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Myeloperoxidase (MPO) Activity Assay试剂盒(Colorimetric) (ab105136)

Reviews (3)Q&A (4)References (89)
Functional Studies - Myeloperoxidase (MPO) Activity Assay Kit (Colorimetric) (ab105136)
  • MPO activity
  • Typical Standard Curve

Key features and details

  • Assay type: Enzyme activity
  • Detection method: Colorimetric
  • Platform: Microplate reader
  • Assay time: 1 hr
  • Sample type: Cell culture supernatant, Other biological fluids, Serum, Tissue Homogenate, Urine (UTI)

概述

  • 产品名称

    Myeloperoxidase (MPO) Activity Assay试剂盒(Colorimetric)
    参阅全部 Myeloperoxidase 试剂盒

  • 检测方法

    Colorimetric

  • 样品类型

    Cell culture supernatant, Serum, Other biological fluids, Tissue Homogenate, Urine (UTI)

  • 检测类型

    Enzyme activity

  • 检测时间

    1h 00m

  • 种属反应性

    与反应: Mammals, Other species

  • 产品概述

    Myeloperoxidase (MPO) Activity Assay Kit ab105136 is a rapid, simple, sensitive, and reliable colorimetric assay suitable for use as a high throughput MPO activity assay.


    In the MPO assay protocol, myeloperoxidase produces HClO from H2O2 and Cl-. The HClO reacts with taurine to generate the taurine chloramine, which subsequently reacts with the DTNB probe to eliminate color (absorbance at 412 nm). The absorbance is inversely proportional to the amount of MPO enzyme.


    This MPO assay kit can be used to detect myeloperoxidase as low as 0.05 mU per well.


    MPO assay protocol summary:
    - add samples to wells (and assay buffer only to wells for standards)
    - add reaction mix to sample wells and incubate for 30 min to 2 hrs
    - add stop mix and incubate for 10 min
    - add TNB reagent/standard to all wells and incubate for 5-10 min
    - analyze with a microplate reader


    Technical Note: MPO is unlikely to be detectable in healthy urine but may be elevated in the presence of infection.

  • 说明

    This product is manufactured by BioVision, an Abcam company and was previously called K744 Myeloperoxidase (MPO) Colorimetric Activity Assay Kit. K744-100 is the same size as the 100 test size of ab105136.

    Myeloperoxidase (MPO) is a peroxidase enzyme (EC 1.11.1.7) most abundantly expressed in neutrophil granulocytes. It is a lysosomal protein stored in the azurophilic granules of the neutrophil. Myeloperoxidase contains a heme pigment which causes its green color in secretions rich in neutrophils, such as pus and some forms of mucus. Myeloperoxidase catalyzes the production of hypochlorous acid (HClO) from hydrogen peroxide (H2O2) and chloride anion (Cl-, or the equivalent from a non-chlorine halide). Myeloperoxidase also oxidizes tyrosine to a tyrosyl radical using hydrogen peroxide as an oxidizing agent.

    The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the SDS download section.

  • 平台

    Microplate reader

性能

相关产品

图片

  • Functional Studies - Myeloperoxidase (MPO) Activity Assay Kit (Colorimetric) (ab105136)
    Functional Studies - Myeloperoxidase (MPO) Activity Assay Kit (Colorimetric) (ab105136)Namkoong, Ho et al., PLoS pathogens?vol. 14,4 e1006955., S4 fig, doi:10.1371/journal.ppat.1006955
    MPO activity in indicated cells was measured using the commercially available MPO Activity Assay Kit (n = 3).
  • MPO activity
    MPO activity

    a. Measurement of MPO activity using WBC lysate (3 μg) and MPO Positive Control (5 and 10 μL). b. MPO specific activity in WBC lysate.

  • Typical Standard Curve
    Typical Standard Curve

    Typical standard curve for ab105136.

文献 (89)

发表研究结果有使用 ab105136？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab105136 被引用在 89 文献中.

  • Han G  et al. Oral TNF-α siRNA delivery via milk-derived exosomes for effective treatment of inflammatory bowel disease. Bioact Mater 34:138-149 (2024). PubMed: 38223538
  • Singh A  et al. Interleukin-11 Suppresses Ocular Surface Inflammation and Accelerates Wound Healing. Invest Ophthalmol Vis Sci 64:1 (2023). PubMed: 37910094
  • Rahmani AH  et al. Quercetin, a Plant Flavonol Attenuates Diabetic Complications, Renal Tissue Damage, Renal Oxidative Stress and Inflammation in Streptozotocin-Induced Diabetic Rats. Metabolites 13:N/A (2023). PubMed: 36677055
  • Tintelnot J  et al. Microbiota-derived 3-IAA influences chemotherapy efficacy in pancreatic cancer. Nature 615:168-174 (2023). PubMed: 36813961
  • Bhat TA  et al. Not all vaping is the same: differential pulmonary effects of vaping cannabidiol versus nicotine. Thorax 78:922-932 (2023). PubMed: 36823163
View all Publications for this product

