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AB83375

Sialic Acid (NANA) Assay试剂盒

Sialic Acid (NANA) Assay Kit

5

(1 Review)

|

(12 Publications)

Sialic Acid (NANA) Assay Kit ab83375 is a simple and convenient kit for measuring free sialic acid (mainly N-acetylneuraminic acid (NANA)) in biological samples.
3 Images
Functional Studies - Sialic Acid (NANA) Assay Kit (AB83375)
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Supplier Data

Functional Studies - Sialic Acid (NANA) Assay Kit (AB83375)

Examples of colorimetric and fluorometric standard Sialic acid curves using ab83375.

Functional Studies - Sialic Acid (NANA) Assay Kit (AB83375)
  • FuncS

Unknown

Functional Studies - Sialic Acid (NANA) Assay Kit (AB83375)

Concentrations of free SA decreased (P < 0.0001), whereas bound and total SA increased (P < 0.0001) across lactation. Measured using ab83375.

Mudd, Austin T et al., Frontiers in nutrition: vol. 3 39., Fig 3, doi:10.3389/fnut.2016.00039

Functional Studies - Sialic Acid (NANA) Assay Kit (AB83375)
  • FuncS

Lab

Functional Studies - Sialic Acid (NANA) Assay Kit (AB83375)

Standard curve (fluorimetric) : mean of duplicates (+/-SD) with background readings subtrated

关键信息

检测方法

Colorimetric/Fluorometric

样品类型

Urine, Cell culture media, Cell culture supernatant

灵敏度

= 1 µM

范围

0.1 - 10 µM

检测时间

40m

检测平台

Microplate reader

产品详情

Sialic Acid (NANA) Assay Kit (ab83375) is a simple and convenient kit for measuring free sialic acid (mainly N-acetylneuraminic acid (NANA)) in biological samples.

The sialic acid assay uses an enzyme coupled reaction in which oxidation of free sialic acid creates an intermediate that reacts stoichiometrically with the probe to generate a product that can detected by absorbance (OD = 570 nm) or fluorescence (Ex/Em=535/587 nm).

The kit measures sialic acid in the linear range of 0.1 to 10 nmol with a detection sensitivity ~1 uM concentration.

Other Notes
This product was previously called K566 Biovision Sialic Acid (NANA) Colorimetric/Fluorometric Assay Kit. Biovision was acquired by Abcam in 2021.

Sialic acid is a generic term for the N- or O-substituted derivatives of neuraminic acid, a monosaccharide with a nine-carbon backbone. It is also the name for the most common member of this group, N-acetylneuraminic acid (NANA). Sialic acids are found widely distributed in animal tissues and to a lesser extent in other species ranging from plants and fungi to yeasts and bacteria, mostly in glycoproteins and gangliosides. It has been shown recently that sialic acid level may be associated with developmental and pathological stages.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

规格

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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
-20°C
储存信息
-20°C

产品实验方案

文献 (12)

Recent publications for all applications. Explore the full list and refine your search

The Journal of clinical investigation 134: PubMed39436703

2024

Sialylated glycoproteins suppress immune cell killing by binding to Siglec-7 and Siglec-9 in prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ru M Wen,Jessica C Stark,G Edward W Marti,Zenghua Fan,Aram Lyu,Fernando Jose Garcia Marques,Xiangyue Zhang,Nicholas M Riley,Sarah M Totten,Abel Bermudez,Rosalie Nolley,Hongjuan Zhao,Lawrence Fong,Edgar G Engleman,Sharon J Pitteri,Carolyn R Bertozzi,James D Brooks

Nature communications 14:8135 PubMed38065959

2023

Airway environment drives the selection of quorum sensing mutants and promote Staphylococcus aureus chronic lifestyle.

