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AB228546

Luciferase Reporter Assay底物试剂盒- Renilla

Luciferase Reporter Assay Substrate Kit - Renilla

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(2 Publications)

Luciferase Reporter Assay Substrate Kit - Renilla (ab228546) provides a fast and sensitive method to detect the luciferase from sea pansy (Renilla reniformis).
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Functional Studies - Luciferase Reporter Assay Substrate Kit - Renilla (AB228546)
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Supplier Data

Functional Studies - Luciferase Reporter Assay Substrate Kit - Renilla (AB228546)

Luciferase titration curve.

Renilla Luciferase was measured with Luciferase Reporter Assay Substrate Kit – Renilla (ab228546) in a white 96-well plate.

关键信息

检测方法

Luminescent

样品类型

Cell culture extracts, Adherent cells

检测平台

Microplate reader

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

产品详情

Luciferase Reporter Assay Substrate Kit - Renilla (ab228546) provides a fast and sensitive method to detect the luciferase from sea pansy (Renilla reniformis). It uses a proprietary luminogenic formulation to quantify Renilla luciferase activity in cell-based assays. The formulation generates a luminescent product that gives strong luminescence upon interaction with Renilla luciferase. The kit provides all the essential components. It has high sensitivity and can be performed in a convenient 96-well and 384-well microtiter-plate format. The "glow-type" signal with a half-life of one hour provides a consistent signal across large number of assay plates. The assay is compatible with standard cell growth media. This kit enables the measurement of primary expression or gene expression with wild type and the synthetic hRluc genes.

Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase. The most versatile reporter gene is the firefly luciferase. Recently there is steadily increasing use of other luciferases, such as Renilla luciferase since these reporters are smaller and do not require the presence of ATP.

规格

{ "values": { "1Unit": { "sellingSize": "1 Unit", "publicAssetCode":"ab228546-1Unit", "assetComponentDetails": [ { "size":"1 x 10 mL", "name":"Assay Buffer", "number":"AB228546-CMP01", "productcode":"" }, { "size":"1 x 100 µL", "name":"100X Luciferase Substrate", "number":"AB228546-CMP02", "productcode":"" } ] } } }

性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
-20°C
储存信息
-20°C

产品实验方案

靶点信息

文献 (2)

Recent publications for all applications. Explore the full list and refine your search

BMC pulmonary medicine 22:443 PubMed36434577

2022

CircRNA has_circ_0017109 promotes lung tumor progression via activation of Wnt/β-catenin signaling due to modulating miR-671-5p/FZD4 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Yang,Bin Zhang,Qi Qi,Changli Wang

PLoS neglected tropical diseases 14:e0008730 PubMed33206639

2020

Antigenicity, stability, and reproducibility of Zika reporter virus particles for long-term applications.

Applications

Unspecified application

Species

Unspecified reactive species

J Charles Whitbeck,Anu Thomas,Kathryn Kadash-Edmondson,Ariadna Grinyo-Escuer,Lewis J Stafford,Celine Cheng,Grant C Liao,Frederick W Holtsberg,M Javad Aman,Graham Simmons,Edgar Davidson,Benjamin J Doranz
View all publications
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