AB139437

PKC Kinase Activity Assay试剂盒

Name: PKC Kinase Activity检测试剂盒 (ab139437)| Abcam中文官网
Brand: Abcam Limited
SKU: AB139437
Price: 10579 CNY
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PKC Kinase Activity Assay Kit

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(37 Publications)

4 Images

Functional Studies - PKC Kinase Activity Assay Kit (AB139437)

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Functional Studies - PKC Kinase Activity Assay Kit (AB139437)

1.5 x e7 THP-1 cells were incubated with 100 nM GF109203X (GF; ab144264) or Ro31-8220 mesylate (Ro; ab120374) for 30 minutes prior to activation with 10 μg x mL^-1 PMA (Sigma) for 4 hours. Control cells were left without inhibitors or PMA. Cells were lysed in 1 mL of lysis buffer, and 30 μL were tested for PKC activity (duplicates; +/- SD).

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Functional Studies - PKC Kinase Activity Assay Kit (AB139437)

Signal from active PKC with background signal subtracted (duplicates; +/- SD).

FuncS

Lab

Functional Studies - PKC Kinase Activity Assay Kit (AB139437)

Titration of ab139437 (duplicates; +/- SD).

Schematic Diagram - PKC Kinase Activity Assay Kit (AB139437)

Schematic Diagram

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Schematic Diagram - PKC Kinase Activity Assay Kit (AB139437)

schematic diagram of the assay.

Fitzpatrick, Daniel P. (2020) "A Mechanistic Study on the Non-genotoxic Carcinogenicity of the Food Contaminant Semicarbazide," Sure-J : Science Undergraduate Research Journal : Vol. 2 : Iss. 1, Article 2.
Available at : https : //arrow.tudublin.ie/sure_j/vol2/iss1/2

This image is courtesy of Fitzpatrick, Daniel P. (2020) "A Mechanistic Study on the Non-genotoxic Carcinogenicity of the Food Contaminant Semicarbazide," Sure-J: Science Undergraduate Research Journal: Vol. 2: Iss. 1, Article 2

关键信息

检测方法

Colorimetric

样品类型

Purified protein, Suspension cells, Tissue Extracts, Adherent cells

检测类型

Enzyme activity

检测时间

4h 30m

检测平台

Microplate reader

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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产品详情

PKC Kinase Activity Assay Kit (ab139437) is a non-radioactive assay providing a safe, rapid and reliable method for the screening of inhibitors or activators of PKC and for quantitating the activity of PKC in purified or partially purified enzyme preparations. This kit is based on a solid phase enzyme-linked immuno-absorbent assay (ELISA) that utilizes a specific synthetic peptide as a substrate for PKC and a polyclonal antibody that recognizes the phosphorylated form of the substrate.

PKA activity assay principle
In this assay, a synthetic peptide is used as PKC specific substrate. The substrate, which has pre-coated on the wells on the microplate well provided, is phosphorylated by the PKC present in the sample after addition of ATP.

The phosphorylated substrate is recognized by a polyclonal antibody that recognizes only the phospho-substrate. The phospho-specific antibody is subsequently recognized a HRPconjugated secondary antibody.

The assay is finally developed with TMB substrate and color develops in proportion to the PKC activityexisting in the sample. The color development is stopped with acid stop solution and the intensity of the color is measured in a microplate reader at OD 450 nm.

The assay is designed for the analysis of PKC activity in the solution phase. For the measurement of PKC in partially purified, purified, or crude enzyme preparations from any species.

The kit offers the following advantages:
1. Safe - non-radioactive measurement of kinase activity.
2. Flexible - kinetic and end-point options available.
3. Fast - results in < 4.5 hours.
4. Efficient - only 30 μl diluted sample needed per well.

