AB204721

Lipoprotein Lipase Assay试剂盒(Fluorometric)

Name: Lipoprotein Lipase检测试剂盒 (Fluorometric) (ab204721)| Abcam中文官网
Brand: Abcam Limited
SKU: AB204721
Price: 8477 CNY
Availability: InStock

Lipoprotein Lipase Assay Kit (Fluorometric)

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(15 Publications)

Lipoprotein Lipase Assay Kit (Fluorometric) ab204721 is a simple and accurate assay for measuring Lipoprotein Lipase (LPL) activity.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

查看别名

LIPD, LPL, Lipoprotein lipase, Phospholipase A1

5 Images

Functional Studies - Lipoprotein Lipase Assay Kit (Fluorometric) (AB204721)

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Supplier Data

Functional Studies - Lipoprotein Lipase Assay Kit (Fluorometric) (AB204721)

Measurement of LPL activity in purified enzyme from Pseudomonas sp. (5 ng), post-heparin treated mouse plasma (2 μl), 7-day post-differentiated 3T3-L1 cell lysate (100 μg), and rat heart lysate (200 μg)

FuncS

Supplier Data

Functional Studies - Lipoprotein Lipase Assay Kit (Fluorometric) (AB204721)

Inhibition of Positive Control by Orlistat, a generic lipase inhibitor. The assay was run for 1 hour and the IC₅₀ was determined to be 11.4 μM.

FuncS

Supplier Data

Functional Studies - Lipoprotein Lipase Assay Kit (Fluorometric) (AB204721)

Inhibition of LPL activity from post-heparin treated mouse plasma by Angptl 4, an LPL specific inhibitor. The assay was run for 1 hour and the activity was determined by calculating the slope. The IC₅₀ was determined to be 22.6 nM.

FuncS

Supplier Data

Functional Studies - Lipoprotein Lipase Assay Kit (Fluorometric) (AB204721)

Typical LPL Substrate Standard Curve.

Schematic Diagram - Lipoprotein Lipase Assay Kit (Fluorometric) (AB204721)

Schematic Diagram

Supplier Data

Schematic Diagram - Lipoprotein Lipase Assay Kit (Fluorometric) (AB204721)

Representative image of Lipoprotein Lipase Assay Kit (Fluorometric) ab204721

Components shown from left to right :

- LPL Substrate

- LPL Inhibitor

- LPL Positive Control

- LPL Assay Buffer

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

关键信息

检测方法

Fluorescent

样品类型

Purified protein, Tissue Lysate, Plasma, Cell Lysate

检测类型

Enzyme activity

检测时间

20m

检测平台

Microplate reader

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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产品详情

Lipoprotein Lipase Assay Kit (Fluorometric) ab204721 is a simple and accurate assay for measuring Lipoprotein Lipase (LPL) activity.

How the assay works
Lipoprotein Lipase Assay Kit (Fluorometric) contains a quenched substrate that fluoresces upon hydrolysis by lipoprotein lipase (LPL). The fluorometric intensity is directly proportional to the amount of substrate hydrolyzed.

This lipase assay detects total lipase activity when no inhibitor is used. Comparing results in the presence or absence of an LPL inhibitor allows for quantification of LPL activity specifically.

Our results indicate that the majority (~90%) of lipase activity detected by this kit in post-heparin treated mouse plasma is from LPL. To determine the exact LPL specific activity in mouse plasma, measure activity in pre- and post-heparin treated plasma.

Lipoprotein Lipase assay summary:
- Add samples and standards to wells
- Add reaction mix
- Pre-incubate the plate at 37°C for 10 minutes
- Measure fluorescence (Ex/Em= 482/515 nm) in a kinetic mode
for 1 hour at 37°C

How other researchers are using ab204721
Lipoprotein Lipase Assay Kit (Fluorometric) has been used in a variety of sample type including:
- Human Caco-2 cells ¹
- Mouse Adipose tissue homogenate ²
- Mouse spleen samples ³
References: 1-Pang G al. 2022, 2-Gencer et al. 2021, 3-Ringel A et al. 2020

Other Notes
This product was previously called K721 Lipoprotein Lipase Activity Fluorometric Assay Kit. Biovision was acquired by Abcam in 2021.

