AB105136

Myeloperoxidase (MPO) Activity Assay试剂盒(Colorimetric)

Name: Myeloperoxidase (MPO) Activity检测试剂盒 (Colorimetric) (ab105136)| Abcam中文官网
Brand: Abcam Limited
SKU: AB105136
Price: 9163 CNY
Availability: InStock
Rating: 4 (3 reviews)

Myeloperoxidase (MPO) Activity Assay Kit (Colorimetric)

(3 Reviews)

(104 Publications)

Myeloperoxidase (MPO) Activity Assay Kit ab105136 is a rapid, simple, sensitive, and reliable colorimetric assay suitable for use as a high throughput MPO activity assay. Readout is on any colorimetric (412 nm) plate reader.

- Cited in > 90 publications
- Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

查看别名

Myeloperoxidase, MPO

4 Images

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Supplier Data

Functional Studies - Myeloperoxidase (MPO) Activity Assay Kit (Colorimetric) (AB105136)

a. Measurement of MPO activity using WBC lysate (3 μg) and MPO Positive Control (5 and 10 μL). b. MPO specific activity in WBC lysate.

FuncS

Supplier Data

Functional Studies - Myeloperoxidase (MPO) Activity Assay Kit (Colorimetric) (AB105136)

Typical standard curve for ab105136.

FuncS

Unknown

Functional Studies - Myeloperoxidase (MPO) Activity Assay Kit (Colorimetric) (AB105136)

MPO activity in indicated cells was measured using the commercially available MPO Activity Assay Kit (n = 3).

Namkoong, Ho et al., PLoS pathogens: vol. 14,4 e1006955., S4 fig, doi:10.1371/journal.ppat.1006955

Schematic Diagram - Myeloperoxidase (MPO) Activity Assay Kit (Colorimetric) (AB105136)

Schematic Diagram

Supplier Data

Schematic Diagram - Myeloperoxidase (MPO) Activity Assay Kit (Colorimetric) (AB105136)

Representative image of Myeloperoxidase (MPO) Activity Assay Kit (Colorimetric) ab105136

Components shown from left to right :

- DTNB Probe

- Hydrogen Peroxide Solution

- MPO Positive Control

- Stop Mix

- TCEP

- MPO Assay Buffer

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

关键信息

检测方法

Colorimetric

样品类型

Tissue Homogenate, Cell culture supernatant, Serum, Other biological fluids, Urine (UTI)

检测类型

Enzyme activity

检测时间

检测平台

Microplate reader

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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产品详情

How the assay works

In the Myeloperoxidase (MPO) Activity Assay Kit (Colorimetric) protocol, myeloperoxidase produces HClO from H2O2 and Cl-. The HClO reacts with taurine to generate the taurine chloramine, which subsequently reacts with the DTNB probe to eliminate color (absorbance at 412 nm). The absorbance is inversely proportional to the amount of MPO enzyme.

Myeloperoxidase (MPO) Activity Assay Kit (Colorimetric) protocol summary:

- Add samples to wells (and assay buffer only to wells for standards)
- Add reaction mix to sample wells and incubate for 30 min to 2 hrs
- Add stop mix and incubate for 10 min
- Add TNB reagent/standard to all wells and incubate for 5-10 min
- Analyze with a microplate reader

Technical Note: MPO is unlikely to be detectable in healthy urine but may be elevated in the presence of infection.

How other researchers are using

Myeloperoxidase (MPO) Activity Assay Kit (Colorimetric) has been used in a variety of sample type including:
HEK293T cells ¹
Mice corneal lysates ²
Mouse uterus samples ³
References:

1-Han G et al. 2023
2-Singh A et al. 2023
3-Gao Y J et al. 2023

Related and recommended products

For fluorometric detection, we recommend the following kits:
- Myeloperoxidase (MPO) Activity Assay Kit (Fluorometric) ab111749
- Myeloperoxidase (MPO) Activity Assay Kit (Fluorometric - Red) ab219925
- Myeloperoxidase (MPO) Peroxidation Activity Assay Kit (Fluorometric) ab273334

This product is manufactured by BioVision, an Abcam company and was previously called K744 Myeloperoxidase (MPO) Colorimetric Activity Assay Kit. K744-100 is the same size as the 100 test size of ab105136.