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精确度

[ { "reproducibilityType": "Intra", "sample": "Sample", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Sample", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]

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规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab139437-1x96Tests", "assetComponentDetails": [ { "size":"1 x 30 mL", "name":"20X Wash Buffer", "number":"AB139437-CMP08", "productcode":"" }, { "size":"1 x 5 mL", "name":"PKC Phosphospecific Substrate Antibody", "number":"AB139437-CMP05", "productcode":"" }, { "size":"1 x 10 mL", "name":"Antibody Dilution Buffer", "number":"AB139437-CMP01", "productcode":"" }, { "size":"1 x 20 µL", "name":"Anti-Rabbit IgG: HRP Conjugate", "number":"AB139437-CMP02", "productcode":"" }, { "size":"1 x 2 mg", "name":"ATP", "number":"AB139437-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"PKC Substrate Microtiter Plate", "number":"AB139437-CMP06", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Substrate", "number":"AB139437-CMP09", "productcode":"" }, { "size":"1 x 10 mL", "name":"Stop Solution 2", "number":"AB139437-CMP07", "productcode":"" }, { "size":"1 x 30 µL", "name":"Active PKC", "number":"AB139437-CMP10", "productcode":"" }, { "size":"1 x 10 mL", "name":"Kinase Assay Dilution Buffer", "number":"AB139437-CMP04", "productcode":"" } ] } } }

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性能和储存信息

运输条件

Dry Ice

储存信息

Please refer to protocols

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Protein kinase C (PKC) comprises a family of serine/threonine kinases with several isoforms such as PKC alpha beta and gamma among others. PKC enzymes play key roles in intracellular signaling particularly in mediating responses to growth factors hormones and other signals. PKC exhibits a molecular weight generally ranging around 77 to 97 kDa depending on the isoform. These enzymes show expression in many tissues but have high concentrations in the brain heart and lung. PKC activation often involves translocation to cellular membranes which is important for their signaling roles.

Biological function summary

PKC influences various cellular processes such as cell proliferation differentiation apoptosis and immune responses. It acts within larger protein complexes serving as a modulator of cellular functions. The activation of PKC leads to its association with different scaffolding proteins impacting a wide array of cell activities. PKC notably affects the function of other kinases and transcription factors demonstrating its integral role in managing cellular behavior and homeostasis.

Pathways

PKC participates in the phosphoinositide signaling pathway and the mitogen-activated protein kinase (MAPK) pathway. In these contexts PKC activation impacts proteins like Ras and Raf kinases which play roles in cellular growth and differentiation. By modulating these pathways PKC becomes an important component in signal transduction cascades influencing cell fate decisions. Understanding the interaction between PKC and these pathways helps in elucidating its role in maintaining normal cellular activities and responses to external stimuli.

PKC is linked to cancer and cardiovascular diseases. In cancer dysregulation of PKC activity often contributes to tumor growth and progression by altering pathways that control cell division and survival. PKC abnormally interacts with proteins like Bcl-2 promoting cell survival. In cardiovascular diseases changes in PKC signaling can impact heart function contributing to conditions like heart failure through interactions with proteins such as troponin. Research on PKC and these diseases can provide insights into developing targeted therapies.

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产品实验方案

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靶点信息

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文献 (37)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed38203337

2024

Mechanistic Investigation of WWOX Function in NF-kB-Induced Skin Inflammation in Psoriasis.

Applications

Unspecified application

Species

Unspecified reactive species

Min-Jeong Shin,Hyun-Sun Kim,Pyeongan Lee,Na-Gyeong Yang,Jae-Yun Kim,Yun-Su Eun,Whiin Lee,Doyeon Kim,Young Lee,Kyung-Eun Jung,Dongkyun Hong,Jung-Min Shin,Sul-Hee Lee,Sung-Yul Lee,Chang-Deok Kim,Jung-Eun Kim

Biomedicines 11: PubMed37239160

2023

Decreased MARCKS Protein Expression in Kidney Cortex Membrane Fractions of Cathepsin B Knockout Mice Is Associated with Reduced Lysophosphatidylcholine and Protein Kinase C Activity.

Applications

Unspecified application

Species

Unspecified reactive species

Tamim Kawakibi,Niharika Bala,Lauren P Liu,Louis A Searcy,Nancy D Denslow,Abdel A Alli

Nature communications 14:2728 PubMed37169746

2023

Monoterpenoid aryl hydrocarbon receptor allosteric antagonists protect against ultraviolet skin damage in female mice.

