MMP-3 Activity Assay试剂盒(Fluorometric) (ab118972)
Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Cell culture media, Cell culture supernatant, Milk, Tissue Extracts, Urine
- Sensitivity: 0.05 mU/well
概述
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产品名称
MMP-3 Activity Assay试剂盒(Fluorometric)
参阅全部 MMP3 试剂盒 -
检测方法
Fluorescent -
样品类型
Cell culture supernatant, Milk, Urine, Tissue Extracts, Cell culture media -
检测类型
Enzyme activity -
灵敏度
< 0.05 mU/well -
检测时间
1h 00m -
种属反应性
与反应: Mammals, Other species -
产品概述
In Abcam's MMP-3 Activity Assay Kit, MMP-3 hydrolyzes a specific FRET substrate to release the quenched fluorescent group Mca, which can be detected fluorometrically at Ex/Em=325/393 nm. The kit provides a rapid, simple, sensitive and reliable test which can also be used as a high throughput assay of MMP-3 activity. The assay sensitivity is < 50 µU.
Visit our FAQs page for tips and troubleshooting.
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说明
This product is manufactured by BioVision, an Abcam company and was previously called K783 MMP-3 Activity Fluorometric Assay Kit. K783-100 is the same size as the 100 test size of ab118972.
The matrix metalloproteinase-3 (MMP-3, stromelysin-1) exhibits a number of activities that would make it a particularly good tumor promoter. Like several other MMPs, MMP-3 was first cloned and later recloned as a cancer-specific gene. In addition to degrading numerous extracellular matrix components, MMP-3 can activate gelatinase B, the collagenases and several serpin-type serine proteinase inhibitors. Moreover, it can release a number of cell surface molecules, including E-cadherin, a known contributor to cancer development.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the SDS download section.
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平台
Microplate reader
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 100 tests MCA Peptide Standard 1 x 20µl MMP-1/3 Assay Buffer 1 x 25ml MMP-3 Positive Control 1 vial MMP-3 Substrate 1 x 200µl -
研究领域
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功能
Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase. -
序列相似性
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
结构域
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
细胞定位
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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别名
- CHDS6
- Matrix metalloproteinase 3
- Matrix metalloproteinase 3 preproprotein
see all
图片
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Functional Studies - MMP-3 Activity Assay Kit (ab118972)MMP-3 Positive Control
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Functional Studies - MMP-3 Activity Assay Kit (ab118972)MMP-3 Mca Standard
数据表及文件
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SDS download
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Datasheet download
文献 (14)
ab118972 被引用在 14 文献中.
- O'Callaghan J et al. Matrix metalloproteinase-3 (MMP-3)-mediated gene therapy for glaucoma. Sci Adv 9:eadf6537 (2023). PubMed: 37075118
- Kang MJ et al. Diplacone Isolated from Paulownia tomentosa Mature Fruit Induces Ferroptosis-Mediated Cell Death through Mitochondrial Ca2+ Influx and Mitochondrial Permeability Transition. Int J Mol Sci 24:N/A (2023). PubMed: 37108220
- Kim SJ et al. Avenanthramide-C Shows Potential to Alleviate Gingival Inflammation and Alveolar Bone Loss in Experimental Periodontitis. Mol Cells 46:627-636 (2023). PubMed: 37641936
- Cheng P et al. Smad3 regulates smooth muscle cell fate and mediates adverse remodeling and calcification of the atherosclerotic plaque. Nat Cardiovasc Res 1:322-333 (2022). PubMed: 36246779
- Rossi M et al. PHGDH heterogeneity potentiates cancer cell dissemination and metastasis. Nature 605:747-753 (2022). PubMed: 35585241