Applications

Unspecified application

Species

Unspecified reactive species

Xiongqi Ding,Catherine Robbe-Masselot,Xiali Fu,Renaud Léonard,Benjamin Marsac,Charlene J G Dauriat,Agathe Lepissier,Héloïse Rytter,Elodie Ramond,Marion Dupuis,Daniel Euphrasie,Iharilalao Dubail,Cécile Schimmich,Xiaoquan Qin,Jessica Parraga,Maria Leite-de-Moraes,Agnes Ferroni,Benoit Chassaing,Isabelle Sermet-Gaudelus,Alain Charbit,Mathieu Coureuil,Anne Jamet

Nature communications 14:1293 PubMed36894557

2023

N-acetylneuraminic acid links immune exhaustion and accelerated memory deficit in diet-induced obese Alzheimer's disease mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Stefano Suzzi,Tommaso Croese,Adi Ravid,Or Gold,Abbe R Clark,Sedi Medina,Daniel Kitsberg,Miriam Adam,Katherine A Vernon,Eva Kohnert,Inbar Shapira,Sergey Malitsky,Maxim Itkin,Alexander Brandis,Tevie Mehlman,Tomer M Salame,Sarah P Colaiuta,Liora Cahalon,Michal Slyper,Anna Greka,Naomi Habib,Michal Schwartz

Molecular psychiatry 27:1405-1415 PubMed35260802

2022

The schizophrenia-associated variant in SLC39A8 alters protein glycosylation in the mouse brain.

Applications

Unspecified application

Species

Unspecified reactive species

Robert G Mealer,Sarah E Williams,Maxence Noel,Bo Yang,Alexandria K D'Souza,Toru Nakata,Daniel B Graham,Elizabeth A Creasey,Murat Cetinbas,Ruslan I Sadreyev,Edward M Scolnick,Christina M Woo,Jordan W Smoller,Ramnik J Xavier,Richard D Cummings

Pathogens (Basel, Switzerland) 10: PubMed34832557

2021

Analysis of Extracellular Vesicles Surface Glycans Reveals Potential Immune Evasion Mechanism and New Insights on Their Origins of Biogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Maude Dagenais,Jared Q Gerlach,George R Wendt,James J Collins,Louise E Atkinson,Angela Mousley,Timothy G Geary,Thavy Long

Transfusion and apheresis science : official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis 60:103039 PubMed33388248

2021

N-acetylcysteine reduce the stress induced by cold storage of platelets: A potential way to extend shelf life of platelets.

Applications

Unspecified application

Species

Unspecified reactive species

Mallikarjun Handigund,Jeong Tae Kim,Tae Won Bae,Jaehyeon Lee,Yong Gon Cho

International journal of chronic obstructive pulmo 15:311-321 PubMed32103930

2020

Circulating Biomarkers of Handgrip Strength and Lung Function in Chronic Obstructive Pulmonary Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Rizwan Qaisar,Asima Karim,Tahir Muhammad

Colloids and surfaces. B, Biointerfaces 187:110614 PubMed31753616

2019

Charged glycan residues critically contribute to the adsorption and lubricity of mucins.

Applications

Unspecified application

Species

Unspecified reactive species

Matthias Marczynski,Bizan N Balzer,Kun Jiang,Theresa M Lutz,Thomas Crouzier,Oliver Lieleg

Cell death & disease 9:416 PubMed29549328

2018

Nuclear galectin-1-FOXP3 interaction dampens the tumor-suppressive properties of FOXP3 in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Gao,Xiaoju Li,Zhen Shu,Kuo Zhang,Xiaochang Xue,Weina Li,Qiang Hao,Zhaowei Wang,Wangqian Zhang,Shuning Wang,Cheng Zeng,Dong Fan,Wei Zhang,Yingqi Zhang,Huadong Zhao,Meng Li,Cun Zhang

PloS one 12:e0182683 PubMed28787006

2017

Addition of αGal HyperAcute™ technology to recombinant avian influenza vaccines induces strong low-dose antibody responses.

Applications

Unspecified application

Species

Unspecified reactive species

Wenlan Alex Chen,Jinjin Zhang,Katie M Hall,Carol B Martin,Serguei Kisselev,Emily J Dasen,Nicholas N Vahanian,Charles J Link,Brian K Martin
View all publications
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