JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB109906

MitoTox™ Complex IV OXPHOS Activity Assay试剂盒

MitoTox™ Complex IV OXPHOS Activity Assay Kit

Be the first to review this product! Submit a review

|

(9 Publications)

MitoTox™ Complex IV OXPHOS Activity Assay Kit (ab109906) is designed for testing the direct inhibitory effect of compounds on Complex IV activity in only 5 hours.

查看别名

COX4, COX4I1, Cytochrome c oxidase polypeptide IV, Cytochrome c oxidase subunit IV isoform 1, COX IV-1

1 Images
Functional Studies - MitoTox™ Complex IV OXPHOS Activity Assay Kit (AB109906)
  • FuncS

Supplier Data

Functional Studies - MitoTox™ Complex IV OXPHOS Activity Assay Kit (AB109906)

Typical dose response curve for KCN. Assay was performed following the Dose Response Assay Procedure using KCN, a well known Complex IV inhibitor. KCN was prepared in 0.1 M NaOH to generate a 100 mM stock. Starting with a 100 μM final concentration in well, 1 : 10 serial dilutions of KCN were generated.

关键信息

检测方法

Colorimetric

反应种属

Cow, Human

检测类型

Direct

检测时间

5h

检测平台

Microplate reader

style
left-column

搭配使用产品

Assay Kits

AB109721

Complex I Enzyme Activity Microplate Assay Kit (Colorimetric)

Functional Studies - Complex I Enzyme Activity Microplate Assay Kit (Colorimetric) (AB109721)

  • 5
  • 5
Assay Kits

AB228560

Succinate Dehydrogenase Activity Assay Kit (Colorimetric)

Functional Studies - Succinate Dehydrogenase Activity Assay Kit (Colorimetric) (AB228560)

  • 0
  • 0
Assay Kits

AB287844

Mitochondrial Complex III Activity Assay Kit

Functional Studies - Mitochondrial Complex III Activity Assay Kit (AB287844)

  • 0
  • 0
Assay Kits

AB109714

ATP synthase Activity Assay Kit (Colorimetric)

Functional Studies - ATP synthase Activity Assay Kit (Colorimetric) (AB109714)

  • 5
  • 1
style
left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

产品详情

MitoTox™ Complex IV OXPHOS Activity Assay Kit (ab109906) is designed for testing the direct inhibitory effect of compounds on Complex IV activity in only 5 hours. Complex IV extracted from the provided bovine heart mitochondria (a rich source of Complex IV) is immunocaptured by specific antibodies on the plate. Complex IV activity can be observed as decrease in absorbance at OD 550 nm. The intra-assay and inter-assay variation of this assay are both <10%.

Inhibitory effects of compounds on Complex IV activity can be tested in two different ways: 1. Screening format, where up to 23 compounds can be tested at a single concentration in triplicate; 2. Dose response (IC50) format, where two compounds known to affect Complex IV activity can be tested at 11 different data points in triplicate.

Testing for mitochondrial function has become a key aspect of drug discovery. Mitochondria can be affected by drug treatment, resulting into cardio- and hepatotoxic side effects that can lead to drug withdrawal from the market. Therefore, there is increasing emphasis on testing the impact on mitochondria early on in the drug development process to reduce failure rates during preclinical and clinical phases.

Store Bovine Heart Mitochondria and Reagent C at -80°C. Store all other components at 4°C.

Related products

Review the mitochondrial assay guide, or the full metabolism assay guide to learn about more assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also how to assay metabolic function in live cells using your plate reader.