Lipoprotein lipase (LPL) is a member of the lipase family that hydrolyzes triglycerides in chylomicrons and very low-density lipoprotein (VLDL). Digestion of triglycerides in VLDL by LPL leads to their conversion to intermediate-density lipoprotein (IDL) and then low-density lipoprotein (LDL). LPL is found attached to the luminal surface of endothelial cells in the heart, muscle, and adipose tissue. Mutations in lipoprotein lipase can lead to a variety of disorders such as lipoprotein metabolism, hypertriglyceridemia etc. Overexpression of LPL in mice has been shown to promote obesity and insulin resistance.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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规格

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab204721-100Test", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"LPL Positive Control", "number":"AB204721-CMP03", "productcode":"" }, { "size":"1 x 5 mL", "name":"LPL Assay Buffer", "number":"AB204721-CMP01", "productcode":"" }, { "size":"1 x 10 µL", "name":"LPL Substrate", "number":"AB204721-CMP04", "productcode":"" }, { "size":"1 x 20 µL", "name":"LPL Inhibitor", "number":"AB204721-CMP02", "productcode":"" } ] } } }

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性能和储存信息

运输条件

Blue Ice

储存信息

Please refer to protocols

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产品实验方案

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靶点信息

Key enzyme in triglyceride metabolism. Catalyzes the hydrolysis of triglycerides from circulating chylomicrons and very low density lipoproteins (VLDL), and thereby plays an important role in lipid clearance from the blood stream, lipid utilization and storage (PubMed : 11342582, PubMed : 27578112, PubMed : 8675619). Although it has both phospholipase and triglyceride lipase activities it is primarily a triglyceride lipase with low but detectable phospholipase activity (PubMed : 12032167, PubMed : 7592706). Mediates margination of triglyceride-rich lipoprotein particles in capillaries (PubMed : 24726386). Recruited to its site of action on the luminal surface of vascular endothelium by binding to GPIHBP1 and cell surface heparan sulfate proteoglycans (PubMed : 11342582, PubMed : 27811232).

See full target information LPL

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文献 (15)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:2430 PubMed40069146

2025

SR-A3 suppresses AKT activation to protect against MAFLD by inhibiting XIAP-mediated PTEN degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Pingping Lai,Guolin Miao,Yinqi Zhao,Yufei Han,Yanwei Li,Yiran Liu,Jiabao Guo,Wenxi Zhang,Xin Guo,Yitong Xu,Lianxin Zhang,Gonglie Chen,Zihao Zhou,Si Mei,Jingxuan Chen,Jinxuan Chen,Luzheng Xu,Chong Zhang,Yang Ding,Xiaoguang Dou,Shengmei Wen,Sin Man Lam,Guanghou Shui,Yuhui Wang,Wei Huang,Dongyu Zhao,Xunde Xian

International journal of molecular sciences 25: PubMed38612519

2024

Gene Silencing of Angiopoietin-like 3 (ANGPTL3) Induced De Novo Lipogenesis and Lipid Accumulation in Huh7 Cell Line.

Applications

Unspecified application

Species

Unspecified reactive species

Ilaria Rossi,Giorgia Marodin,Maria Giovanna Lupo,Maria Pia Adorni,Bianca Papotti,Stefano Dall'Acqua,Nicola Ferri

Theranostics 14:2036-2057 PubMed38505614

2024

Depletion of ApoA5 aggravates spontaneous and diet-induced nonalcoholic fatty liver disease by reducing hepatic NR1D1 in hamsters.