Myeloperoxidase (MPO) is a peroxidase enzyme (EC 1.11.1.7) most abundantly expressed in neutrophil granulocytes. It is a lysosomal protein stored in the azurophilic granules of the neutrophil. Myeloperoxidase contains a heme pigment which causes its green color in secretions rich in neutrophils, such as pus and some forms of mucus. Myeloperoxidase catalyzes the production of hypochlorous acid (HClO) from hydrogen peroxide (H₂O₂) and chloride anion (Cl-, or the equivalent from a non-chlorine halide). Myeloperoxidase also oxidizes tyrosine to a tyrosyl radical using hydrogen peroxide as an oxidizing agent.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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规格

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab105136-100Test", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"MPO Positive Control", "number":"AB105136-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"TCEP", "number":"AB105136-CMP06", "productcode":"" }, { "size":"1 x 1 Each", "name":"Stop Mix", "number":"AB105136-CMP04", "productcode":"" }, { "size":"1 x 50 µL", "name":"Hydrogen Peroxide Solution", "number":"AB105136-CMP03", "productcode":"" }, { "size":"1 x 50 µL", "name":"DTNB Probe", "number":"AB105136-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"MPO Assay Buffer", "number":"AB105136-CMP01", "productcode":"" } ] } } }

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性能和储存信息

运输条件

Blue Ice

储存信息

-20°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Myeloperoxidase also called MPO is an enzyme that plays a critical role in the body's immune response. This protein has a mass of approximately 150 kDa and exists as a dimer composed of heavy and light polypeptide chains. Myeloperoxidase is prominently expressed in neutrophils and monocytes which are types of white blood cells important for combating infections. The enzyme catalyzes the production of hypochlorous acid and other reactive substances by utilizing hydrogen peroxide and chloride ions. These reactive substances help in neutralizing pathogens during the immune response.

Biological function summary

The generation of reactive oxygen species by myeloperoxidase is essential for microbicidal activity. Myeloperoxidase functions as part of the antimicrobial system in the phagosome which is the intracellular compartment where pathogens are degraded. This enzyme works in conjunction with other components of the immune system such as NADPH oxidase. By generating hypochlorous acid MPO contributes to the oxidative burst a rapid release of reactive oxygen species during the response to pathogens.

Pathways

Myeloperoxidase integrates into the immune defense and inflammatory pathways. In particular it is associated with the neutrophil degranulation pathway where it releases its enzymatic contents to fight off microbes. MPO also interacts with proteins involved in oxidative stress processes such as superoxide dismutase which moderates levels of reactive oxygen species in cells. These interactions ensure balance in the immune response preventing excessive tissue damage during inflammation.

Dysregulated MPO activity can contribute to the development of diseases. For instance myeloperoxidase is linked with atherosclerosis a cardiovascular condition where inflammation and oxidative stress lead to plaque formation in the arteries. It also associates with vasculitis an autoimmune disorder causing inflammation of blood vessels. Both disorders can relate to the inflammatory pathways that involve MPO and proteins like C-reactive protein which serves as a marker of inflammation. Understanding MPO's role in these conditions is important for effective therapeutic interventions.

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产品实验方案

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靶点信息

Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity (PubMed : 9922160). Mediates the proteolytic cleavage of alpha-1-microglobulin to form t-alpha-1-microglobulin, which potently inhibits oxidation of low-density lipoprotein particles and limits vascular damage (PubMed : 25698971).

See full target information MPO

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文献 (104)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pharmacology 16:1546010 PubMed40606616

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Innovative pH-responsive alginate-coated rosuvastatin-loaded chitosan nanoparticles: a targeted approach to inhibiting HMGB1-activated RAGE/TLR4-NFκB signaling in colonic inflammation in rats.

