MMP Activity Assay试剂盒(Fluorometric - Red)

产品详情

MMP Activity Assay Kit (Fluorometric Red) ab112147 uses a fluorescence resonance energy transfer (FRET) peptide as a MMP substrate. In the intact FRET peptide, the fluorescence of one part is quenched by the other. Upon cleavage into two separate fragments by MMPs, the fluorescence is recovered.

The MMP assy is designed to check the general activity of a MMP enzyme in a tissue sample. It can also be used to screen MMP inhibitors when a purified MMP enzyme is used.

With excellent fluorescence quantum yield and longer wavelength, the substrate used in this assay shows less interference from autofluorescence of test compounds and cellular components and is much more sensitive than an EDANS/Dabcyl FRET substrate.

The MMP assay signal can be easily read by a fluorescence microplate reader at Ex/Em = 540/590 nm. The pH-independent fluorescence makes the assay reading available for the whole physiological pH range.

The high photostability of this FRET peptide provides a useful imaging probe. Many labs have used this kit for the high throughput screening of MMP inhibitors as potential anticancer drug candidates. This assay might be also used for monitoring cancer cells.

ab112147 should be stored Desiccated

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.