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AB139446

MMP2抑制剂Screening Assay试剂盒(Colorimetric)

MMP2 Inhibitor Screening Assay Kit (Colorimetric)

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(8 Publications)

MMP2 Inhibitor Screening Assay Kit (Colorimetric) (ab139446) is a complete assay system designed to screen MMP2 inhibitors using a thiopeptide as a chromogenic substrate (Ac-PLG-[2-mercapto-4-methyl-pentanoyl]-LG-OC2H5).

查看别名

CLG4A, MMP2, 72 kDa type IV collagenase, 72 kDa gelatinase, Gelatinase A, Matrix metalloproteinase-2, TBE-1, MMP-2

4 Images
Functional Studies - MMP2 Inhibitor Screening Assay Kit (Colorimetric) (AB139446)
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Supplier Data

Functional Studies - MMP2 Inhibitor Screening Assay Kit (Colorimetric) (AB139446)

Example Graph for Km and Vmax Determination.

Km=105 μM

Vmax=3.9 pmol/sec

Functional Studies - MMP2 Inhibitor Screening Assay Kit (Colorimetric) (AB139446)
  • FuncS

Supplier Data

Functional Studies - MMP2 Inhibitor Screening Assay Kit (Colorimetric) (AB139446)

Inhibition of MMP3 by NNGH.

Control slope = 1.65E-02 OD/min

Inhibitor slope = 9.80E-04 OD/min

Iinhibitor % activity remaining = (9.80E-04/1.65E-02) x 100 = 5.94%

Functional Studies - MMP2 Inhibitor Screening Assay Kit (Colorimetric) (AB139446)
  • FuncS

Supplier Data

Functional Studies - MMP2 Inhibitor Screening Assay Kit (Colorimetric) (AB139446)

Inhibition of MMP3 by NNGH

Functional Studies - MMP2 Inhibitor Screening Assay Kit (Colorimetric) (AB139446)
  • FuncS

Supplier Data

Functional Studies - MMP2 Inhibitor Screening Assay Kit (Colorimetric) (AB139446)

Plot of OD vs time

关键信息

检测方法

Colorimetric

样品类型

Inhibitor compounds

检测类型

Enzyme activity

检测平台

Microplate reader

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搭配使用产品

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

MMP2 Inhibitor Screening Assay Kit (Colorimetric) (ab139446) is a complete assay system designed to screen MMP2 inhibitors using a thiopeptide as a chromogenic substrate (Ac-PLG-[2-mercapto-4-methyl-pentanoyl]-LG-OC2H5). The MMP cleavage site peptide bond is replaced by a thioester bond in the thiopeptide. Hydrolysis of this bond by an MMP produces a sulfhydryl group, which reacts with DTNB [5,5'-dithiobis(2-nitrobenzoic acid), Ellman's reagent] to form 2-nitro-5-thiobenzoic acid, which can be detected by its absorbance at 412 nm (ε=13,600 M-1cm-1 at pH 6.0 and above). The assays are performed in a convenient 96-well microplate format.

This kit is useful to screen inhibitors of MMP2, a potential therapeutic target. The MMP inhibitor NNGH is also included as a prototypic control inhibitor.

Thiol inhibitors should not be used with this kit, as they may interfere with the colorimetric assay.

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规格

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性能和储存信息

运输条件
Dry Ice
推荐的短期储存条件
-80°C
推荐的长期储存条件
Multi
储存信息
Please refer to protocols
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The MMP-2 protein also known as matrix metalloproteinase-2 or gelatinase A is an enzyme involved in the breakdown of extracellular matrix components. It plays a critical role in tissue remodeling and cell migration. Comprised of a molecular weight of approximately 72 kDa this metalloproteinase is secreted as an inactive proenzyme that requires activation. MMP2 is expressed in various tissues including the brain heart and blood vessels where it contributes to normal physiological processes and pathological conditions.
Biological function summary

Matrix metalloproteinase-2 is mainly involved in the degradation of type IV and V collagens gelatin and fibronectin. As part of the metalloproteinase family it works alongside other MMPs to maintain tissue homeostasis and repair. MMP-2 forms part of a complex network that ensures the timely degradation of matrix components balancing synthesis and breakdown. It remains regulated by tissue inhibitors of metalloproteinases (TIMPs) preventing excessive degradation that could lead to tissue damage.

Pathways

MMP-2 plays a significant role within the extracellular matrix (ECM) remodeling and angiogenesis pathways. It interacts with various proteins including integrins and TIMP-2 to modulate cellular behaviors such as migration and invasion. MMP-2 contributes to processes like wound healing and embryonic development through its involvement in ECM degradation and new tissue formation.