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Elsayed A Elmorsy,Sameh Saber,Zubida M Al-Majdoub,Rabab S Hamad,Mustafa Ahmed Abdel-Reheim,Asmaa Ramadan,Norah Suliman Alsoqih,Mariam S Alharbi,Hamad Alsaykhan,Alshaimaa A Farrag,Hanan Eissa,Rasha Abdelhady,Abousree T Ellethy,Mostafa M Khodeir,Hossam A Elsisi,Syed Suhail Ahmed,Ahmed Y Kira

Current research in pharmacology and drug discovery 8:100223 PubMed40492109

2025

Gallic acid serves as an effective therapeutic agent of inflammatory bowel disease: Pharmacological impacts on tight junction-dependent intestinal permeability and its related intracellular signaling.

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Apiwan Arinno,Pichayapa Sukmak,Purisha Kulworasreth,Thaniya Sricharunrat,Chutima S Vaddhanaphuti,Pawin Pongkorpsakol

NPJ Parkinson's disease 11:94 PubMed40287421

2025

Association between serum myeloperoxidase enzyme activity and Parkinson's disease status.

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Emilio Fernández Espejo,María-Del-Mar Guerra,Silvia Castellano

International journal of molecular sciences 25: PubMed39684207

2024

Endothelial Dysfunction and Impaired Wound Healing Following Radiation Combined Skin Wound Injury.

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Li Wang,Bin Lin,Min Zhai,Lisa Hull,Wanchang Cui,Mang Xiao

PloS one 19:e0311310 PubMed39570897

2024

Development of a novel complex inflammatory bowel disease mouse model: Reproducing human inflammatory bowel disease etiologies in mice.

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Sun-Min Seo,Na-Won Kim,Eun-Seon Yoo,Ji-Hun Lee,Ah-Reum Kang,Han-Bi Jeong,Won-Yong Shim,Dong-Hyun Kim,Young-Jun Park,Kieun Bae,Kyong-Ah Yoon,Yang-Kyu Choi

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Acacetin protects against acute lung injury by upregulating SIRT1/ NF-κB pathway.

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Journal of cardiothoracic surgery 19:509 PubMed39223627

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Mechanism of KLF2 in young mice with pneumonia induced by Streptococcus pneumoniae.

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Xiaoshuang Li,Weihua Xu,Tao Jing

Fundamental & clinical pharmacology 38:1143-1154 PubMed39128482

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Alamandin and especially melatonin attenuate pulmonary arterial hypertension induced by monocrotalin.

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Seyhan Ayik,Mehmet Gunata,Onural Ozhan,Azibe Yildiz,Nigar Vardi,Emre Sonmez,Necip Ermis,Nilay Ates,Ertugrul Kilic,Samir Abbas Ali Noma,Ahmet Ulu,Seyfullah Taha Inan,Haci Ahmet Acet,Hakan Parlakpinar

Chemical biology & drug design 104:e14579 PubMed39013775

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The protective effect of karanjin against sepsis-induced acute lung injury in mice is involved in the suppression of the TLR4 pathway.

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Chujie Zhang,Juncong Ma,Chang Liu,Xianliang Yan

Redox biology 75:103264 PubMed38972295

2024

Redox-dependent plasticity of oxMIF facilitates its interaction with CD74 and therapeutic antibodies.

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Species

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Sara Sajko,Erin Skeens,Alexander Schinagl,Maroua Ferhat,Irina Mirkina,Julia Mayer,Gregor Rossmueller,Michael Thiele,George P Lisi

View all publications

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websiteProtocolBooklet

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Myeloperoxidase (MPO) Activity Assay试剂盒(Colorimetric)

Myeloperoxidase (MPO) Activity Assay Kit (Colorimetric)

Functional Studies - Myeloperoxidase (MPO) Activity Assay Kit (Colorimetric) (AB105136)

Functional Studies - Myeloperoxidase (MPO) Activity Assay Kit (Colorimetric) (AB105136)

Functional Studies - Myeloperoxidase (MPO) Activity Assay Kit (Colorimetric) (AB105136)

Schematic Diagram - Myeloperoxidase (MPO) Activity Assay Kit (Colorimetric) (AB105136)