Lysosomal Staining试剂- Blue - Cytopainter (ab176825)
概述
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产品名称
Lysosomal Staining试剂- Blue - Cytopainter
参阅全部 Lysosomes 试剂盒 -
样品类型
Adherent cells, Suspension cells -
检测类型
Cell-based (qualitative) -
种属反应性
与反应: Mammals, Other species -
产品概述
Lysosomal Staining Reagent - Blue | Cytopainter (ab176825) is part of a series of new fluorogenic probes to label lysosomes of live cells. The proprietary lysotropic dye selectively accumulates in lysosomes probably via the lysosome pH gradient. The lysotropic indicator is a hydrophobic compound that easily permeates intact live cells, and becomes trapped in lysosomes after it gets into the cells. Its fluorescence is significantly enhanced upon entering lysosomes. This key feature significantly reduces its staining background and makes it useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. It is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells.
Review other dyes and kits for lysosomal staining, or the live cell staining fluorescent dyes guide
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说明
Lysosomes are cellular organelles which contain acid hydrolase enzymes to break up waste materials and cellular debris. Lysosomes digest excess or worn-out organelles, food particles, and engulfed viruses or bacteria. The membrane around a lysosome allows the digestive enzymes to work at pH 4.5. The interior of the lysosomes is acidic (pH 4.5 – 4.8) compared to the slightly alkaline cytosol (pH 7.2). The lysosome maintains the pH differential by pumping protons from the cytosol across the membrane via proton pumps and chloride ion channels.
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平台
Fluorescence microscope
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 500 tests CytoPainter LysoBlue Indicator Reagent 1 x 500 tests -
研究领域
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别名
- lysosome marker
- lysotracker
相关产品
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Corresponding kit
图片
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Image of U2OS cells stained with CytoPainter LysoBlue Indicator Reagent (ab176825)
Image of U2OS cells stained with Abcam's CytoPainter LysoBlue Indicator Reagent (ab176825) in a Costar black wall/clear bottom 96-well plate.
数据表及文件
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Datasheet download
文献 (0)
ab176825 尚未被引用在任何文献中。
