Lipid Peroxidation (MDA) Assay试剂盒(Colorimetric/Fluorometric) (ab118970)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 1 hr 20 min
- Sample type: Cell culture extracts, Plasma, Tissue Extracts, Urine (UTI)
- Sensitivity: 0.1 nmol/well
概述
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产品名称
Lipid Peroxidation (MDA) Assay试剂盒(Colorimetric/Fluorometric)
参阅全部 Lipid Peroxidation 试剂盒 -
检测方法
Colorimetric/Fluorometric -
样品类型
Plasma, Cell culture extracts, Tissue Extracts, Urine (UTI) -
检测类型
Quantitative -
灵敏度
> 0.1 nmol/well -
检测时间
1h 20m -
产品概述
Lipid Peroxidation (MDA) Assay Kit (Colorimetric/Fluorometric) (ab118970) provides a convenient tool for sensitive detection of malondialdehyde (MDA).
How the assay works
In the lipid peroxidation assay protocol, the MDA in the sample reacts with thiobarbituric acid (TBA) to generate a MDA-TBA adduct. The MDA-TBA adduct can be easily quantified colorimetrically (OD = 532 nm) or fluorometrically (Ex/Em = 532/553 nm). This assay detects MDA levels as low as 1 nmol/well colorimetrically and 0.1 nmol/well fluorometrically.
Lipid peroxidation assay protocol summary
- Add TBA solution to samples and standards, incubate at 95°C for 60 min, cool in ice bath for 10 min
- Transfer to wells of microplate
- Analyze with microplate reader
For higher sensitivity, precipitate with n-butanol, centrifuge, dry and resuspend pellet before analysis.
Related MDA assay products
For an alternative MDA assay, without the heating steps required in the TBARS assay, try MDA assay ab233471.
Getting the best performance from ab118970
Technical Note: MDA is unlikely to be detectable in healthy urine but may be elevated in the presence of infection or disease of the urinary tract.
How other researchers are using Lipid Peroxidation Assay Kit ab118970
The MDA/TBARs assay kit has been used in publications in a variety of sample types, including:
- Human: serum1, hippocampal primary cell extracts2, A375 cultured cell lysates3, plasma and platelet samples4
- Mouse: neuronal cell lysates5, heart tissue extract6, plasma7, cell extracts8
- Rat: hippocampal tissue extracts9, cardiomyocyte extracts of cultured cells10, lung lysates11
- Pig: serum12
References: 1 - Shen J et al. 2018, 2 - Wang Q et al. 2019, 3 - Luo M et al. 2018, 4 - Mustafa AG et al. 2018, 5 - Murphy K et al. 2018, 6 - Guan F et al. 2019, 7 - Costa CRC et al. 2018, 8 - Eleftheriadis T et al. 2019, 9 - Malekiyan et al. 2019, 10 - Zhou Z et al. 2018, 11 - Li L et al. 2018, 12 - Lee SE and Kang KS 2019
Related and recommended products
Also see the popular 4-HNE Assay Kit ab238538 as an alternative marker of lipid peroxidation and oxidative stress.
Iron Assay Kit ab83366 is often used in combination with Lipid Peroxidation (MDA) Assay Kit ab118970 and GSH/ GSSG Assay Kit ab239709 in the study of ferroptosis.
Background information
Lipid peroxidation refers to the oxidative degradation of lipids. In this process free radicals take electrons from the lipids (generally in cell membranes), resulting in cell damage. Quantification of lipid peroxidation is essential to assess oxidative stress. Lipid peroxidation forms reactive aldehydes such as malondialdehyde (MDA) and 4-hydroxynonenal (4- HNE) as natural bi-products. Malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE) are often used as markers of lipid peroxidation, and to assay for oxidative damage / oxidative stress.
The MDA assay is also refered to as a TBARS assay. The TBARS assay (thiobarbituric acid reactive substance assay) is used to measure lipid peroxidation in cell and tissue extracts, and biological fluids. The TBARS assay detects the level of MDA (malondialdehyde), the major lipid oxidation product, and also some minor related compounds. It is often considered a good index of the level of oxidative stress in a biological sample.
Other notes
This product is manufactured by BioVision, an Abcam company and was previously called K739 Lipid Peroxidation (MDA) Colorimetric/Fluorometric Assay Kit. K739-100 is the same size as the 100 test size of ab118970. -
说明
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the SDS download section.
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平台
Microplate reader
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 100 tests 2000 tests BHT Stock 1 x 1ml 20 x 1ml MDA Lysis Buffer 1 x 25ml 20 x 25ml MDA Standard 1 x 100µl 20 x 100µl Phosphotungstic Acid Solution 1 x 12.5ml 20 x 12.5ml Developer VII 4 vials 80 vials -
研究领域
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相关性
Lipid peroxidation refers to the oxidative degradation of lipids and is a well-defined mechanism of cellular damage. The formation of lipid peroxidation products leads to spread of free radical reactions leading to cell damage. -
别名
- TBAR
- TBARS
相关产品
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Related Products
图片
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MDA assay standard curve
Typical MDA standard calibration curve using fluorometric reading.
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MDA assay standard curveTypical MDA standard calibration curve using colorimetric reading.
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Lipid Peroxidation Assay performed on mouse sciatic nerve samplesImage courtesy of Hichor et al. Sci Rep. 2018; 8: 2524. doi: 10.1038/s41598-018-20980-3. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/.Hichor M et al. used the TBARS assay / MDA assay ab118970 to study the role of LXRs in the regulation of oxidative stress in peripheral nerves.They identified that in sciatic nerves in LXR knockout mice (LXRdKO), the MDA concentration was significantly increased, and that this was corrected by the treatment of mice with the anti-oxidant ROS scavenger N-acetylcysteine (NAC). -
Lipid Peroxidation measured with MDA assay in Fabry patients and healthy controlsImage courtesy of Ravarotto V et al. PLoS One. 2018; 13(9): e0204618. doi: 10.1371/journal.pone.0204618. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/.Ravarotto V et al. used Lipid Peroxidation Assay Kit ab118970 to assess oxidative stress in Fabry disease. They identified that MDA levels are higher in Fabry patients, indicating higher levels of oxidative stress.
The MDA concentration was measured in plasma from Fabry patients compared to healthy control patients. Data are shown ±SEM. *: p = 0.01.
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Functional Studies - Lipid Peroxidation (MDA) Assay Kit (ab118970)Measurement of MDA in human plasma (20 µl) and rat liver lysate (10 mg).
数据表及文件
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SDS download
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Datasheet download
文献 (545)
ab118970 被引用在 545 文献中.
- Fouad H et al. Use of Mesenchymal Stem Cells in Experimental Ovarian Damage. Curr Stem Cell Res Ther 19:725-734 (2024). PubMed: 37448361
- Bi R et al. Butyrate enhances erastin-induced ferroptosis of lung cancer cells via modulating the ATF3/SLC7A11 pathway. Environ Toxicol 39:529-538 (2024). PubMed: 37341073
- Ji M et al. Vinpocetine improves dyskinesia in Parkinson's disease rats by reducing oxidative stress and activating the Wnt/β-catenin signaling pathway. Chem Biol Drug Des 103:e14358 (2024). PubMed: 37749299
- Qin H et al. Tanshinone IIA ameliorates cisplatin-induced toxicology and cisplatin resistance via regulating SLC7A11 expression. Environ Toxicol 39:1429-1441 (2024). PubMed: 37987512
- Niu T et al. Porous Se@SiO2 nanospheres alleviate diabetic retinopathy by inhibiting excess lipid peroxidation and inflammation. Mol Med 30:24 (2024). PubMed: 38321393