Matrix metalloproteinase-2 is linked to cancer progression and cardiovascular diseases. In cancer abnormal MMP-2 activity facilitates tumor invasion and metastasis by breaking down matrix barriers. Increased MMP-2 expression associates with poor prognosis in cancers like breast and prostate. In cardiovascular diseases such as atherosclerosis it contributes to plaque destabilization and vascular remodeling. The imbalance in MMP-2 activity and its regulation by proteins like TIMP-1 are involved in the pathology of these disorders.
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产品实验方案

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靶点信息

Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues in association with MMP14.. PEX, the C-terminal non-catalytic fragment of MMP2, possesses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels.. Isoform 2. Mediates the proteolysis of CHUK/IKKA and initiates a primary innate immune response by inducing mitochondrial-nuclear stress signaling with activation of the pro-inflammatory NF-kappaB, NFAT and IRF transcriptional pathways.
See full target information MMP2
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文献 (8)

Recent publications for all applications. Explore the full list and refine your search

RSC advances 15:10426-10441 PubMed40182507

2025

A tailored 4G -triazine-based dendrimer vehicle for quercetin endowed with MMP-2/9 inhibition and VEGF downregulation for targeting breast cancer progression and liver metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Doaa R Ramadan,Heba A Osman,Somaya Aly Madhy,Mohamed Teleb,A I Darwish,Marwa M Abu-Serie,Nesreen S Haiba,Sherine N Khattab,Hosam H Khalil

Journal of microbiology and biotechnology 35:e2412027 PubMed39947701

2025

Design and Synthesis of Hydroxamate-Based Matrix Metalloproteinase-2 Inhibitors for Anti-Photoaging.

Applications

Unspecified application

Species

Unspecified reactive species

Jin Young Lee,Geunhyuk Jang,Yung Hyup Joo,Joonho Choi,Dong-Woo Lee,Jae Won Yoo

Journal of enzyme inhibition and medicinal chemistry 39:2388209 PubMed39140776

2024

Non-small cell lung cancer sensitisation to platinum chemotherapy via new thiazole-triazole hybrids acting as dual T-type CCB/MMP-9 inhibitors.

Applications

Unspecified application

Species

Unspecified reactive species

Hassan Gamal,Khadiga A Ismail,A-Mohsen M E Omar,Mohamed Teleb,Marwa M Abu-Serie,Sun Huang,Abdalla S Abdelsattar,Gerald W Zamponi,Hesham Fahmy

International journal of molecular sciences 24: PubMed38069037

2023

Synthesis and Characterization of a New Class of Chromene-Azo Sulfonamide Hybrids as Promising Anticancer Candidates with the Exploration of Their EGFR, CAII, and MMP-2 Inhibitors Based on Molecular Docking Assays.

Applications

Unspecified application

Species

Unspecified reactive species

Fawzia F Alblewi,Mosa H Alsehli,Zainab M Hritani,Areej Eskandrani,Wael H Alsaedi,Majed O Alawad,Ahmed A Elhenawy,Hanaa Y Ahmed,Mohamed S A El-Gaby,Tarek H Afifi,Rawda M Okasha

ChemMedChem 16:3701-3719 PubMed34547831

2021

Engineered s-Triazine-Based Dendrimer-Honokiol Conjugates as Targeted MMP-2/9 Inhibitors for Halting Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hosam H Khalil,Heba A Osman,Mohamed Teleb,A I Darwish,Marwa M Abu-Serie,Sherine N Khattab,Nesreen S Haiba

International journal of molecular sciences 22: PubMed34638665

2021

Halting Tumor Progression via Novel Non-Hydroxamate Triazole-Based Mannich Bases MMP-2/9 Inhibitors; Design, Microwave-Assisted Synthesis, and Biological Evaluation.

Applications

Unspecified application

Species

Unspecified reactive species

Fawzia Faleh Albelwi,Mohamed Teleb,Marwa M Abu-Serie,Mohamed Nabil Abd Al Moaty,Mai S Alsubaie,Mohamed A Zakaria,Yeldez El Kilany,Mohamed Reda Aouad,Mohamed Hagar,Nadjet Rezki

Biomacromolecules 19:1285-1293 PubMed29505725

2018

Macromolecule-Network Electrostatics Controlling Delivery of the Biotherapeutic Cell Modulator TIMP-2.

Applications

Unspecified application

Species

Unspecified reactive species

Yuji Yamada,Ananda Chowdhury,Joel P Schneider,William G Stetler-Stevenson

Biochemistry 56:6423-6433 PubMed29140689

2017

Tissue Inhibitor of Metalloprotease-2 (TIMP-2): Bioprocess Development, Physicochemical, Biochemical, and Biological Characterization of Highly Expressed Recombinant Protein.

Applications

Unspecified application

Species

Unspecified reactive species

Anandã Chowdhury,Robert Brinson,Beiyang Wei,William G Stetler-Stevenson
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