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Histone Demethylase (H3K4) Activity Quantification Assay试剂盒(ab113455)

Submit a review Q&A (1)References (2)
Functional Studies - Histone Demethylase (H3K4) Activity Quantification Assay Kit (ab113455)

    Key features and details

    • Assay type: Enzyme activity
    • Platform: Microplate reader
    • Assay time: 3 hr
    • Sample type: Cell Lysate, Tissue Extracts

    概述

    • 产品名称

      Histone Demethylase (H3K4) Activity Quantification Assay试剂盒

    • 样品类型

      Tissue Extracts, Cell Lysate

    • 检测类型

      Enzyme activity

    • 检测时间

      3h 00m

    • 种属反应性

      与反应: Mouse, Human, Plants
      预测可用于: Mammals, Other species

    • 产品概述

      Histone Demethylase (H3K4) Activity Quantification Assay Kit (ab113455) allows the user to measure activity/inhibition of H3K4 specific histone demethylases using cell/ tissue extracts. The kit is ready-to-use and provides all the essential components needed for measuring histone demethylase H3K4 specific activity/inhibition from a broad range of species including mammalian cells/tissues, plants, and bacteria.


      Methylation of histone H3 at lysine 4 (H3K4) is thought to be a global epigenetic mark in euchromatin and a mediator of activated transcription. H3K4 demethylation is regulated by KDM1/LSD1, which can remove di- and mono-methylation from H3K4, and the JARID proteins such as RBP2 which catalyzes the removal of methylation through a hydroxylation reaction.


       

    • 平台

      Microplate reader

    性能

    图片

    • Functional Studies - Histone Demethylase (H3K4) Activity Quantification Assay Kit (ab113455)
      Functional Studies - Histone Demethylase (H3K4) Activity Quantification Assay Kit (ab113455)

      Nuclear extracts were prepared from Hela cells using the ab113475 and H3K4 specific histone demethyase activity was measured.

    文献 (2)

    发表研究结果有使用 ab113455？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab113455 被引用在 2 文献中.

    • Mi Y  et al. Lysine demethylase 5A promotes prostate adenocarcinoma progression by suppressing microRNA-330-3p expression and activating the COPB2/PI3K/AKT axis in an ETS1-dependent manner. J Cell Commun Signal 16:579-599 (2022). PubMed: 35581421
    • Jamshidi S  et al. KDM5B protein expressed in viable and fertile ?ARID mice exhibit no demethylase activity. Int J Oncol 59:N/A (2021). PubMed: 34713299

    客户评价及客户问答

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    Question

    We would like to quantify H3K4me3 demethylation activity in mouse embryonic stem cell extracts. You have two kits listed that seem to both serve that purpose: 113464 and 113455. - What is the difference between these two? - Do you recommend one over the other for K4me3 demethylation? - Has any of them been used in any publications?

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    Answer

    Thank you for your interest in ab113455 & ab113464.

    You are correct that both these kits measure demethylase activity. The major difference between these 2 kits is the substrate captured on the well. For ab113455, the substrate is a dimethylated histone H3-K4 substrate while the substrate used in ab113464 is a trimethylated histone H3-K4 substrate.

    Due to these different substrates, ab113455 will detect LSD1 type demethylase activity which can remove mono and dimethyl groups while ab113464 will detect JARID type demethylase activity which removes trimethyl groups from H3 K4.

    So it seems ab113464 would be the proper choice for your interest in studying H3K4me3 demethylation activity.

    As far as we know neither of these kits have been used in published works.

    I hope this is helpful. Please contact us again if you have any further questions.

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