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AB242302

Oxidized LDL Assay试剂盒(MDA-LDL,人)

Oxidized LDL Assay Kit (MDA-LDL, Human)

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(3 Publications)

Oxidized LDL Assay Kit (MDA-LDL, Human) ELISA Kit (ab242302) is developed for the detection and quantitation of oxidized phospholipids (OxPL) associated with human LDL in plasma, serum or other biological fluid samples.
2 Images
ELISA - Oxidized LDL Assay Kit (MDA-LDL, Human) (AB242302)
  • ELISA

Supplier Data

ELISA - Oxidized LDL Assay Kit (MDA-LDL, Human) (AB242302)

Human OxLDL Standard Curve

ELISA - Oxidized LDL Assay Kit (MDA-LDL, Human) (AB242302)
  • ELISA

Supplier Data

ELISA - Oxidized LDL Assay Kit (MDA-LDL, Human) (AB242302)

Quantitation of OxLDL in Serum and Plasma Samples.

Left : LDL Recovery After Precipitation Solution. Serum and plasma samples were treated with LDL Precipitation Solution according to the Sample Preparation protocol. LDL recovery was determined. Right : OxLDL Determination of Serum and Plasma Samples. Serum and plasma samples were treated with LDL Precipitation Solution according to the Sample Preparation Section. Precipitated LDL pellets were resuspended in 1.6 mL of PBS before further diluting 1 : 160 in Assay Diluent. Samples were tested according to the Assay Protocol.

关键信息

检测方法

Colorimetric

样品类型

Plasma, Serum

反应种属

Human

灵敏度

= 50 ng/mL

检测平台

Microplate

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搭配使用产品

Assay Kits

AB118970

Lipid Peroxidation (MDA) Assay Kit (Colorimetric/Fluorometric)

Biochemical assay - Lipid Peroxidation (MDA) Assay Kit (Colorimetric/Fluorometric) (AB118970)

  • 5
  • 3
Primary Antibodies

AB125066

Anti-Glutathione Peroxidase 4 antibody [EPNCIR144]

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (AB125066)

  • 5
  • 13
Assay Kits

AB238538

Lipid Peroxidation (4-HNE) Assay Kit

Competitive ELISA - Lipid Peroxidation (4-HNE) Assay Kit (AB238538)

  • 0
  • 0
Reagents, Controls & Accessories

AB110413

Total OXPHOS Rodent WB Antibody Cocktail

Western blot - Total OXPHOS Rodent WB Antibody Cocktail (AB110413)

  • 5
  • 18
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

Oxidized LDL Assay Kit (MDA-LDL, Human) ELISA Kit (ab242302) is developed for the detection and quantitation of oxidized phospholipids (OxPL) associated with human LDL in plasma, serum or other biological fluid samples.

The kit contains an OxLDL Standard and has a detection sensitivity limit of <15 ng/mL. Each kit provides sufficient reagents to perform up to 96 assays including standard curve and unknown samples.

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规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab242302-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB242302-CMP08", "productcode":"" }, { "size":"1 x 100 mL", "name":"10X Wash Buffer", "number":"AB242302-CMP01", "productcode":"" }, { "size":"1 x 50 mL", "name":"Assay Diluent", "number":"AB242302-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"LDL Precipitation Solution (2X)", "number":"AB242302-CMP06", "productcode":"" }, { "size":"1 x 200 µL", "name":"Blocking Reagent (100X)", "number":"AB242302-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"Substrate Solution", "number":"AB242302-CMP10", "productcode":"" }, { "size":"1 x 25 µL", "name":"OxLDL Standard", "number":"AB242302-CMP07", "productcode":"" }, { "size":"1 x 20 µL", "name":"Streptavidin-Enzyme Conjugate", "number":"AB242302-CMP09", "productcode":"" }, { "size":"1 x 20 µL", "name":"Biotinylated Anti-Human ApoB-100 Antibody (1000X)", "number":"AB242302-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-MDA Antibody Coated Plate", "number":"AB242302-CMP02", "productcode":"" } ] } } }
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
Multi
推荐的长期储存条件
Multi
储存信息
Please refer to protocols
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Oxidized phospholipids often referred to as oxidized LDL or ox-LDL play a role in lipid metabolism and atherosclerosis development. Ox-LDL results from the oxidative modification of low-density lipoprotein (LDL) molecules heavily impacting cardiovascular health. The major component of ox-LDL malondialdehyde-modified LDL (MDA-LDL) expresses on the surface of endothelial cells macrophages and smooth muscle cells. This oxidative process increases the atherogenic properties of LDL contributing to its accumulation in arterial walls.
Biological function summary

Oxidized phospholipids strongly influence inflammation and immune responses. These molecules act within cell membranes triggering toll-like receptors (TLRs) and other immune sensors. Ox-LDL forms part of immunoglobulin complexes and interacts with scavenger receptors like CD36 on macrophages. It recruits immune cells and promotes the release of cytokines perpetuating an inflammatory environment within blood vessels.

Pathways

Oxidized phospholipids participate in lipid oxidative stress and inflammatory pathways. They contribute significantly to the pathophysiology of the atherosclerotic process. In the oxidative stress pathways ox-LDL affects the balance of reactive oxygen species (ROS) and nitric oxide (NO) levels influencing vasodilation and endothelial function. It engages proteins such as apolipoprotein B-100 in the inflammatory process and connects to pathways involving NF-kB influencing various cellular responses.

Oxidized phospholipids relate mainly to cardiovascular diseases and atherosclerosis. The accumulation and interaction of ox-LDL with cells in the arterial wall drive plaque formation and instability which can lead to myocardial infarction. Additionally these modified lipids link with diabetes-related complications due to their role in promoting vascular inflammation. Proteins like lectin-like oxidized LDL receptor-1 (LOX-1) mediate ox-LDL uptake emphasizing its damaging role in metabolic syndromes.
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产品实验方案

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靶点信息

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

International journal of biological sciences 21:1275-1293 PubMed39897035

2025

Exosomal mediated lipid metabolism, ferritinophagy and CoQ-dependent pathway contributes to the ferroptosis of keratinocyte in SJS/TEN.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Zhang,Pei Qiao,ChunYing Xiao,ZiPeng Cao,JiaoLing Chen,Hui Fang,JianKang Yang,ZeHua Kang,ErLe Dang,Shuai Shao,BingYu Pang,QingYang Li,ZhenLai Zhu,ShengXian Shen,Akito Hasegawa,Riichiro Abe,HongJiang Qiao,Gang Wang,Meng Fu

Molecular medicine reports 25: PubMed35211757

2022

Advanced glycation end products promote osteoporosis by inducing ferroptosis in osteoblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Weiwei Ge,Jian Jie,Jie Yao,Wei Li,Yahui Cheng,Wenjuan Lu

Experimental and therapeutic medicine 21:250 PubMed33603858

2021

Correlations of cardiac function with inflammation, oxidative stress and anemia in patients with uremia.

Applications

Unspecified application

Species

Unspecified reactive species

Heping Zhang,Lei Fan,Huawei Liao,Lirong Tu,Jie Zhang,Dan Xu,Jiangchao Feng
View all publications
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websiteProtocolBooklet
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