PAF Acetylhydrolase抑制剂Screening Assay试剂盒(ab133091)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
概述
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产品名称
PAF Acetylhydrolase抑制剂Screening Assay试剂盒
参阅全部 PAF Acetylhydrolase 试剂盒 -
检测方法
Colorimetric -
检测类型
Enzyme activity -
产品概述
ab133091 uses 2-thio PAF as a substrate for PAF Acetylhydrolase (PAF-AH). Upon hydrolysis of the acetyl thioester bond at the sn-2 position by PAF Acetylhydrolase, free thiols are detected using 5,5’-dithio-bis-(2-nitrobenzoic acid) (DTNB). The PAF Acetylhydrolase Inhibitor Screening assay includes Human plasma PAF Acetylhydrolase as control and is a time saving tool for screening vast numbers of inhibitors.
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平台
Microplate reader
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 96 tests 2-thio PAF (substrate) 2 vials 96-Well Plate (Colorimetric Assay) 1 unit 96-Well Plate Cover 1 unit Human Plasma PAF-AH 2 vials PAF-AH Assay Buffer (10X) 1 vial PAF-AH Assay DTNB 4 vials -
研究领域
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功能
Exhibits lysophospholipase, transacylase, PAF acetylhydrolase and asparaginase activities. -
序列相似性
In the N-terminal section; belongs to the asparaginase 1 family.
Contains 5 ANK repeats. - Information by UniProt
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别名
- 60 kDa lysophospholipase
- A530050D06Rik
- AI429460
see all
图片
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Functional Studies - PAF Acetylhydrolase Inhibitor Screening Assay Kit (ab133091)Inhibition of Human plasma PAF Acetylhydrolase by MAFP (IC50 = 250 nM).
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab133091 被引用在 1 文献中.
- Toyama MH et al. Gallic Acid as a Non-Selective Inhibitor of a/ß-Hydrolase Fold Enzymes Involved in the Inflammatory Process: The Two Sides of the Same Coin. Pharmaceutics 14:N/A (2022). PubMed: 35214100