Inorganic Polyphosphate Assay试剂盒(Fluorometric) (ab284528)
Key features and details
- Assay type: Cell-based (quantitative)
- Detection method: Fluorescent
- Platform: Microplate (12 x 8 well strips)
- Sample type: Cell Lysate, Tissue Lysate
概述
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产品名称
Inorganic Polyphosphate Assay试剂盒(Fluorometric) -
检测方法
Fluorescent -
样品类型
Cell Lysate, Tissue Lysate -
检测类型
Cell-based (quantitative) -
实验步骤
Multiple steps standard assay -
产品概述
Inorganic Polyphosphate Assay Kit (Fluorometric) (ab284528) (K2025) is a simple assay to quantify the amount of Poly P in various biological samples. The assay uses a fluorescent dye that forms a complex with Poly P present in the samples and the fluorsecent complex is measured at Ex/Em = 415/550 nm.
The fluorescent signal is proportional to the Poly P concentration in the samples.
This kit provides a rapid, sensitive and a convenient tool for detecting Poly P. It can detect as low as 50 pmole under the assay conditions.
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说明
This product is manufactured by BioVision, an Abcam company and was previously called K2025 Inorganic Polyphosphate Assay Kit (Fluorometric). K2025-100 is the same size as the 100 test size of ab284528.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the SDS download section.
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经测试应用
适用于: Functional Studiesmore details -
平台
Microplate (12 x 8 well strips)
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 100 tests Buffer III 1 x 30ml DNase 1 x 400µl Polyphosphate Assay Buffer 1 x 100ml Polyphosphate Dye 1 vial Polyphosphate Standard 1 vial Proteinase K 1 x 200µl RNAse Positive Control 1 x 400µl
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab284528于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
Functional Studies
Use at an assay dependent concentration. |
图片
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Inorganic Polyphosphate Assay
Poly P Standard Curve.
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Inorganic Polyphosphate AssayPoly P concentration in rat brain tissue and E. coli cells after osmotic stress assayed according to the kit protocol.
数据表及文件
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SDS download
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Datasheet download
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