NQO1 Activity Assay试剂盒(ab184867)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 30 min
- Sample type: Adherent cells, Cell culture extracts, Cell Lysate, Suspension cells, Tissue Extracts, Tissue Homogenate, Tissue Lysate
概述
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产品名称
NQO1 Activity Assay试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 3 2.183% 批次间 样品 n Mean SD CV% Overall 9 6.035% -
样品类型
Cell culture extracts, Adherent cells, Suspension cells, Tissue Extracts, Cell Lysate, Tissue Homogenate, Tissue Lysate -
检测类型
Enzyme activity -
检测时间
0h 30m -
种属反应性
与反应: Mouse, Rat, Cow, Human -
产品概述
NQO1 Activity Assay kit (ab184867) is designed for the sensitive and accurate measurement of NQO1 (NAD(P)H dehydrogenase [quinone] 1) activity.
The enzyme activity is determined by following the reduction of Menadione with cofactor NADH and the simultaneous reduction of WST1 which leads to increased absorbance at 440 nm. Dicoumarol is used as an inhibitor of NQO1 as part of the assay.
NQO1 assay protocol summary:
- add samples to wells
- add reaction buffer + inhibitor and reaction buffer to separate sample wells
- analyze with a microplate reader for 5 min -
说明
NQO1 serves as a quinone reductase in connection with conjugation reactions of hydroquinones involved in detoxification pathways, NQO1 protects against quinone-induced damage by competing with potentially toxic one-electron pathways. It also functions in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.
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平台
Microplate reader
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 1000X Cofactor (lyophilized) 1 vial 1000X Inhibitor (lyophilized) 1 vial 100X Dye (lyophilized) 1 vial 20X Basic Buffer 1 x 3ml 2X Extraction Buffer 1 x 15ml 5000X Menadione 1 x 100µl 500X NADH (lyophilized) 1 vial 96-well Microplate 2 units -
研究领域
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细胞定位
Cytoplasmic -
别名
- Azoreductase
- DIA4
- DT-diaphorase
see all -
数据库链接
- Entrez Gene: 519632 Cow
- Entrez Gene: 1728 Human
- Entrez Gene: 18104 Mouse
- Entrez Gene: 24314 Rat
- SwissProt: P15559 Human
- SwissProt: Q64669 Mouse
- SwissProt: P05982 Rat
相关产品
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Positive Controls
- Bovine Heart Mitochondria (ab110338)
- Rat liver tissue lysate - mitochondrial extract (ab110346)
- Rat heart tissue lysate - mitochondrial extract (ab110347)
- Rat brain tissue lysate - mitochondrial extract (ab110348)
- Mouse liver tissue lysate - mitochondrial extract (ab110349)
- Mouse heart tissue lysate - mitochondrial extract (ab110350)
- Mouse brain tissue lysate - mitochondrial extract (ab110351)
图片
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An example is shown below where the rate/slope is calculated between these time points.
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This raw data is expressed as rate (mOD/min) per microgram of cell extract added per well as shown above. The molar extinction coefficient factor for the WST1 dye is 25.9/mM/well.
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Figure 3a
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Figure 3b
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Figure 3c
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Figure 4
数据表及文件
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SDS download
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Datasheet download
文献 (13)
ab184867 被引用在 13 文献中.
- Iori S et al. Deepening the Whole Transcriptomics of Bovine Liver Cells Exposed to AFB1: A Spotlight on Toll-like Receptor 2. Toxins (Basel) 14:N/A (2022). PubMed: 35878242
- Akhtari FS et al. High-throughput screening and genome-wide analyses of 44 anticancer drugs in the 1000 Genomes cell lines reveals an association of the NQO1 gene with the response of multiple anticancer drugs. PLoS Genet 17:e1009732 (2021). PubMed: 34437536
- Pauletto M et al. Discovering the Protective Effects of Resveratrol on Aflatoxin B1-Induced Toxicity: A Whole Transcriptomic Study in a Bovine Hepatocyte Cell Line. Antioxidants (Basel) 10:N/A (2021). PubMed: 34439473
- Liu Y et al. Fucoxanthin Pretreatment Ameliorates Visible Light-Induced Phagocytosis Disruption of RPE Cells under a Lipid-Rich Environment via the Nrf2 Pathway. Mar Drugs 20:N/A (2021). PubMed: 35049870
- Farooqui Z et al. Nrf2 inhibition induces oxidative stress, renal inflammation and hypertension in mice. Clin Exp Hypertens 43:175-180 (2021). PubMed: 33070655