Propidium Iodide (ab14083)
概述
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产品名称
Propidium Iodide -
产品概述
Propidium Iodide (PI) binds to double-stranded DNA. PI cannot cross intact plasma membrane and therefore will only be present in DNA of cells where the plasma membrane has been compromised/ permeabilized.
For Microscopy analysis: PI can be viewed using rhodamine(red) filter (λ= 536/617). Cells will only be stained if the membrane has been permeated, either naturally (non-viable cells) or with detergents (for fluorescent staining).
For Flow Cytometry analysis: PI staining can be monitored in FL2 channel.
If used together as control for Annexin V assays ab14082, ab14083 or ab14152, PI should be diluted to 250 µg/ml solution (in PBS) prior use and added as 1 µl/ Annexin V Assay (0.25µg/assay).
Visit our FAQs page for tips and troubleshooting.
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说明
This product is manufactured by BioVision, an Abcam company and was previously called 1056 Propidium Iodide. 1056-1 is the same size as the 1 ml size of ab14083.
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经测试应用
适用于: FM, Flow Cytmore details
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 标识符 1 ml Propidium Iodide (1mg/ml) ab14083 1 x 1ml -
研究领域
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相关性
Propidium Iodide (PI) (MW=668.4 Da) is an intercalating agent and a fluorescent molecule which is membrane impermeant and generally excluded from viable cells. Upon entering cells, PI will bind to DNA and RNA by intercalating between bases. Once bound to the nucleic acids, its fluorescence is enhanced 20- to 30-fold. Exmax= 536nm / Emmax= 617 nm.
相关产品
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应用
The Abpromise guarantee
Abpromise™承诺保证使用ab14083于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
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| FM |
Use at an assay dependent concentration.
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| Flow Cyt |
Use at an assay dependent concentration.
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| 说明 |
|---|
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FM
Use at an assay dependent concentration. |
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Flow Cyt
Use at an assay dependent concentration. |
图片
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Flow cytometry: ab14083Image from Wang et al., PLoS One., 10(9). Fig 3a & c. doi:10.1371/journal.pone.0137712 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Wang et al. used ab14083 to investigate an optimized platform for maintaining proliferation of giant panda mesenchymal stem cells (MSCs). MSCs were fixed in 70% alcohol and 30% PBS at 4°C for 1 hour. Cells are then incubated in the dark with PBS, 20µg/ml propidium iodide (ab14083) and 1% RNaseA for 30 minutes at 37°C. Cell samples are resuspended in PBS and analyzed using FACS Calibur flow cytometry.
Flow cytometry analysis shows the cells are at different cell cycle stages in different concentrations (0ng/ml and 5ng/ml respectively) of basic fibroblast growth factor (bFGF).
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Immunofluorescence analysis using ab14083Image from Melia MM et al ., Plos One.,29 (8). Fig 1b DOI: 10.1371/journal.pone.0106281. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Vero-DogSLAM (VDS) cells and Vero (African Green Monkey kidney) cells were infected with wtCDV. Cells were fixed, permeabilised and stained with SSPE serum and rabbit anti-human FITC; nuclei were stained using ab14083 propidium iodide. Images were taken using a Nikon Eclipse TE2000-U UV microscope (x400).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (51)
ab14083 被引用在 51 文献中.
- Wu W et al. Treatment with quercetin inhibits SARS-CoV-2 N protein-induced acute kidney injury by blocking Smad3-dependent G1 cell-cycle arrest. Mol Ther 31:344-361 (2023). PubMed: 36514292
- Liu Z et al. SUMO1 regulates post-infarct cardiac repair based on cellular heterogeneity. J Pharm Anal 13:170-186 (2023). PubMed: 36908856
- Aylan B et al. ATG7 and ATG14 restrict cytosolic and phagosomal Mycobacterium tuberculosis replication in human macrophages. Nat Microbiol 8:803-818 (2023). PubMed: 36959508
- Yang J et al. Downregulation of HAS‑2 regulates the chondrocyte cytoskeleton and induces cartilage degeneration by activating the RhoA/ROCK signaling pathway. Int J Mol Med 52:N/A (2023). PubMed: 37232339
- Amirova KM et al. The Triterpenoid Nrf2 Activator, CDDO-Me, Decreases Neutrophil Senescence in a Murine Model of Joint Damage. Int J Mol Sci 24:N/A (2023). PubMed: 37240121
