KDM1/LSD1 Activity Quantification Assay试剂盒(Fluorometric) (ab113460)

Thank you for contacting us.
The kit ab113472 is designed to detect DNA demethylase activity and will detect the this activity using a unique methylated DNA substrate. The detection is then colorimetric and can be measured by optical density measure at 450nm. I do not think that this is adapted for measuring LSD1 activity.
We do however have different other kits, which might correspond more to what you are looking for:
We do have the EpiSeeker KDM1/LSD1 activity quantification kit which comes either colorimetric (ab113459) or fluorometric (ab113460).  This kit does measure the activity of the LSD1 by using the di-methylated histone H3-K4 as a substrate for LSD1. The fluorimetric assay is read at Ex/Em = 530/590 nm.
https://www.abcam.com/KDM1LSD1-Activity-Quantification-Assay-Kit-Colorimetric-ab113459.html
https://www.abcam.com/KDM1LSD1-Activity-Quantification-Assay-Kit-Fluorometric-ab113460.html
Alternatively we do also have the EpiSeeker Histone Demethylase KDM1/LSD1 Activity Quantification Assay Kit ab113457. This kit does detect the histone demethylase by indirect detection. https://www.abcam.com/Histone-Demethylase-KDM1LSD1-Activity-Quantification-Assay-Kit-ab113457.html
It might be interesting to note that we do have a website dedicated to the EpiSeeker products. Please feel free to browse this site as well. www.abcam.com/episeeker
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for contacting Abcam. For ab113459 and ab113460, both kits directly detects KDM1/LSD1-converted demethylated products, rather than by-products, in a 96 stripwell microplate format. The main difference between the 2 are that ab113459, has a colorimetric detection systems, whereas, ab113460 has a fluorescent detections method. Depending on the equipment available in your lab, would determine which one of these you would choose. The ab113457 measures histone demethylase KDM1/LSD1 activity/inhibition from a broad range of species including mammalian cells/tissues, plants, and bacteria and this is an indirect detection method, compared to the above kits. For ab113456, this kit is ready-to-use and provides all the essential components needed for screening KDM1/LSD1 inhibitors, which are directly interacting with the enzyme or blocking the binding to its substrate. You can find more information about these products and how to choose the correct kit for your needs, by clicking on the following link: https://www.abcam.com/index.htmlpageconfig=resource&rid=14469&source=pagetrap&viapagetrap=episeeker#3 If there is anything else I can help you with, please let me know.

Thank you for contacting Abcam. I have heard back from the lab about your inquiry as to whether you can use the products of your IP reaction with the kit ab113460. They have informed me the kit ab113460 can be used with IP product containing LSD1/antibody/beads complex. The assay can be performed according to the protocol of the kit with increased wash time ( additional 2-3 wash time) after incubation of the complex and substrate (Step 2f of the protocol), as the beads ( especially magnetic beads) could non-specifically bind to the wells that causes the antibody in the complex to bind to the detection antibody and generates a signal at the late steps. If there is anything else I can help you with, please do not hesitate to contact me.  

Thank you for contacting us. In answer to your question, the EpiSeeker KDM1/LSD1 Activity Quantification Assay Kit (ab113460) can be used to test LSD1 inhibitors both in vivo and in vitro. As you suggested, for in vivo testing, the cells can be incubated with the inhibitors (inhibitor compounds need to be capable of entering into the cells) for a defined time period. Nuclear extracts of these cells can then be used for the measurement of LSD1 activity with an untreated cell sample tested in parallel as a standard. The inhibitory effect can then be calculated by comparing the activity of the treated samples to that of untreated control. For in vitro testing, inhibitors can be directly incubated with the nuclear extract or purified LSD1 enzyme and then the level of inhibition calculated by comparing with untreated samples. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.