Applications

Unspecified application

Species

Unspecified reactive species

Karolína Ondrová,Iveta Zůvalová,Barbora Vyhlídalová,Kristýna Krasulová,Eva Miková,Radim Vrzal,Petr Nádvorník,Binod Nepal,Sandhya Kortagere,Martina Kopečná,David Kopečný,Marek Šebela,Fraydoon Rastinejad,Hua Pu,Miroslav Soural,Katharina Maria Rolfes,Thomas Haarmann-Stemmann,Hao Li,Sridhar Mani,Zdeněk Dvořák

BMC biology 21:52 PubMed36882743

2023

Temperature-induced embryonic diapause in chickens is mediated by PKC-NF-κB-IRF1 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Junxiao Ren,Zhengzheng Hu,Quanlin Li,Shuang Gu,Fangren Lan,Xiqiong Wang,Jianbo Li,Junying Li,Liwa Shao,Ning Yang,Congjiao Sun

Environmental science and pollution research international 30:47742-47754 PubMed36745351

2023

Sublethal biochemical, behavioral, and physiological toxicity of extremely low dose of bendiocarb insecticide in Periplaneta americana (Blattodea: Blattidae).

Applications

Unspecified application

Species

Unspecified reactive species

Milena Jankowska,Bartosz Augustyn,Justyna Maliszewska,Barbara Przeździecka,Dawid Kubiak,Olga Chełchowska,Jagoda Kaczorek,Dominik Knop,Kornelia Krajnik,Hanna Kletkiewicz,Jacek Kęsy,Justyna Rogalska,Maria Stankiewicz

European journal of histochemistry : EJH 66: PubMed36305269

2022

VEGF promotes diabetic retinopathy by upregulating the PKC/ET/NF-κB/ICAM-1 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Meiying Zhang,Min Zhou,Xia Cai,Yan Zhou,Xueling Jiang,Yan Luo,Yue Hu,Rong Qiu,Yanrong Wu,Yuejin Zhang,Yan Xiong

Redox biology 52:102309 PubMed35395625

2022

Mechano-signaling via Piezo1 prevents activation and p53-mediated senescence of muscle stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yundong Peng,Jingjing Du,Stefan Günther,Xinyue Guo,Shengpeng Wang,Andre Schneider,Li Zhu,Thomas Braun

Molecules (Basel, Switzerland) 26: PubMed34833857

2021

Understanding the Mechanism of Action of NAI-112, a Lanthipeptide with Potent Antinociceptive Activity.

Applications

Unspecified application

Species

Unspecified reactive species

Arianna Tocchetti,Marianna Iorio,Zeeshan Hamid,Andrea Armirotti,Angelo Reggiani,Stefano Donadio

Science translational medicine 13: PubMed34321319

2021

An ACE inhibitor reduces bactericidal activity of human neutrophils in vitro and impairs mouse neutrophil activity in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Duo-Yao Cao,Jorge F Giani,Luciana C Veiras,Ellen A Bernstein,Derick Okwan-Duodu,Faizan Ahmed,Catherine Bresee,Warren G Tourtellotte,S Ananth Karumanchi,Kenneth E Bernstein,Zakir Khan

American journal of physiology. Cell physiology 321:C535-C548 PubMed34288724

2021

Increased endothelial sodium channel activity by extracellular vesicles in human aortic endothelial cells: putative role of MLP1 and bioactive lipids.

Applications

Unspecified application

Species

Unspecified reactive species

Mohammad-Zaman Nouri,Ling Yu,Lauren P Liu,Kevin M Chacko,Nancy D Denslow,John F LaDisa,Abdel A Alli

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PKC Kinase Activity Assay试剂盒

PKC Kinase Activity Assay Kit

Functional Studies - PKC Kinase Activity Assay Kit (AB139437)

Functional Studies - PKC Kinase Activity Assay Kit (AB139437)

Functional Studies - PKC Kinase Activity Assay Kit (AB139437)

Schematic Diagram - PKC Kinase Activity Assay Kit (AB139437)