Applications

Unspecified application

Species

Unspecified reactive species

Jiabao Guo,Guolin Miao,Wenxi Zhang,Haozhe Shi,Pingping Lai,Yitong Xu,Lianxin Zhang,Gonglie Chen,Yufei Han,Ying Zhao,Geroge Liu,Ling Zhang,Yuhui Wang,Wei Huang,Xunde Xian

Nature communications 14:2656 PubMed37160898

2023

Resveratrol intervention attenuates chylomicron secretion via repressing intestinal FXR-induced expression of scavenger receptor SR-B1.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Pang,Fitore Raka,Alya Abbas Heirali,Weijuan Shao,Dinghui Liu,Jianqiu Gu,Jia Nuo Feng,Chieko Mineo,Philip W Shaul,Xiaoxian Qian,Bryan Coburn,Khosrow Adeli,Wenhua Ling,Tianru Jin

British journal of pharmacology 180:1803-1818 PubMed36735592

2023

The heparan sulfate mimetic Muparfostat aggravates steatohepatitis in obese mice due to its binding affinity to lipoprotein lipase.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Zhang,Kai Li,Hao-Ran Sun,Shao-Kun Sun,Ya-Ting Zhu,Yu-Ting Ge,Yu-Xuan Wu,Qin-Yao Zhou,Guan-Ting Li,Xiao-Ai Chang,Peng Sun,Ying Ding,Xiao Han

Frontiers in immunology 13:999470 PubMed36110841

2022

CRISPR/Cas9 based blockade of IL-10 signaling impairs lipid and tissue homeostasis to accelerate atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Haozhe Shi,Jiabao Guo,Qiongyang Yu,Xinlin Hou,Lili Liu,Mingming Gao,Lili Wei,Ling Zhang,Wei Huang,Yuhui Wang,George Liu,Peter Tontonoz,Xunde Xian

Biomedicines 9: PubMed33917642

2021

Adipocyte-Specific ACKR3 Regulates Lipid Levels in Adipose Tissue.

Applications

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Species

Unspecified reactive species

Selin Gencer,Yvonne Döring,Yvonne Jansen,Soyolmaa Bayasgalan,Olga Schengel,Madeleine Müller,Linsey J F Peters,Christian Weber,Emiel P C van der Vorst

Molecular metabolism 49:101197 PubMed33647469

2021

Adipocyte integrin-linked kinase plays a key role in the development of diet-induced adipose insulin resistance in male mice.

Applications

Unspecified application

Species

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Aimée R Bugler-Lamb,Annie Hasib,Xiong Weng,Chandani K Hennayake,Chenshi Lin,Rory J McCrimmon,Roland H Stimson,Michael L J Ashford,David H Wasserman,Li Kang

The Journal of pharmacology and experimental therapeutics 376:428-435 PubMed33443084

2021

RNA Interference Targeting Liver Angiopoietin-Like Protein 3 Protects from Nephrotic Syndrome in a Rat Model Via Amelioration of Pathologic Hypertriglyceridemia.

Applications

Unspecified application

Species

Unspecified reactive species

Yitong Zhao,Masaki Goto,Nosratola D Vaziri,Mahyar Khazaeli,Han Liu,Nazli Farahanchi,Elham Khanifar,Ted Farzaneh,Patrick A Haslett,Hamid Moradi,Mangala M Soundarapandian

Cell 183:1848-1866.e26 PubMed33301708

2020

Obesity Shapes Metabolism in the Tumor Microenvironment to Suppress Anti-Tumor Immunity.

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Unspecified application

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Alison E Ringel,Jefte M Drijvers,Gregory J Baker,Alessia Catozzi,Juan C García-Cañaveras,Brandon M Gassaway,Brian C Miller,Vikram R Juneja,Thao H Nguyen,Shakchhi Joshi,Cong-Hui Yao,Haejin Yoon,Peter T Sage,Martin W LaFleur,Justin D Trombley,Connor A Jacobson,Zoltan Maliga,Steven P Gygi,Peter K Sorger,Joshua D Rabinowitz,Arlene H Sharpe,Marcia C Haigis

View all publications

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Lipoprotein Lipase Assay试剂盒(Fluorometric)

Lipoprotein Lipase Assay Kit (Fluorometric)

Functional Studies - Lipoprotein Lipase Assay Kit (Fluorometric) (AB204721)

Functional Studies - Lipoprotein Lipase Assay Kit (Fluorometric) (AB204721)

Functional Studies - Lipoprotein Lipase Assay Kit (Fluorometric) (AB204721)

Functional Studies - Lipoprotein Lipase Assay Kit (Fluorometric) (AB204721)

Schematic Diagram - Lipoprotein Lipase Assay Kit (Fluorometric) (AB204721)