SEAP Reporter Gene Assay试剂盒(Luminescence) (ab133077)
Key features and details
- Assay type: Quantitative
- Detection method: Luminescent
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Cell culture media
概述
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产品名称
SEAP Reporter Gene Assay试剂盒(Luminescence) -
检测方法
Luminescent -
样品类型
Cell culture media -
检测类型
Quantitative -
检测时间
1h 00m -
种属反应性
与反应: Mammals, Other species -
产品概述
SEAP Reporter Gene Assay Kit (Luminescence) (ab133077) provides a simple chemiluminescence method for the sensitive quantitation of SEAP (secreted alkaline phosphatase) in conditioned cell culture medium from transfected cells. The assay can detect SEAP activity in the microunits/well (mU/ml) range. The kit includes enough reagents to run three 96-well plates. The assay is easy to perform and can be completed within one hour.
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说明
Secreted alkaline phosphatase (SEAP) is commonly used as a reporter of gene expression. Compared to other conventional intracellular reporters such as chloramphenicol acetyltransferase (CAT) and firefly luciferase, SEAP has the advantage of being secreted from transfected cells into the culture medium. SEAP activity in the culture medium is directly proportional to changes in intracellular concentrations of SEAP mRNA and protein. In addition, the kinetics of gene expression can be studied using the same cultures by repeatedly collecting culture medium at different time points. The intact cells can be used for further analysis of RNA or protein expression.
SEAP activity was first measured using the chromogenic alkaline phosphatase substrate p-nitrophenyl phosphate (pNPP). Today, the most sensitive SEAP assays employ chemiluminescent alkaline phosphatase substrates such as the 1,2-dioxetane CSPD. Chemiluminescent detection of SEAP is fast, easy to perform, and sensitive.
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平台
Microplate reader
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 300 tests 300 tests 96-Well Solid Plate (white) with lid 3 units 3 units Cell-Based Alkaline Phosphatase Standard 1 x 200µl 1 x 200µl SEAP Substrate (Luminescence) 1 x 15ml 1 x 15ml -
研究领域
图片
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab133077 被引用在 3 文献中.
- Galvan S et al. Regulation of Transgene Expression by the Natural Sweetener Xylose. Adv Sci (Weinh) 9:e2203193 (2022). PubMed: 36316222
- Wang L et al. PI3K/AKT phosphorylation activates ERRa by upregulating PGC-1a and PGC-1ß in gallbladder cancer. Mol Med Rep 24:N/A (2021). PubMed: 34184087
- Lee H et al. R-spondins are BMP receptor antagonists in Xenopus early embryonic development. Nat Commun 11:5570 (2020). PubMed: 33149137