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Phalloidin-iFluor 350试剂(ab176751)

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CytoPainter Phalloidin-iFluor 350 Reagent (ab176751)
  • CytoPainter Phalloidin-iFluor 350 Reagent (ab176751)

Key features and details

  • Assay type: Cell-based (qualitative)
  • Platform: Fluorescence microscope
  • Sample type: Adherent cells, Suspension cells

概述

  • 产品名称

    Phalloidin-iFluor 350试剂
    参阅全部 F-actin 试剂盒

  • 样品类型

    Adherent cells, Suspension cells

  • 检测类型

    Cell-based (qualitative)

  • 产品概述

    Phalloidin-iFluor 350 Reagent (ab176751) is one of a series of phalloidin conjugates that bind to actin filaments, also known as F-actin. Phalloidin-iFluor 350 can be easily detected with a fluorescent microscope at Ex/Em = 353/442 nm.


    Phalloidin conjugates are convenient probes for labeling, identifying and quantifying animal or plant actin filaments in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. They can also be used in paraffin-embedded samples that have been de-paraffinized.


    Review other popular phalloidin dye conjugates, including Phalloidin-iFluor 488 (ab176753), Phalloidin-iFluor 647 (ab176759), Phalloidin-iFluor 594 (ab176757), Phalloidin-iFluor 555 (ab176756), and Rhodamine Phalloidin (ab235138), search the website to see all phallodin conjugates, or read the phalloidin staining protocol.

  • 说明

    Staining fixed cell or tissue samples with phalloidin conjugates is very simple; it requires a single 20-90 min incubation with the phalloidin, followed by 3 short wash steps. Phalloidin staining can be combined with antibody-based staining by adding the phalloidin conjugate during either the primary or secondary antibody incubation step.

    When used in unfixed samples, phalloidin binding leads to a decrease in the disassociation rate of actin subunits from the ends of actin filaments, essentially stabilizing actin filaments through the prevention of filament depolymerisation.

  • 平台

    Fluorescence microscope

性能

图片

  • CytoPainter Phalloidin-iFluor 350 Reagent (ab176751)
    CytoPainter Phalloidin-iFluor 350 Reagent (ab176751)

    Actin filaments staining in HeLa cells. Actin filaments (blue) were stained with CytoPainter Phalloidin-iFluor 350 reagent (ab176751).

  • CytoPainter Phalloidin-iFluor 350 Reagent (ab176751)
    CytoPainter Phalloidin-iFluor 350 Reagent (ab176751)

    Excitation and emission spectra of phalloidin-iFluor 350 reagent.

文献 (0)

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Staining for actin in mouse embryonic fibroblasts

 Good Excellent 5/5 (Ease of Use)
Abreviews
abreview image
The product worked well for mouse embryonic fibroblasts at a dilution of 1:100 and an incubation time of 30 mins. This review is submitted as required by the terms of discount code #96248
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MS. XXXXXX XXXXXX

Verified customer

提交于 Dec 08 2014

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