Phalloidin-iFluor 555试剂(ab176756)
Key features and details
- Assay type: Cell-based (qualitative)
- Platform: Fluorescence microscope
- Sample type: Adherent cells, Suspension cells
概述
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产品名称
Phalloidin-iFluor 555试剂
参阅全部 F-actin 试剂盒 -
样品类型
Adherent cells, Suspension cells -
检测类型
Cell-based (qualitative) -
产品概述
Phalloidin-iFluor 555 Reagent (ab176756) is one of a series of phalloidin conjugates that bind to actin filaments, also known as F-actin. Phallodin-iFluor 555 can be detected with a fluorescent microscope at Ex/Em = 556/574 nm.
Phalloidin conjugates are convenient probes for labeling, identifying and quantifying animal or plant actin filaments in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. They can also be used in paraffin-embedded samples that have been de-paraffinized.
Review other popular phalloidin dye conjugates, including Phalloidin-iFluor 488, Phalloidin-iFluor 647, Phalloidin-iFluor 594, and Rhodamine Phalloidin, search the website to see all phallodin conjugates, or read the phalloidin staining protocol.
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说明
Staining fixed cell or tissue samples with phalloidin conjugates is very simple; it requires a single 20-90 min incubation with the phalloidin, followed by 3 short wash steps. Phalloidin staining can be combined with antibody-based staining by adding the phalloidin conjugate during either the primary or secondary antibody incubation step.
When used in unfixed samples, phalloidin binding leads to a decrease in the disassociation rate of actin subunits from the ends of actin filaments, essentially stabilizing actin filaments through the prevention of filament depolymerisation.
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平台
Fluorescence microscope
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 300 tests Phalloidin-iFluor 555 Conjugate 1 x 300 tests -
研究领域
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别名
- actin filament
- f actin
- Filamentous actin
图片
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Functional Studies - Phalloidin-iFluor 555 Reagent (ab176756)Wong, Michael K et al., PloS one?vol. 13,6 e0199632., Fig 6, doi:10.1371/journal.pone.0199632Immunofluorescent images of Phalloidin staining at day 3 across various surface thicknesses. Red fluorescence indicates phalloidin staining for F-actin and blue fluorescence indicates DAPI staining for cell nuclei.
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CytoPainter Phalloidin-iFluor 555 Reagent (ab176756)Actin filaments staining in HeLa cells. Actin filaments (red) were stained with CytoPainter Phalloidin-iFluor 555 reagent (ab176755). Nuclei were stained with Nuclear Green DCS1 (ab138905).
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CytoPainter Phalloidin-iFluor 555 Reagent (ab176756)Excitation and emission of phalloidin-iFluor 555 reagent.
数据表及文件
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SDS download
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Datasheet download
文献 (88)
ab176756 被引用在 88 文献中.
- Song E et al. Electrospun Nanofiber Membrane for Cultured Corneal Endothelial Cell Transplantation. Bioengineering (Basel) 11:N/A (2024). PubMed: 38247931
- Ueda Y et al. Rap1 organizes lymphocyte front-back polarity via RhoA signaling and talin1. iScience 26:107292 (2023). PubMed: 37520697
- Yen CH et al. pH-driven continuous stem cell production with enhanced regenerative capacity from polyamide/chitosan surfaces. Mater Today Bio 18:100514 (2023). PubMed: 36524151
- Qian J et al. Toll-like receptor-2 in cardiomyocytes and macrophages mediates isoproterenol-induced cardiac inflammation and remodeling. FASEB J 37:e22740 (2023). PubMed: 36583707
- Takeda T et al. Identification of active spermatogenesis using a multiphoton microscope. Andrology 11:1147-1156 (2023). PubMed: 36597184