Soluble Collagen Assay试剂盒

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Functional Studies - Soluble Collagen Assay Kit (AB241015)

Collagen I Standard curve.

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Functional Studies - Soluble Collagen Assay Kit (AB241015)

Estimation of acid-soluble collagen content in rat tissues.

Rat leg muscle, lung and heart samples were homogenized in 0.5 M acetic acid, incubated overnight at 4C to solubilize collagen and neutralized with 0.5 M NaOH. Collagen levels (calculated as μg collagen/mg wet tissue) for the samples were : 3.83 ± 0.47 μg/mg for muscle, 1.47 ± 0.22 μg/mg for lung and 2.85 ± 0.08 μg/mg for heart.

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Functional Studies - Soluble Collagen Assay Kit (AB241015)

Estimation of secreted soluble collagen in cultured cell growth medium (DMEM/F12 medium).

3T3-L1 fibroblasts were grown to ~80% confluence in T-75 flasks and then treated with either vehicle or TGF-β (100 ng/ml), a cytokine known to stimulate synthesis and secretion of tropocollagen fibrils. After 48 hours of treatment, the culture medium was removed, centrifuged to remove debris and was assayed directly (each 10 μl clarified medium, undiluted). TGF-β treatment resulted in a roughly 1.5- fold increase in collagen secretion. Data are mean ± SEM of 3 replicates, assayed according to the kit protocol.

• Schematic Diagram

Supplier Data

Schematic Diagram - Soluble Collagen Assay Kit (AB241015)

Representative image of Soluble Collagen Assay Kit ab241015

Components shown from left to right :

- Developer Solution IX

- Peptide Labelling Reagent

- Collagen I Standard

- Collagenase Enzyme Mix

- Detection Reaction Buffer

- Collagen Assay Buffer

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.