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AB139443

MMP1抑制剂Screening Assay试剂盒(Colorimetric)

MMP1 Inhibitor Screening Assay Kit (Colorimetric)

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(4 Publications)

MMP1 Inhibitor Screening Assay Kit (Colorimetric) (ab139443) is a complete assay system designed to screen MMP1 inhibitors using a thiopeptide as a chromogenic substrate (Ac-PLG-[2-mercapto-4-methyl-pentanoyl]-LG-OC2H5).

查看别名

CLG, MMP1, Interstitial collagenase, Fibroblast collagenase, Matrix metalloproteinase-1, MMP-1

4 Images
Functional Studies - MMP1 Inhibitor Screening Assay Kit (Colorimetric) (AB139443)
  • FuncS

Supplier Data

Functional Studies - MMP1 Inhibitor Screening Assay Kit (Colorimetric) (AB139443)

Inhibition of MMP1 by NNGH (1 µM).

control slope = 1.20E-02 OD/min

inhibitor slope = 2.13E-03 OD/min

inhibitor % activity remaining = (2.13E-03/1.20E-02) x 100 = 17.7%

Functional Studies - MMP1 Inhibitor Screening Assay Kit (Colorimetric) (AB139443)
  • FuncS

Supplier Data

Functional Studies - MMP1 Inhibitor Screening Assay Kit (Colorimetric) (AB139443)

Inhibition of MMP1 by NNGH (1 µM)

Functional Studies - MMP1 Inhibitor Screening Assay Kit (Colorimetric) (AB139443)
  • FuncS

Supplier Data

Functional Studies - MMP1 Inhibitor Screening Assay Kit (Colorimetric) (AB139443)

Plot of OD vs time.

Slope = V = 1.20E-02 OD/min

Functional Studies - MMP1 Inhibitor Screening Assay Kit (Colorimetric) (AB139443)
  • FuncS

Supplier Data

Functional Studies - MMP1 Inhibitor Screening Assay Kit (Colorimetric) (AB139443)

Example graph for Km and Vmax determination.

Km = 1410 μM

Vmax = 40.0 pmol/sec

关键信息

检测方法

Colorimetric

样品类型

Inhibitor compounds

检测类型

Enzyme activity

检测平台

Microplate reader

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搭配使用产品

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MMP2 Inhibitor Screening Assay Kit (Colorimetric)

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反应性数据

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产品详情

MMP1 Inhibitor Screening Assay Kit (Colorimetric) (ab139443) is a complete assay system designed to screen MMP1 inhibitors using a thiopeptide as a chromogenic substrate (Ac-PLG-[2-mercapto-4-methyl-pentanoyl]-LG-OC2H5). The MMP cleavage site peptide bond is replaced by a thioester bond in the thiopeptide. Hydrolysis of this bond by an MMP produces a sulfhydryl group, which reacts with DTNB [5,5'-dithiobis(2-nitrobenzoic acid), Ellman's reagent] to form 2-nitro-5-thiobenzoic acid, which can be detected by its absorbance at 412 nm (e=13,600 M-1cm-1 at pH 6.0 and above). The assays are performed in a convenient 96-well microplate format.

This kit is useful to screen inhibitors of MMP1, a potential therapeutic target. The MMP inhibitor NNGH is also included as a prototypic control inhibitor.

Thiol inhibitors should not be used with this kit, as they may interfere with the colorimetric assay.

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规格

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性能和储存信息

运输条件
Dry Ice
推荐的短期储存条件
-80°C
推荐的长期储存条件
Multi
储存信息
Please refer to protocols
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

MMP1 also known as collagenase-1 is an enzyme belonging to the matrix metalloproteinase (MMP) family. It is responsible for the degradation of type I II and III collagens making it an important player in collagen turnover. MMP1 has a molecular weight of approximately 54 kDa. This enzyme is expressed in various tissues including skin lung and reproductive organs primarily during tissue remodeling and repair. Scientists often use MMP1 ELISA kits and MMP1 inhibitors to study its expression and activity levels.
Biological function summary

MMP1 plays a significant role in the modulation of the extracellular matrix. It facilitates cellular processes by breaking down structural proteins and allowing for cell migration proliferation and differentiation. Although it acts independently MMP1 works in harmony with other MMP family members to maintain extracellular matrix homeostasis and is often linked to tissue remodeling complexes. This makes the MMP1 a valuable target for researchers interested in tissue repair and fibrosis.

Pathways

Studies show MMP1 involvement in critical processes such as the inflammatory response and wound healing. In the inflammatory pathway MMP1 expression is regulated by cytokines that respond to tissue injury. Within the wound healing pathway this enzyme interacts with proteins like transforming growth factor-beta (TGF-beta) to coordinate the repair process by remodeling extracellular matrix components. This illustrates the interconnected nature of MMP1 with significant regulatory proteins.

MMP1 has significant associations with osteoarthritis and cancer metastasis. MMP1 contributes to the breakdown of cartilage in osteoarthritis highlighting its role in disease progression and joint degradation. In the context of cancer elevated MMP1 expression correlates with the metastatic potential where it assists tumor invasion by degrading the surrounding matrix. MMP1's involvement with other MMPs including MMP2 and MMP9 highlights its importance in these pathological conditions making it a therapeutic target in related research.
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产品实验方案

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靶点信息

Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X (PubMed : 1645757, PubMed : 2153297, PubMed : 2557822). In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity (PubMed : 16807369).
See full target information MMP1
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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of microbiology and biotechnology 35:e2412027 PubMed39947701

2025

Design and Synthesis of Hydroxamate-Based Matrix Metalloproteinase-2 Inhibitors for Anti-Photoaging.

Applications

Unspecified application

Species

Unspecified reactive species

Jin Young Lee,Geunhyuk Jang,Yung Hyup Joo,Joonho Choi,Dong-Woo Lee,Jae Won Yoo

International journal of molecular sciences 25: PubMed39596290

2024

Exploring Cannabidiol (CBD) and Cannabigerol (CBG) Safety Profile and Skincare Potential.

Applications

Unspecified application

Species

Unspecified reactive species

Mariana Luz-Veiga,Adélia Mendes,Diana Tavares-Valente,Manuela Amorim,António Conde,Manuela Estevez Pintado,Helena R Moreira,João Azevedo-Silva,João Fernandes

Scientific reports 10:14710 PubMed32895466

2020

Prediction of an MMP-1 inhibitor activity cliff using the SAR matrix approach and its experimental validation.

Applications

Unspecified application

Species

Unspecified reactive species

Yasunobu Asawa,Atsushi Yoshimori,Jürgen Bajorath,Hiroyuki Nakamura

Saudi pharmaceutical journal : SPJ : the official 27:446-452 PubMed30976190

2019

A new perspective on evaluation of medicinal plant biological activities: The correlation between phytomics and matrix metalloproteinases activities of some medicinal plants.

Applications

Unspecified application

Species

Unspecified reactive species

Ekrem-Murat Gonulalan,Emirhan Nemutlu,Lutfiye-Omur Demirezer
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