客户评价及客户问答

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1-7 of 7 Abreviews or Q&A

MPO Measurement in Mice Colon Tissue When Induced with DSS

 Good Good 4/5 (Ease of Use)
Abreviews
abreview image
We used the protocol in order to perform our assay. For every 10mg of tissue we used 400uL of MPO Assay Buffer in order to extract out samples. We then did a titration plate with samples undiluted, 1:5, 1:50 and 1:100, for different times points 30min, 60min and 120min. After reading and doing the calculation, most samples were falling right on the border of the highest point on the standard curve. Once we spoke with the Abcam team, they told us that dilutions should not be higher than 1:10. Since we did not test that dilution, our only possible interpretation would be based on the 1:5 dilutions at the different time points. In that case, for our samples, the best protocol was 1:5 dilution for 1 hour incubation. Please see attached picture.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Bruno Lemos

Verified customer

提交于 Jul 31 2018

MPO activity measurement in Mouse tissues (lung, kidney, spleen)

 Good Good 4/5 (Ease of Use)
Abreviews
I used a different protocol for sample preparation of tissues of mouse origin,. Using the supplied protocol resulted in formation of insoluble material in several dilutions
1) 30 mg of frozen tissue was utilized
2) tissue was once washed in 500 µl PBS followed by centrifugation at 13.000 g for 5 min at 4°C
3) tissue was homogenized in 250 µl PBS containing 10mM NEM (N-Ethylmaleimide) and homogenized with a micro tissue lyzer
4) centrifugation at 13000 g at 4°C for 20 min and the supernatant was removed
5) 250 µl PBS containing 0,5% HTA-Br (w/v) was added
6) Samples were sonicated for 30 sec on ice
7) centrifugation for 20 min at 4°C and 8000g
8) the supernatant was utilized in a dilution of 1:2 to 1:10 for the assay directly
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

FRAU DR. Katharina Rump

Verified customer

提交于 Mar 01 2016

Higer reading in sample

 Inconclusive Average 3/5 (Ease of Use)
Abreviews
abreview image
I homogenized mouse lever tissues using Bio Masher(nippi) .
First time I used Pestle motor for nippi Bio masher (Left image).
Second Bio Masher using no Pestle motor (Right image).
I can't measure samples OD by automated homoginizer.
First time OD can't be measured diluted with MPO Assay Buffer 1:1000.
Second time OD can be measured at dilution 1:30 (homoginized by hand manual).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

眞智子 平賀

Verified customer

提交于 Aug 21 2014

Question

I'd like to know the components of the MPO assay buffer please?

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Answer

The composition of the MPO assay buffer is proprietary. However we can share that it is a Citrate-based buffer containing 50 mM Taurine and 0.1% NP-40.

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Question

There is an example of background and 5 and 10 ul positive control samples on the datasheet. How long were samples run (30', 1 hr, 2 hr?) to produce this data?

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Answer

When we measure the positive controls we do it kinetically over time. Then we select a time between 30 mins to 2 hours and make the graph. It is important to note that the activity of the positive control varies from lot to lot and the essence of this positive control is to help validate that all components of the kit are working well. We do not recommend trying to exactly replicate the absolute values from the positive control data given on the datasheet.

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Question

I was looking into using the above mentioned kit for MPO measurement. I was wondering do you have any information if this method will work of frozen serum/plasma or is fresh blood always needed.

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Answer

Although it would be preferable to use fresh samples it is probably ok to use frozen serum/plasma with this kit. We ourselves have always used fresh samples with this assay however, we know of scientists who used tissue homogenates stored at -80°C for a short while before use. Since we don’t ourselves have a comparative data measuring MPO in stored vs fresh samples, I cannot comment on if and how much of efficiency of detection is lost in freezing the samples.

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Question

Probe tube was spun in a balanced microcentrifuge and the bottom broke off. Would like to receive another probe.

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Answer

Thank you very much for you call today and for letting us know about the problem with this tube of probe.

As we discussed, please send me the original order or PO number, and I'll see if we can just send you a replacement vial of the probe.

So that we can look into the issue with the probe tubes, do you remember the speed and time that the tube was spun for?

I look forward to hearing from you. If you have any questions or need anything else, please let me know and I'll be happy to help.

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