style
left-column

规格

{ "values": { "96Test": { "sellingSize": "96 Test", "publicAssetCode":"ab109906-96Test", "assetComponentDetails": [ { "size":"1 x 1.8 g", "name":"Blocking Powder", "number":"AB109906-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pre-coated 96-well microplate", "number":"AB109906-CMP06", "productcode":"" }, { "size":"1 x 20 µL", "name":"Detergent", "number":"AB109906-CMP01", "productcode":"" }, { "size":"1 x 70 mL", "name":"1X Blocking Buffer", "number":"AB109906-CMP03", "productcode":"" }, { "size":"2 x 1 Unit", "name":"12-channel reagent reservoirs", "number":"AB109906-CMP02", "productcode":"" }, { "size":"1 x 110 mL", "name":"1X Complex IV Mito Buffer", "number":"AB109906-CMP04", "productcode":"" }, { "size":"1 x 90 µL", "name":"Bovine Heart Mitochondria", "number":"AB109906-CMP08", "productcode":"" }, { "size":"2 x 1 mL", "name":"Reagent C (Reduced Cytochrome c)", "number":"AB109906-CMP07", "productcode":"" } ] } } }
style
left-column

性能和储存信息

运输条件
Dry Ice
推荐的短期储存条件
Multi
推荐的长期储存条件
Multi
储存信息
Please refer to protocols
style
left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Complex IV also known as cytochrome c oxidase is an important component of the electron transport chain in mitochondria. This enzyme complex has significant mass approximately 204 kDa and operates primarily in the inner mitochondrial membrane. It catalyzes the transfer of electrons from cytochrome c to oxygen facilitating ATP generation. Complex IV is ubiquitously expressed in all tissues with higher expression in organs with high energy demands like heart and skeletal muscle. This enzyme is composed of multiple subunits encoded by both mitochondrial and nuclear DNA.
Biological function summary

Cytochrome c oxidase plays a critical role in cellular respiration. It forms part of the larger enzyme complex which also includes Complex I II and III. These complexes work together to create a proton gradient across the inner mitochondrial membrane essential for ATP synthesis via oxidative phosphorylation. This enzyme's activity is measured through assays like the cytochrome c oxidase assay which evaluates its function in various tissues. These assays help researchers understand how effectively electrons are being transferred and protons are driven across the membrane.

Pathways

Complex IV is integral to the oxidative phosphorylation pathway and the broader mitochondrial respiratory chain. It interacts closely with cytochrome c a small heme protein that shuttles electrons between Complex III (cytochrome c reductase) and Complex IV. Through these interactions the proton gradient is established enabling ATP synthase to convert chemical energy into usable cell energy. This process significantly impacts cellular metabolism and energy production influencing how efficiently cells function.

Complex IV dysfunction is associated with mitochondrial diseases and certain neurodegenerative disorders. For instance defects in cytochrome c oxidase lead to conditions like Leigh syndrome a severe neurological disorder. Also studies show that disruptions in the electron transport chain involving Complex IV relate closely to Alzheimer’s disease. Alterations in proteins like cytochrome c which work in tandem with Complex IV can exacerbate these conditions highlighting the importance of this target in understanding and potentially treating these diseases.
style
left-column

产品实验方案

style
left-column

靶点信息

Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.
See full target information COX4I1
style
left-column

文献 (9)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 11:15 PubMed39828731

2025

CRAT downregulation promotes ovarian cancer progression by facilitating mitochondrial metabolism through decreasing the acetylation of PGC-1α.

Applications

Unspecified application

Species

Unspecified reactive species

Zhen Zhang,Shuhua Zhao,Xiaohui Lv,Yan Gao,Qian Guo,Yanjie Ren,Yuanyuan He,Yihua Jin,Hong Yang,Shujuan Liu,Xiaohong Zhang

Clinical and experimental pharmacology & physiology 51:e13860 PubMed38584327

2024

NPAS2, transcriptionally activated by ARRB1, promotes the malignant behaviours of lung adenocarcinoma cells and regulates the reprogramming of glucose metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Shenglan Wang,Chunhong Huang,Yanbin Zheng,Xinjie Wu,Yutong Zhong

Frontiers in endocrinology 13:895593 PubMed35957832

2022

plays an important role in the pathogenesis of metabolic-associated fatty liver disease by regulating hepatic lipid metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Yongqiang Ma,Guangshun Chen,Junfang Yi,Qiang Li,Zhi Tan,Wenling Fan,Xiaohua Luo,Zhiyong He,Zhongzhou Si,Jiequn Li

Communications biology 4:775 PubMed34163008

2021

Cyanocobalamin prevents cardiomyopathy in type 1 diabetes by modulating oxidative stress and DNMT-SOCS1/3-IGF-1 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Masao Kakoki,Purushotham V Ramanathan,John R Hagaman,Ruriko Grant,Jennifer C Wilder,Joan M Taylor,J Charles Jennette,Oliver Smithies,Nobuyo Maeda-Smithies

Cancer discovery 11:2316-2333 PubMed33893149

2021

NKX3.1 Localization to Mitochondria Suppresses Prostate Cancer Initiation.

