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Phosphatidic Acid Assay试剂盒(Fluorometric) (ab273335)

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Example data
  • Example data
  • Example data

Key features and details

  • Assay type: Quantitative
  • Detection method: Fluorescent
  • Platform: Microplate
  • Sample type: Cell culture extracts, Tissue Extracts

概述

  • 产品名称

    Phosphatidic Acid Assay试剂盒(Fluorometric)

  • 检测方法

    Fluorescent

  • 样品类型

    Cell culture extracts, Tissue Extracts

  • 检测类型

    Quantitative

  • 实验步骤

    Multiple steps standard assay

  • 产品概述

    Phosphatidic Acid Assay Kit (Fluorometric) (ab273335) is a plate-based enzymatic assay for quantitation of Phosphatidic Acid (PA) in cells and tissues.
    The PA Converter hydrolyzes PA to form an intermediate which, in the presence of a developer and enzyme mix, converts a non-fluorescent probe to a fluorescent product (Ex/Em= 535/587 nm) that can be quantified. The Converter is specific to PA and does not hydrolyze other phospholipids (e.g. those with more complex head groups such as phosphatidylcholine or cardiolipin), allowing direct quantitation of PA.
    This kit can detect as low as 40 pmol PA per well.

  • 说明

    This product is manufactured by BioVision, an Abcam company and was previously called K748 PicoProbeTM Phosphatidic Acid Assay Kit (Fluorometric). K748-100 is the same size as the 100 test size of ab273335.

    The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the SDS download section.

  • 平台

    Microplate

性能

  • 存放说明

    Store at -20°C. Please refer to protocols.
  • 组件 100 tests
    Development Enzyme Mix VI 1 vial
    Development Enzyme Mix VII 1 vial
    PA Assay Buffer 1 x 100ml
    PA Converter 1 vial
    PA Standard 1 x 100µl
    PicoProbe I 1 x 400µl

图片

  • Example data
    Example data

    Example data.

    Phosphatidic acid standard curve.

  • Example data
    Example data

    Example data.

    Phosphatidic acid content in rat liver and HEK 293 cells. For rat liver extract, 100 mg tissue was homogenized in 1 ml PA assay buffer. Lipid extraction was carried out and was re-suspended in 200 µl 5% Triton X-100 solution. For HEK293, cells were grown to confluency and harvested using a rubber policeman.

  • Example data
    Example data

    Example data.

    Assay Specificity: Equimolar amounts (20 nmol) of phosphatidic acid (PA), phosphatidylcholine (PC), phosphatidylinositol (PI) and cardiolipin (CL) show interference less than 20% with respect of Phosphatidic Acid. Assay shows specificity for phosphatidic acid.

文献 (1)

发表研究结果有使用 ab273335？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab273335 被引用在 1 文献中.

  • Kang H  et al. DGKB mediates radioresistance by regulating DGAT1-dependent lipotoxicity in glioblastoma. Cell Rep Med 4:100880 (2023). PubMed: 36603576

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