Phenylalanine Assay试剂盒
Phenylalanine Assay Kit (Fluorometric)
Be the first to review this product! Submit a review
|
(1 Publication)
- FuncS
Lab
Functional Studies - Phenylalanine Assay Kit (Fluorometric) (AB83376)
Phenylalanine fluorimetric standard curve : mean of duplicates (+/- SD) with background readings subtracted
- FuncS
Lab
Functional Studies - Phenylalanine Assay Kit (Fluorometric) (AB83376)
Phenylalanine measured in cell lysates showing quantities (micromoles) per million of cells tested.
- FuncS
Lab
Functional Studies - Phenylalanine Assay Kit (Fluorometric) (AB83376)
Phenylalanine measured in biofluids showing concentration in micromolar.
产品详情
Phenylalanine Assay Kit ab83376 provides a quick, simple, accurate method for quantifying phenylalanine in biological samples.
Phenylalanine assay protocol
In the phenylalanine assay protocol, phenylalanine is reductively deaminated with the simultaneous formation of NADH which reacts with our fluorescent probe to generate fluorescence at Ex/Em=535/587 nm. The assay is linear in the range from 0.1 to 1.0 nmol (2-20 μM) of Phenylalanine.
Phenylalanine assay protocol summary
- add samples and standards to wells
- add reaction mix and incubate for 60 min
- analyze with a microplate reader
Other Notes
This product was previously called K572 Biovision Phenylalanine Fluorometric Assay Kit. Biovision was acquired by Abcam in 2021.
L-Phenylalanine (PHE) is an electrically-neutral amino acid, one of the twenty common and one of the three aromatic amino acids used to biochemically form proteins. Phenylalanine uses the same active transport channel as tryptophan to cross the blood-brain barrier, and, in large quantities, interferes with the production of serotonin.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
规格
性能和储存信息
运输条件
推荐的短期储存条件
推荐的长期储存条件
储存信息
补充信息
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Phenylalanine aids in the formation of proteins by incorporating into the polypeptide chains during ribosomal translation. It is not part of a molecular complex but acts as a free amino acid in various cellular processes. Its conversion to tyrosine by the enzyme phenylalanine hydroxylase is an important step in its metabolism. This conversion is necessary because tyrosine is used in the production of neurotransmitters and hormones.
Pathways
Phenylalanine plays an important role in the phenylalanine and tyrosine catabolic pathway. This pathway is critical for the metabolism of aromatic amino acids eventually leading to the synthesis of important compounds like dopamine and norepinephrine. The enzyme phenylalanine hydroxylase closely related to this pathway shows the connection between phenylalanine and subsequent tyrosine-derived neurotransmitters illustrating its importance in neurochemical regulation.
文献 (1)
Recent publications for all applications. Explore the full list and refine your search
Lasers in surgery and medicine 55:490-502 PubMed37051852
2023
Applications
Unspecified application
Species
Unspecified reactive species
搭配使用产品
Abcam Product Promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com