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AB83391

Malate Assay试剂盒

Malate Assay Kit

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(6 Publications)

Malate Assay Kit is an easy and sensitive assay to measure the L(-) Malate level in a variety of samples.
2 Images
Functional Studies - Malate Assay Kit (AB83391)
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Functional Studies - Malate Assay Kit (AB83391)

Example of Standard Curve

Schematic Diagram - Malate Assay Kit (AB83391)
  • Schematic Diagram

Supplier Data

Schematic Diagram - Malate Assay Kit (AB83391)

Representative image of Malate Assay Kit ab83391

Components shown from left to right :

- Malate Standar

- Developer Solution III

- Malate Enzyme Mix

- Assay Buffer 64

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

关键信息

检测方法

Colorimetric

样品类型

Food samples, Tissue Extracts

检测类型

Quantitative

灵敏度

= 20 µM

范围

20 - 200 µM

检测时间

1h

检测平台

Microplate reader

产品详情

Abcam's Malate Assay Kit is an easy and sensitive assay to measure the L(-) Malate level in a variety of samples. In the assay, malate is specifically oxidized to generate a product which reacts with a substrate probe to generate color (λmax = 450 nm). The assay can detect 1~10 nmol of Malate in a 50 μl sample with a detection sensitivity ~20 μM.

Visit our FAQs page for tips and troubleshooting.

This product is manufactured by BioVision, an Abcam company and was previously called K637 Malate Colorimetric Assay Kit. K637-100 is the same size as the 100 test size of ab83391.

L(-) Malate is a TCA cycle intermediate. It plays an important role in the Calvin cycle during carbon fixation in plants. In lower organisms, malate is converted to lactate during malolactic fermentation with the formation of CO2. Malate is frequently used as an additive in the food and pharmaceutical industries, so quantitating malic acid is important in manufacturing beer, wine, cheese and fruits, among others.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

规格

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab83391-100Test", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"Malate Enzyme Mix", "number":"AB83391-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Developer Solution III", "number":"AB83391-CMP04", "productcode":"" }, { "size":"1 x 25 mL", "name":"Assay Buffer 64", "number":"AB83391-CMP01", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Malate Standard", "number":"AB83391-CMP03", "productcode":"" } ] } } }

性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
-20°C
储存信息
-20°C

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Malate often referred to as "malate ion" or simply "malate" is a dicarboxylic acid with the molecular formula C4H6O5 and a molar mass of 134.09 g/mol. It plays a mechanical role as an intermediate in several key metabolic processes. Malate is formed in the mitochondria during the citric acid cycle also known as the Krebs cycle. It is also important in malate-aspartate shuttle contributing to the transport of reducing equivalents across the mitochondrial membrane. Various tissues including liver heart and skeletal muscles express malate highlighting its widespread involvement in metabolic functions.
Biological function summary

The activity of malate is linked to energy production within cells. It is involved in the citric acid cycle converting to oxaloacetate via the enzyme malate dehydrogenase whereby NAD+ is reduced to NADH. This conversion contributes to the cycle that generates energy stored in ATP. Malate is also part of the malate-aspartate shuttle a complex that facilitates the transfer of reducing equivalents produced in glycolysis into the mitochondria. These functions enable cells to efficiently produce ATP which is essential for various cellular activities.

Pathways

The role of malate is important in bioenergetic pathways such as the citric acid cycle and gluconeogenesis. In the citric acid cycle malate’s conversion to oxaloacetate by malate dehydrogenase allows continuation of the cycle. Malate serves as a substrate for the malate-aspartate shuttle which transfers electrons across mitochondrial membranes linked to proteins such as aspartate aminotransferase. Through these pathways malate collaborates closely with proteins like NADH and FADH2 which are essential in energy production and metabolic balance within the cell.

Malate's involvement has implications in conditions like cancer and metabolic disorders. In cancer alterations in metabolic pathways can shift to favor malate production supporting cancerous growth. Additionally in some metabolic disorders imbalances in malate production or utilization can occur affecting energy homeostasis. Connections exist for instance between malate and mutated forms of isocitrate dehydrogenase (IDH) in cancers which can lead to disruptions in normal cellular metabolism and contribute to oncogenic processes.

产品实验方案

文献 (6)

Recent publications for all applications. Explore the full list and refine your search

Journal of biochemical and molecular toxicology 38:e23854 PubMed39287333

2024

Malate dehydrogenase-2 inhibition shields renal tubular epithelial cells from anoxia-reoxygenation injury by reducing reactive oxygen species.

Applications

Unspecified application

Species

Unspecified reactive species

Georgios Pissas,Maria Tziastoudi,Maria Divani,Christina Poulianiti,Maria Anna Polyzou Konsta,Evangelos Lykotsetas,Vasilios Liakopoulos,Ioannis Stefanidis,Theodoros Eleftheriadis

Frontiers in oncology 12:912942 PubMed35847841

2022

The Molecular Role of HIF1α Is Elucidated in Chronic Myeloid Leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Vivek Singh,Ranjana Singh,Rashmi Kushwaha,Shailendra Prasad Verma,Anil Kumar Tripathi,Abbas Ali Mahdi

Proceedings of the National Academy of Sciences of the United States of America 118: PubMed34551979

2021

Functional succinate dehydrogenase deficiency is a common adverse feature of clear cell renal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ritesh K Aggarwal,Rebecca A Luchtel,Venkata Machha,Alexander Tischer,Yiyu Zou,Kith Pradhan,Nadia Ashai,Nandini Ramachandra,Joseph M Albanese,Jung-In Yang,Xiaoyang Wang,Srinivas Aluri,Shanisha Gordon,Ahmed Aboumohamed,Benjamin A Gartrell,Sassan Hafizi,James Pullman,Niraj Shenoy

Nature communications 10:2767 PubMed31235694

2019

Skeletal muscle PGC-1α1 reroutes kynurenine metabolism to increase energy efficiency and fatigue-resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Leandro Z Agudelo,Duarte M S Ferreira,Shamim Dadvar,Igor Cervenka,Lars Ketscher,Manizheh Izadi,Liu Zhengye,Regula Furrer,Christoph Handschin,Tomas Venckunas,Marius Brazaitis,Sigitas Kamandulis,Johanna T Lanner,Jorge L Ruas

Molecular cell 69:87-99.e7 PubMed29249655

2017

The PLAG1-GDH1 Axis Promotes Anoikis Resistance and Tumor Metastasis through CamKK2-AMPK Signaling in LKB1-Deficient Lung Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Lingtao Jin,Jaemoo Chun,Chaoyun Pan,Avi Kumar,Guojing Zhang,Youna Ha,Dan Li,Gina N Alesi,Yibin Kang,Lu Zhou,Wen-Mei Yu,Kelly R Magliocca,Fadlo R Khuri,Cheng-Kui Qu,Christian Metallo,Taofeek K Owonikoko,Sumin Kang

Journal of bacteriology 195:220-30 PubMed23123906

2012

Roles of AtpI and two YidC-type proteins from alkaliphilic Bacillus pseudofirmus OF4 in ATP synthase assembly and nonfermentative growth.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Liu,David B Hicks,Terry A Krulwich
View all publications
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