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AB169557

L-Lactate Assay试剂盒(Fluorometric，High Sensitivity)

L-Lactate Assay Kit (Fluorometric, High Sensitivity)

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(1 Review)

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(11 Publications)

L-Lactate Assay Kit ab169557 is suitable for measuring very low levels of L(+)-lactate in a variety of samples.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
4 Images
Functional Studies - L-Lactate Assay Kit (Fluorometric, High Sensitivity) (AB169557)
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Supplier Data

Functional Studies - L-Lactate Assay Kit (Fluorometric, High Sensitivity) (AB169557)

Lactate Levels in Various Samples. Measurement of Lactate levels in rat liver (1.2 µg), kidney (0.7 µg), muscle (0.45 µg) and in human serum (0.5 µL from 1 : 10 diluted serum). Assays were performed following kit protocol.

Functional Studies - L-Lactate Assay Kit (Fluorometric, High Sensitivity) (AB169557)
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Lab

Functional Studies - L-Lactate Assay Kit (Fluorometric, High Sensitivity) (AB169557)

Relative signal (RFU) in filtered mouse heart lysate (3.7 mg protein/mL prior to filtration) in different volumes, comparing L-Lactate signals (black) with background reading (white, no enzyme).

Functional Studies - L-Lactate Assay Kit (Fluorometric, High Sensitivity) (AB169557)
  • FuncS

Lab

Functional Studies - L-Lactate Assay Kit (Fluorometric, High Sensitivity) (AB169557)

Standard curve with background signal subtracted (duplicates; +/- SD).

Functional Studies - L-Lactate Assay Kit (Fluorometric, High Sensitivity) (AB169557)
  • FuncS

Lab

Functional Studies - L-Lactate Assay Kit (Fluorometric, High Sensitivity) (AB169557)

L-Lactate measured in unfiltered human plasma (dilution range 1 : 30-1 : 90) and mouse serum (dilution range 1 : 2500-1 : 7500); duplicates +/- SD. The background control levels (no enzyme) were below level of detection.

关键信息

检测方法

Fluorescent

样品类型

Plasma, Tissue, Suspension cells, Serum, Other biological fluids, Adherent cells

检测类型

Quantitative

灵敏度

< 0.2 µM

范围

0.05 - 0.25 nmol/well

检测平台

Microplate reader

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产品详情

L-Lactate Assay Kit (ab169557) is suitable for measuring very low levels of L(+)-lactate in a variety of samples.

In this assay, L(+)-lactate is specifically oxidized to form an intermediate that reacts with a colorless probe to generate fluorescence (Ex/Em = 535/587 nm), which is directly proportional to the amount of lactate.

This simple, rapid and high-throughput suitable assay kit is the most sensitive lactate assay kit on the market. It can detect L(+)-lactate less than 0.2 uM in a variety of biological samples.

Other Notes
L(+)-Lactate is the major stereo-isomer of lactate formed in human intermediary metabolism and is present in blood. D(-)-Lactate is also present (see ) but only at about 1-5% of the concentration of L(+)-Lactate.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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规格

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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
-20°C
储存信息
-20°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

L-Lactate also known as lactate is a byproduct of anaerobic glycolysis where it plays an important role in energy metabolism. L-Lactate is a small molecule with a molecular mass of approximately 90.08 g/mol. It forms in various tissues like muscle cells during intense exercise when oxygen levels are low. This process leads to a conversion of pyruvate to lactate by the action of the enzyme lactate dehydrogenase (LDH) which is present in many tissues with higher expressions in muscles and heart.
Biological function summary

L-Lactate acts as a signaling molecule which affects cellular functions and contributes to metabolic regulation. It is not part of a complex but serves as an important intermediate in metabolic pathways. L-Lactate provides energy to cells by converting back to pyruvate in the presence of oxygen which then enters the citric acid cycle. This conversion and its utilization as energy play important roles in balancing cellular energy demands especially under hypoxic conditions.

Pathways

L-Lactate links to critical processes like glycolysis and the Cori cycle. During glycolysis pyruvate may convert to L-Lactate under anaerobic conditions to regenerate NAD+ necessary for glycolysis to continue. In the Cori cycle lactate produced by anaerobic glycolysis in muscles is released into the bloodstream and transported to the liver. There it converts back to glucose supporting gluconeogenesis. These processes highlight the close involvement of L-Lactate with proteins such as lactate dehydrogenase and pyruvate kinase.