Applications

Unspecified application

Species

Unspecified reactive species

Alexandros Papachristodoulou,Antonio Rodriguez-Calero,Sukanya Panja,Elizabeth Margolskee,Renu K Virk,Teresa A Milner,Luis Pina Martina,Jaime Y Kim,Matteo Di Bernardo,Alanna B Williams,Elvis A Maliza,Joseph M Caputo,Christopher Haas,Vinson Wang,Guarionex Joel De Castro,Sven Wenske,Hanina Hibshoosh,James M McKiernan,Michael M Shen,Mark A Rubin,Antonina Mitrofanova,Aditya Dutta,Cory Abate-Shen

Cell reports 29:1511-1523.e5 PubMed31693892

2019

Metformin Improves Mitochondrial Respiratory Activity through Activation of AMPK.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Wang,Hongying An,Ting Liu,Caolitao Qin,Hiromi Sesaki,Shaodong Guo,Sally Radovick,Mehboob Hussain,Akhil Maheshwari,Fredric E Wondisford,Brian O'Rourke,Ling He

Cancer cell 36:84-99.e8 PubMed31287994

2019

Mubritinib Targets the Electron Transport Chain Complex I and Reveals the Landscape of OXPHOS Dependency in Acute Myeloid Leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Irène Baccelli,Yves Gareau,Bernhard Lehnertz,Stéphane Gingras,Jean-François Spinella,Sophie Corneau,Nadine Mayotte,Simon Girard,Mélanie Frechette,Valérie Blouin-Chagnon,Koryne Leveillé,Isabel Boivin,Tara MacRae,Jana Krosl,Clarisse Thiollier,Vincent-Philippe Lavallée,Evgeny Kanshin,Thierry Bertomeu,Jasmin Coulombe-Huntington,Corinne St-Denis,Marie-Eve Bordeleau,Geneviève Boucher,Philippe P Roux,Sébastien Lemieux,Mike Tyers,Pierre Thibault,Josée Hébert,Anne Marinier,Guy Sauvageau

Nature 510:397-401 PubMed24828042

2014

The metabolite α-ketoglutarate extends lifespan by inhibiting ATP synthase and TOR.

Applications

Unspecified application

Species

Unspecified reactive species

Randall M Chin,Xudong Fu,Melody Y Pai,Laurent Vergnes,Heejun Hwang,Gang Deng,Simon Diep,Brett Lomenick,Vijaykumar S Meli,Gabriela C Monsalve,Eileen Hu,Stephen A Whelan,Jennifer X Wang,Gwanghyun Jung,Gregory M Solis,Farbod Fazlollahi,Chitrada Kaweeteerawat,Austin Quach,Mahta Nili,Abby S Krall,Hilary A Godwin,Helena R Chang,Kym F Faull,Feng Guo,Meisheng Jiang,Sunia A Trauger,Alan Saghatelian,Daniel Braas,Heather R Christofk,Catherine F Clarke,Michael A Teitell,Michael Petrascheck,Karen Reue,Michael E Jung,Alison R Frand,Jing Huang

ACS chemical biology 8:257-67 PubMed23138533

2012

Integrated compound profiling screens identify the mitochondrial electron transport chain as the molecular target of the natural products manassantin, sesquicillin, and arctigenin.

Applications

Unspecified application

Species

Unspecified reactive species

Kevin Lai,Douglas W Selinger,Jonathan M Solomon,Hua Wu,Esther Schmitt,Fabrizio C Serluca,Daniel Curtis,John D Benson
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com