Mitochondrial Staining试剂- Green - Cytopainter (ab176830)
概述
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产品名称
Mitochondrial Staining试剂- Green - Cytopainter
参阅全部 Mitochondria 试剂盒 -
样品类型
Adherent cells, Suspension cells -
检测类型
Cell-based (qualitative) -
种属反应性
与反应: Mammals, Other species -
产品概述
Abcam's Mitochondrial Staining Reagent - Green | Cytopainter (ab176830) is part of a series of new fluorogenic probes to label mitochondria of live cells. The proprietary mitochondria dye selectively accumulates in mitochondria probably via the mitochondrial membrane potential gradient. The mitochondrial indicator is a hydrophobic compound that easily permeates intact live cells and becomes trapped in mitochondria after it gets into cells. The fluorescent mitochondrial indicator is retained in mitochondria for a long time since it carries a cell-retaining group. This key feature significantly increases the staining efficiency; make it useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity.
Please note the dye will stain live cells but is not well-retained after fixation.
Review other dyes and kits for mitochondrial staining, or the live cell staining fluorescent dyes guide
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说明
Mitochondria are membrane-enclosed organelles found in most eukaryotic cells. Mitochondria are sometimes described as "cellular power plants" because they generate most of the cellular supply of ATP. In addition to supplying cellular energy, mitochondria are involved in a range of other processes, such as signaling, cellular differentiation, cell death, as well as the control of the cell cycle and cell growth. Mitochondria have been implicated in several human diseases, including mitochondrial disorders and cardiac dysfunction, and may play a role in the aging process. Although most of a cellular DNA is contained in the cell nucleus, the mitochondrion has its own independent genome.
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经测试应用
适用于: FM, Flow Cytmore details -
平台
Fluorescence microscope
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 500 tests CytoPainter MitoGreen Indicator Reagent 1 x 500 tests -
研究领域
相关产品
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Corresponding kit
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab176830于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| FM |
Use at an assay dependent concentration.
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| Flow Cyt |
Use at an assay dependent concentration.
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| 说明 |
|---|
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FM
Use at an assay dependent concentration. |
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Flow Cyt
Use at an assay dependent concentration. |
图片
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U2OS cells stained with CytoPainter MitoGreen Indicator Reagent (ab176830)
Image of U2OS cells stained with Abcam's CytoPainter MitoGreen Indicator Reagent (ab176830) in a Costar black wall/clear bottom 96-well plate.
数据表及文件
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Datasheet download
文献 (4)
ab176830 被引用在 4 文献中.
- Kitisin T et al. The study of tryptophol containing emulgel on fungal reduction and skin irritation. Sci Rep 13:18881 (2023). PubMed: 37919393
- Zhao W et al. Construction of Messenger RNA (mRNA) Probes Delivered By Lipid Nanoparticles to Visualize Intracellular Protein Expression and Localization at Organelles. Adv Mater 33:e2103131 (2021). PubMed: 34541724
- Hassing B et al. Phosphatidic acid produced by phospholipase D is required for hyphal cell-cell fusion and fungal-plant symbiosis. Mol Microbiol 113:1101-1121 (2020). PubMed: 32022309
- Huang CC et al. Autophagy-Regulated ROS from Xanthine Oxidase Acts as an Early Effector for Triggering Late Mitochondria-Dependent Apoptosis in Cathepsin S-Targeted Tumor Cells. PLoS One 10:e0128045 (2015). PubMed: 26029922
