KDM4/JMJD2 Activity Quantification Assay试剂盒(Colorimetric) (ab113461)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 3 hr
概述
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产品名称
KDM4/JMJD2 Activity Quantification Assay试剂盒(Colorimetric)
参阅全部 KDM4/JMJD2 试剂盒 -
检测方法
Colorimetric -
检测类型
Enzyme activity -
检测时间
3h 00m -
种属反应性
与反应: Mouse, Human
预测可用于: Mammals
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产品概述
KDM4/JMJD2 (Lysine-specific demethylase 4) is a family of histone demethyltransferases involved in H3(meK9) specific demethylation. The KDM4/JMJD2 family are JmjC proteins that specifically removes tri-methylation from H3(meK9) and H3(meK36) but has no activity on mono- or di-methylated residues.
KDM4/JMJD2 Activity Quantification Assay Kit (Colorimetric) (ab113461) is designed for the easy and fast colorimetric measurement of KDM4/JMJD2 activity or inhibition. The antibody-based, immunospecific method directly detects KDM4/JMJD2-converted demethylated products, rather than by-products, in a 96 stripwell microplate format.
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平台
Microplate reader
性能
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存放说明
Please refer to protocols. -
组件 48 tests 96 tests 10X Wash Buffer 1 x 14ml 1 x 28ml 8-Well Assay Strips (with Frame) 6 units 12 units Adhesive Covering Film 1 unit 1 unit Capture Antibody, 1000 µg/mL 1 x 5µl 1 x 10µl Co-factor 1 1 x 30µl 1 x 60µl Co-factor 2 1 x 30µl 1 x 60µl Co-factor 3 1 x 30µl 1 x 60µl Detection Antibody, 400 µg/mL 1 x 6µl 1 x 12µl Developer Solution 1 x 5ml 1 x 10ml JMJD2 Assay Buffer 1 x 4ml 1 x 8ml JMJD2 Assay Standard, 50 µg/mL 1 x 10µl 1 x 20µl JMJD2 Substrate, 50 µg/mL 1 x 60µl 1 x 120µl Stop Solution 1 x 5ml 1 x 10ml -
研究领域
相关产品
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Related Products
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- KDM5/JARID Activity Quantification Assay Kit (Colorimetric) (ab113463)
- KDM5/JARID Activity Quantification Assay Kit (Fluorometric) (ab113464)
- Nuclear Extraction Kit (ab113474)
图片
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Functional Studies - KDM4/JMJD2 Activity Quantification Assay Kit (Colorimetric) (ab113461)Demonstration of high sensitivity of the KDM4/JMJD2 activity assay achieved by using recombinant KDM4/JMJD2 with ab113461.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab113461 被引用在 1 文献中.
- Martinez CA et al. Intermittent hypoxia enhances the expression of hypoxia inducible factor HIF1A through histone demethylation. J Biol Chem 298:102536 (2022). PubMed: 36174675