AB228548

NADP Regeneration Kit

Name: NADP Regeneration Kit (ab228548)| Abcam中文官网
Brand: Abcam Limited
SKU: AB228548
Price: 4278 CNY
Availability: InStock

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NADP Regeneration Kit (ab228548) provides two ready to use solutions to regenerate NADP by a simple mixing.

关键信息

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产品详情

NADP Regeneration Kit (ab228548) provides two ready to use solutions to regenerate NADP by a simple mixing. This kit can be used for all NADP-requiring systems (e.g. 12-Ketochenodeoxycholic Acid synthesis, Ferredoxin Reductase System). About 300-500 enzyme assays can be performed using this kit. The total number of assays that can be performed depends on the experimental design.

NADP is the electron acceptor for biosynthetic reactions and dehydrogenation reactions. NADP is used for anabolic pathways, such as lipid synthesis, cholesterol synthesis and fatty acid chain elongation. It is also a necessary cofactor in many xenobiotic metabolism reactions. In chloroplasts, NADP is reduced to NADPH by ferredoxin-NADP reductase in the last step of the electron chain in photosynthesis reactions. Many oxidoreductases and all ligases use NADP/NADPH as coenzyme. NADP can also be used in the determination of amylase, creatine kinase, glucose-6-phosphate dehydrogenase, isocitrate dehydrogenase.

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规格

{ "values": { "200Test": { "sellingSize": "200 Test", "publicAssetCode":"ab228548-200Test", "assetComponentDetails": [ { "size":"1 x 10 mL", "name":"NADP Regenerating Buffer II", "number":"AB228548-CMP03", "productcode":"" }, { "size":"1 x 10 mL", "name":"NADP Regenerating Buffer I", "number":"AB228548-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"NADP Regenerating Enzyme Mix", "number":"AB228548-CMP04", "productcode":"" }, { "size":"1 x 2 Vial", "name":"NADPH", "number":"AB228548-CMP01", "productcode":"" } ] } } }

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性能和储存信息

运输条件

Blue Ice

储存信息

-20°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

NADP/NADPH also known as Nicotinamide Adenine Dinucleotide Phosphate and its reduced form plays an essential role in cellular metabolism. It is a coenzyme with a molecular mass of around 743.4 g/mol. NADP/NADPH is ubiquitously expressed in cells due to its integral role in metabolic processes. It is found in high concentrations within the cytoplasm and chloroplasts where it functions in various biochemical reactions.

Biological function summary

NADPH facilitates reductive biosynthesis and maintains cellular redox status. It acts as a major electron donor in various anabolic reactions including lipid and nucleic acid synthesis. NADPH also contributes to the detoxification of reactive oxygen species and serves in the process of oxidative phosphorylation. Its cofactor activity associates it predominantly with enzymatic complexes such as the cytochrome P450 system.

Pathways

NADPH is fundamental in both the pentose phosphate pathway and the Calvin cycle. In the pentose phosphate pathway it provides reducing power important for fatty acid and cholesterol biosynthesis. In photosynthetic organisms NADPH functions in the Calvin cycle facilitating carbon fixation. The enzyme glucose-6-phosphate dehydrogenase related to NADPH production is integral in NADPH recycling.

Impaired NADPH function links to conditions like chronic granulomatous disease and diabetes. Chronic granulomatous disease occurs due to defects in the NADPH oxidase complex impairing the body’s ability to combat pathogens effectively. Diabetes has associations with oxidative stress influenced by imbalance in NADPH levels. In these diseases NADPH's interaction with proteins like superoxide dismutase affects oxidative damage management and overall immune response.

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产品实验方案

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靶点信息

See full target information Triphosphopyridine nucleotide

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websiteProtocolBooklet

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NADP Regeneration Kit