauren L O'Mahoney,Daniel J West,Robert As Ariëns,Ramzi A Ajjan,Matthew D Campbell

JCI insight 6: PubMed34324440

2021

Angiotensin II triggers release of neutrophil extracellular traps, linking thromboinflammation with essential hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Akrivi Chrysanthopoulou,Eugenia Gkaliagkousi,Antonios Lazaridis,Stella Arelaki,Panagiotis Pateinakis,Maria Ntinopoulou,Alexandros Mitsios,Christina Antoniadou,Christos Argyriou,George S Georgiadis,Vasileios Papadopoulos,Alexandra Giatromanolaki,Konstantinos Ritis,Panagiotis Skendros

View all publications

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websiteProtocolBooklet

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组织Factor Activity Assay试剂盒(Human，Colorimetric)

Tissue Factor Activity Assay Kit (Human, Colorimetric)

Functional Studies - Tissue Factor Activity Assay Kit (Human, Colorimetric) (AB108906)

Functional Studies - Tissue Factor Activity Assay Kit (Human, Colorimetric) (AB108906)

Functional Studies - Tissue Factor Activity Assay Kit (Human, Colorimetric) (AB108906)

Functional Studies - Tissue Factor Activity Assay Kit (Human, Colorimetric) (AB108906)

关键信息

检测方法

样品类型

反应种属

检测类型

灵敏度

范围

检测时间

检测平台