Peroxidase Activity Assay试剂盒

产品详情

Peroxidase Assay Kit ab155895 provides a convenient colorimetric and fluorometric means to measure the peroxidase activity in biological samples. In the presence of Peroxidase, the OxiRed Probe reacts with H₂O₂ in a 1:1 stoichiometry to produce the red fluorescent oxidation product, resorufin. The resorufin is quantified by colorimetric (570nm) or fluorometric methods (Ex/Em = 535/587 nm). The assay is simple, direct, highly sensitive and high throughput-ready. The detection limit is 0.1 mU per well via colorimetric or 0.01 mU per well via fluorometric method, based on our unit definition.

Other Notes
This product was previously called K772 Peroxidase Activity Colorimetric/Fluorometric Assay Kit. Biovision was acquired by Abcam in 2021.

Peroxidases (EC number 1.11.1.x) are a large family of enzymes that typically catalyze a reaction of the form: ROOR' + electron donor (2 e-) + 2H+ to ROH + R'OH. For many of these enzymes the optimal substrate is hydrogen peroxide, but others are more active with organic hydroperoxides such as lipid peroxides. Peroxidases can contain a heme cofactor in their active sites, or alternately redox-active cysteine or selenocysteine residues.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.