L-Lactate is associated with conditions like lactic acidosis and cancer. Lactic acidosis characterized by high lactate levels can occur due to oxygen deprivation or mitochondrial dysfunction. Meanwhile cancer cells often show enhanced glycolysis and lactate production known as the Warburg effect facilitating their growth. Proteins like hypoxia-inducible factor 1-alpha (HIF-1α) and lactate dehydrogenase (LDH) are key players in these conditions influencing lactate metabolism and potentially serving as therapeutic targets.
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产品实验方案

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靶点信息

See full target information L-lactate
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文献 (11)

Recent publications for all applications. Explore the full list and refine your search

Cell research 35:97-116 PubMed39757301

2025

Nonenzymatic lysine D-lactylation induced by glyoxalase II substrate SLG dampens inflammatory immune responses.

Applications

Unspecified application

Species

Unspecified reactive species

Qihang Zhao,Qiang Wang,Qinghua Yao,Zhengdong Yang,Wenfang Li,Xiaojie Cheng,Yingling Wen,Rong Chen,Junfang Xu,Xuanying Wang,Dexiang Qin,Shuyang Zhu,Liujie He,Nan Li,Yanfeng Wu,Yizhi Yu,Xuetao Cao,Pin Wang

BMC cancer 24:979 PubMed39118022

2024

Glycolysis-related genes predict prognosis and indicate immune microenvironment features in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Xu,Jin Liu,Yuanqing An,Lei Zhou,Hui Sun,Zhen Xu,Deqiang Wang,Zhanwen Liang,Caihua Xu,Bingyi Wang,Wei Li

Cancer medicine 13:e70031 PubMed39114948

2024

Mechanism and rational combinations with GP-2250, a novel oxathiazine derivative, in ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Mark S Kim,Deanna Glassman,Katelyn F Handley,Adrian Lankenau Ahumada,Nicholas B Jennings,Emine Bayraktar,Katherine Foster,Robiya Joseph,Sanghoon Lee,Robert L Coleman,Anil K Sood

Bioengineered 13:2554-2567 PubMed35156520

2022

LncRNA LINC00525 activates HIF-1α through miR-338-3p / UBE2Q1 / β-catenin axis to regulate the Warburg effect in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Fanqi Meng,Xiaofan Luo,Chenyao Li,Guangyi Wang

International journal of molecular sciences 22: PubMed34299176

2021

Increased Beta-Hydroxybutyrate Level Is Not Sufficient for the Neuroprotective Effect of Long-Term Ketogenic Diet in an Animal Model of Early Parkinson's Disease. Exploration of Brain and Liver Energy Metabolism Markers.

Applications

Unspecified application

Species

Unspecified reactive species

Katarzyna Z Kuter,Łukasz Olech,Urszula Głowacka,Martyna Paleczna

Cell reports 34:108678 PubMed33503424

2021

Metabolic compensation activates pro-survival mTORC1 signaling upon 3-phosphoglycerate dehydrogenase inhibition in osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Richa Rathore,Katharine E Caldwell,Charles Schutt,Caitlyn B Brashears,Bethany C Prudner,William R Ehrhardt,Cheuk Hong Leung,Heather Lin,Najat C Daw,Hannah C Beird,Abigail Giles,Wei-Lien Wang,Alexander J Lazar,John S A Chrisinger,J Andrew Livingston,Brian A Van Tine

EMBO molecular medicine 12:e12423 PubMed32875720

2020

Dietary magnesium supplementation improves lifespan in a mouse model of progeria.

Applications

Unspecified application

Species

Unspecified reactive species

Ricardo Villa-Bellosta

Cancer cell international 19:252 PubMed31582908

2019

Circular RNA CDR1as sponges miR-7-5p to enhance E2F3 stability and promote the growth of nasopharyngeal carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Qiong Zhong,Juncong Huang,Jiawang Wei,Renrui Wu

Journal of neurochemistry 148:63-79 PubMed30295916

2018

Astrocyte support is important for the compensatory potential of the nigrostriatal system neurons during early neurodegeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Katarzyna Kuter,Łukasz Olech,Urszula Głowacka,Martyna Paleczna

FEBS letters 590:2076-85 PubMed27264935

2016

The tumor suppressor gene lkb1 is essential for glucose homeostasis during zebrafish early development.

Applications

Unspecified application

Species

Unspecified reactive species

Xia Kuang,Chao Liu,Junshun Fang,Weirui Ma,Jian Zhang,Sheng Cui
View all publications
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