In situ BrdU DNA Fragmentation Assay试剂盒(ab66098)
Key features and details
- Detection method: Flow cytometry-fluorescent
- Platform: Flow cytometer, Fluorescence microscope
- Assay time: 3 hr
- Sample type: Cell culture extracts, Tissue sections
概述
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产品名称
In situ BrdU DNA Fragmentation Assay试剂盒
参阅全部 DNA fragmentation 试剂盒 -
检测方法
Flow cytometry-fluorescent -
样品类型
Cell culture extracts, Tissue sections -
检测时间
3h 00m -
种属反应性
与反应: Mammals -
产品概述
InSitu BrdU DNA Fragmentation Assay Kit provides complete components including positive and negative control cells for conveniently detecting DNA fragmentation by fluorescence microscopy or flow cytometry. The kit utilizes Br-dUTP (bromolated deoxyuridine triphosphate nucleotides) which is more readily incorporated into DNA strand breaks than other larger ligands (e.g., fluorescein, biotin or digoxigenin). The greater incorporation gives rise to brighter signal when the Br-dUTP sites are identified by a fluorescein labeled anti-BrdU monoclonal antibody.
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平台
Flow cytometer, Fluorescence microscope
性能
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存放说明
Please refer to protocols. -
组件 60 tests Anti-BdU-FITC Antibody 1 x 0.3ml Br dUTP 1 x 0.48ml Negative Control Cells 1 x 5ml PI/Nase Staining buffer 1 x 30ml Positive Control Cells 1 x 5ml Reaction buffer 1 x 0.6ml Rinse buffer 1 x 120ml TdT Enzymes 1 x 45µl Wash buffer 1 x 120ml -
研究领域
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相关性
Internucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells.
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab66098 被引用在 7 文献中.
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- Sun X et al. Neutralization of Oxidized Phospholipids Ameliorates Non-alcoholic Steatohepatitis. Cell Metab 31:189-206.e8 (2020). PubMed: 31761566
- Guo L et al. Hepatic neuregulin 4 signaling defines an endocrine checkpoint for steatosis-to-NASH progression. J Clin Invest 127:4449-4461 (2017). PubMed: 29106384
- Waldschmitt N et al. C/EBP homologous protein inhibits tissue repair in response to gut injury and is inversely regulated with chronic inflammation. Mucosal Immunol 7:1452-66 (2014). PubMed: 24850428
- Mahajan K et al. Ack1-mediated androgen receptor phosphorylation modulates radiation resistance in castration-resistant prostate cancer. J Biol Chem 287:22112-22 (2012). PubMed: 22566699
- Van de Velde S et al. mTOR links incretin signaling to HIF induction in pancreatic beta cells. Proc Natl Acad Sci U S A 108:16876-82 (2011). PubMed: 21949366