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Histone H3 Total Quantification试剂盒(Colorimetric) (ab115091)

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Functional Studies - Histone H3 Quantification Assay Kit (Colorimetric) (ab115091)

    Key features and details

    • Detection method: Colorimetric
    • Platform: Microplate reader
    • Assay time: 2 hr 30 min
    • Sample type: Adherent cells, Suspension cells, Tissue
    • Sensitivity: 10 ng/well

    概述

    • 产品名称

      Histone H3 Total Quantification试剂盒(Colorimetric)
      参阅全部 Histone H3 试剂盒

    • 检测方法

      Colorimetric

    • 样品类型

      Tissue, Adherent cells, Suspension cells

    • 灵敏度

      10 ng/well

    • 范围

      20 ng/well - 1000 ng/well

    • 检测时间

      2h 30m

    • 种属反应性

      与反应: Mouse, Rat, Human
      预测可用于: Mammals

    • 产品概述

      Histone H3 Total Quantification Kit (Colorimetric) (ab115091) enables the user to quantify levels of histone H3 proteins independent of their modified states. It can also be used for normalizing the modified histone H3 content of samples when run in parallel with Abcam's histone modification quantification kit series. ab115091 is suitable for specifically measuring total histone H3 on mammalian samples such as human, mouse, and rat, including fresh and frozen tissues and cultured adherent and suspension cells.


      Histone H3 can undergo several epigenetic modifications that influence cellular processes such as transcription activation/inactivation, chromosome packaging, and DNA damage/repair. These modifications including acetylation, phosphorylation or methylation occur on the N-terminal tail domains of histone H3 through a variety of histone modifying enzymes. In most species, histone H3 is primarily acetylated at K9, K14, K18, K23 and K56, methylated at K4, K9, K27, K36 and K79, and phosphorylated at S10, S28, T3 and T11, respectively.

    • 平台

      Microplate reader

    性能

    图片

    • Functional Studies - Histone H3 Quantification Assay Kit (Colorimetric) (ab115091)
      Functional Studies - Histone H3 Quantification Assay Kit (Colorimetric) (ab115091)

      Measurement of histone H3 with different concentrations of the included H3 standard in theHistone H3 Total Quantification Kit (Colorimetric) (ab115091).

    文献 (1)

    发表研究结果有使用 ab115091？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab115091 被引用在 1 文献中.

    • Chen Y  et al. Remodeling of the H3 nucleosomal landscape during mouse aging. Transl Med Aging 4:22-31 (2020). PubMed: 32462102

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