为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    按产品类型
    蛋白质与肽
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 定制化产品 & 合作关系
    定制化产品 & 合作关系

    定制化产品和商务合作加速您的诊断和治疗项目

    定制化产品

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

Kits/ Lysates/ Other Kits Molecular Biology Kits Cell Fractionation Kits

Mammalian Cell Lysis缓冲液5X (ab179835)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Q&A (3)References (29)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

运输费用

Promotion Information

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Key features and details

  • Sample type: Adherent cells, Suspension cells, Tissue

你可能会感兴趣

ELISA
Product image
HSP70 High Sensitivity ELISA Kit (ab133061)
试剂盒
Product image
Deproteinizing Sample Preparation Kit - TCA (ab204708)
准备
Product image
10kD Spin Column (ab93349)

查看更多相关产品

概述

  • 产品名称

    Mammalian Cell Lysis缓冲液5X
  • 样品类型

    Tissue, Adherent cells, Suspension cells
  • 产品概述

    Mammalian Cell Lysis Buffer 5X (ab179835) is widely used to prepare mammalian cell and tissue lysates for use in a variety of downstream biochemical assays, especially those for quantification of enzymatic activity. This buffer does not contain SDS, a reagent that interferes with many activity assays.


    The lysis buffer has been optimized for mammalian samples, but it can also be used for preparation of plant or bacterial samples.


    The buffer just requires a simple 5-fold dilution, and minimal hands-on-time. Cell lysates are ready in only 15 minutes.

  • 说明

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

性能

  • 存放说明

    Store at +4°C. Please refer to protocols.
  • 组件 10 ml
    Mammalian Cell lysis buffer 5X 1 x 10ml
  • 研究领域

    • Kits/ Lysates/ Other
    • Kits
    • Molecular Biology Kits
    • Cell Fractionation Kits
    • Kits/ Lysates/ Other
    • Tools and Reagents
    • Lysis

实验方案

  • Protocol Booklet

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (29)

发表研究结果有使用 ab179835?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab179835 被引用在 29 文献中.

  • Li YG  et al. Cinnamaldehyde protects cardiomyocytes from oxygen-glucose deprivation/reoxygenation-induced lipid peroxidation and DNA damage via activating the Nrf2 pathway. Chem Biol Drug Des 103:e14489 (2024). PubMed: 38404216
  • Zanuttigh E  et al. Identification of Autophagy as a Functional Target Suitable for the Pharmacological Treatment of Mitochondrial Membrane Protein-Associated Neurodegeneration (MPAN) In Vitro. Pharmaceutics 15:N/A (2023). PubMed: 36678896
  • Haidar M  et al. miR-34c-3p Regulates Protein Kinase A Activity Independent of cAMP by Dicing prkar2b Transcripts in Theileria annulata-Infected Leukocytes. mSphere 8:e0052622 (2023). PubMed: 36847534
  • Simchi L  et al. Elevated ROS levels during the early development of Angelman syndrome alter the apoptotic capacity of the developing neural precursor cells. Mol Psychiatry 28:2382-2397 (2023). PubMed: 36991133
  • Bedia C  et al. A Multi-Level Systems Biology Analysis of Aldrin's Metabolic Effects on Prostate Cancer Cells. Proteomes 11:N/A (2023). PubMed: 37092452
View all Publications for this product

客户评价及客户问答

Show All 评价 Q&A
提交评价 提交问题

1-3 of 3 Abreviews or Q&A

Question

what is the pH of this buffer?

Read More

Abcam community

Verified customer

Asked on Jul 21 2015

Answer

Thank you for contacting us.



The buffer is pH 7.

Read More

Heather Allen

Abcam Scientific Support

回复于 Jul 21 2015

Question

Did the mammalian cell lysis buffer contain SDS? i need to know because i want to dose the protein extracted by Bradfoerd. Can i use it for tissu lysis like muscles? and how much 1x buffer i have to use for 20mg of tissu?

Read More

Abcam community

Verified customer

Asked on Nov 07 2014

Answer


I can confirm that this lysis buffer does not contain SDS.

This lysis buffer can be used to lyse tissues and typically, we would recommend ˜400 ul for 20 mg of tissue.

Read More

Elisa Thomas

Abcam Scientific Support

回复于 Nov 07 2014

Question

Do you have any suggestion about which would be the most appropriate lysis buffer in order to keep the enzyme in its active form?

Read More

Abcam community

Verified customer

Asked on Jun 13 2014

Answer

Our Mammalian Cell Lysis Buffer 5X (ab179835) would be the best lysis buffer to use.

<1%Chaps also is OK. We have not checked NP40 and RIPA, but they should be OK theoretically.

Read More

Elisa Thomas

Abcam Scientific Support

回复于 Jun 13 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

通过邮件收到学术资源和促销信息 注册
研究类别(A-Z)
  • 肿瘤研究
  • 心血管研究
  • 细胞生物学
  • 发育生物学
  • 染色质及细胞核信号转导研究
  • 免疫学
  • 代谢
  • 微生物学
  • 神经生物学
  • 信号转导研究
  • 干细胞研究
产品种类(A-Z)
  • 一抗
  • 二抗
  • 生化试剂
  • 亚类对照
  • 流式细胞仪多色选择器
  • 试剂盒
  • 内参对照
  • 细胞裂解液
  • 多肽
  • 蛋白酶
  • 组织切片
  • 蛋白标签及细胞标记物
  • 相关试剂及工具
帮助与支持
  • 技术支持
  • 技术FAQ
  • 实验步骤与建议
  • 购买FAQ
  • RabMAb 产品
  • 培训课程
  • 按靶标浏览
企业
  • 企业资讯
  • 投资者关系
  • 公司新闻
  • 招贤纳士
  • 关于我们
活动通知
  • 商品展览
  • 会议
全球网站
  • abcam.com
  • abcam.co.jp
QR code for Weechat

微信扫码 关注我们

沪ICP备14046373号-1

沪公网安备 31011502007413号

  • 销售条款
  • 网站使用条款
  • Cookie政策
  • 隐私权政策
  • 法律
© 1998-2025 艾博抗(上海)贸易有限公司版权所有